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Registros recuperados : 20 | |
3. | | CARVALHO, A. G. de; WENDT, J. G. N.; LIMA, W. G.; BRASIL, F. da C. Parametros biologicos e consumo de area foliar de Urbanus acawoios (Willians, 1926) (Lepidoptera: Hespernidae) em Galactia striata (Jacq.) Ub (Leguminosae: Faboideae). Floresta e Ambiente, Rio de Janeiro, v.6, n.1, p.88-94, 1999. Biblioteca(s): Embrapa Florestas. |
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5. | | LIMA, W. G.; TECCHIO, M. A; CÓSER, G.; HAWERROTH, F. J.; ALBOLEA, M. N.; SANCHEZ, C. A. P. C. Superação da dormência com o uso do ERGER® associado a nitrato de cálcio em videiras da cultivar niagara rosada em São Manuel, SP. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UNESP, 29., 2017, Botucatu, SP. Anais... Botucatu, SP: UNESP, 2017. Biblioteca(s): Embrapa Uva e Vinho. |
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6. | | CARVALHO, A. G. de; SILVA, M. R. da; LIMA, W. G.; WENDT, J. G. N.; SILVA, L. K. F. da. Arborização com sombreiro, Clitoria fairchildiana: lepidópteros da família Hesperiidae associados. In: INTERNATIONAL CONGRESS AND EXHIBITION ON FOREST, 5., 1999, Curitiba. Forest 99: [resumos]. Rio de Janeiro: BIOSFERA, 1999. 1 CD-ROM. Autoria bilíngue: CONGRESSO E EXPOSICAO INTERNACIONAL SOBRE FLORESTAS, 5., 1999, Curitiba. Biblioteca(s): Embrapa Florestas. |
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11. | | VELOSO, J. S.; LIMA, W. G.; REIS, A.; DOYLE, V. P.; MICHEREFF, S. J.; CÂMARA, M. P. S. Factors influencing biological traits and aggressiveness of Colletotrichum species associated with cashew anthracnose in Brazil. Plant Pathology, v. 70, n. 1, p. 167-180, Jan. 2021. Biblioteca(s): Embrapa Hortaliças. |
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12. | | MENEZES, A. J. E. A. de; TRINDADE, D. R.; POLTRONIERI, L. S.; HOMMA, A. K. O.; LIMA, W. G. Ocorrência da fusariose na variedade de bananeira 'prata zulu' no sudeste paraense, município de Eldorado dos Carajás, Pará. In: SIMPÓSIO BRASILEIRO SOBRE BANANICULTURA, 5.; WORKSHOP DO GENOMA MUSA, 1., 2003, Paracatu, MG. Anais. Cruz das Almas: Nova Civilização, 2003. p. 167-169. Editado por Aristóteles Pires de Matos e Paulo Ernesto Meissner Filho. Biblioteca(s): Embrapa Amazônia Oriental. |
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14. | | VIEIRA, W. A. dos S.; LIMA, W. G.; NASCIMENTO, E. de S.; MICHEREFF, S. J.; REIS, A.; CAMARA, M. P. S.; DOYLE, V. Colletotrichum species associated with banana anthracnose in Brazil. In: BOTANY 2015 SCIENCE AND PLANTS FOR PEOPLE, 2015, Edmonton. Electronic Abstract Site Overview. St. Louis: Botanical Society of America, 2015. On line. Biblioteca(s): Embrapa Hortaliças. |
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15. | | COSTA, C. A. da; LIMA, W. G.; VELOSO, J. S.; JAIMES, F. M.; SOUZA, E. N.; MICHEREFF, S. J.; CÂMARA, M. P. S.; REIS, A. Gama de hospedeiros de espécies de Colletotrichum obtidas de chuchuzeiros de regiões brasileiras. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia, 2015. Resumo 143-1 Biblioteca(s): Embrapa Hortaliças. |
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16. | | LIMA, W. G.; MELO FILHO, P. A.; CÂMARA, P. S.; SANTOS, R. C.; CÂMARA, C. A. G.; SILVA, A. M.; GARCIA, A. L.; BEZERRA, C. S. Efeito de óleos vegetais no controle de Colleototrichum gossypii var. cephalosporiodes. In: In: REUNIÃO BRASILEIRA SOBRE CONTROLE BIOLÓGICO DE DOENÇAS DE PLANTAS, 9., 2007, Campinas. Biblioteca(s): Embrapa Algodão. |
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18. | | VIEIRA, W. A. dos S.; LIMA, W. G.; NASCIMENTO, E. S.; MICHEREFF, S. J.; REIS, A.; DOYLE, V. P.; CÂMARA, M. P. S. Thiophanate-methyl resistance and fitness components of colletotrichum musae isolates from banana in Brazil. Plant Disease, Saint Paul, v. 101, p. 1659-1665, Sept. 2017. Biblioteca(s): Embrapa Hortaliças. |
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19. | | LIMA, W. G.; MELO FILHO, P. A.; CÂMARA, P. S.; SANTOS, R. C.; CÂMARA, C. A. G.; SILVA, A. M.; GARCIA, A. L. Uso do óleo de citronela no controle da Ramulose do algodoeiro. In: In: REUNIÃO BRASILEIRA SOBRE CONTROLE BIOLÓGICO DE DOENÇAS DE PLANTAS, 9., 2007, Campinas. Biblioteca(s): Embrapa Algodão. |
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20. | | BRITO NETTO, M. S.; LIMA, W. G.; CORREIA, K. C.; SILVA, C. de F. B. da; THON, M.; MARTINS, R. B.; MILLER, R. N. G.; MICHEREFF, S. J.; CÂMARA, M. P. S. Analysis of phylogeny, distribution, and pathogenicity of Botryosphaeriaceae species associated with gummosis of Anacardium in Brazil, with a new species of Lasiodiplodia. Fungal Biology, v. 121, n. 4, p. 437-451, 2017. Biblioteca(s): Embrapa Agroindústria Tropical. |
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Registros recuperados : 20 | |
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Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
06/12/2021 |
Data da última atualização: |
06/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DIMKIĆ, I.; BHARDWAJ, V.; CARPENTIERI-PIPOLO, V.; KUZMANOVIĆ, N.; DEGRASSI, G. |
Afiliação: |
IVICA DIMKIĆ, Department of Biochemistry and Molecular Biology, University of Belgrade – Faculty of Biology, Belgrade, Serbia; VIBHA BHARDWAJ, Ras Al Khaimah Municipality Department, Director Environment Laboratories, Dubai, United Arab Emirate; VALERIA CARPENTIERI PIPOLO, CNPT; NEMANJA KUZMANOVIĆ, Federal Research Centre for Cultivated Plants (JKI), Institute for Plant Protection in Horticulture and Forests, Julius Ku¨ hn-Institut, Braunschweig, Germany; GIULIANO DEGRASSI, Industrial Biotechnology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina. |
Título: |
The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Plos One, v. 16, n.11, e0259465 Jan. 2021. |
DOI: |
https://doi.org/10.1371/journal. pone.0259465 |
Idioma: |
Inglês |
Conteúdo: |
Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader. MenosCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.... Mostrar Tudo |
Palavras-Chave: |
Curtobacterium sp; Enzyme; GD1. |
Thesaurus NAL: |
Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228529/1/journal.pone.0259465-PLOSONE-2021.pdf
|
Marc: |
LEADER 02369naa a2200229 a 4500 001 2137133 005 2021-12-06 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal. pone.0259465$2DOI 100 1 $aDIMKIĆ, I. 245 $aThe chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.$h[electronic resource] 260 $c2021 520 $aCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader. 650 $aSoybeans 653 $aCurtobacterium sp 653 $aEnzyme 653 $aGD1 700 1 $aBHARDWAJ, V. 700 1 $aCARPENTIERI-PIPOLO, V. 700 1 $aKUZMANOVIĆ, N. 700 1 $aDEGRASSI, G. 773 $tPlos One$gv. 16, n.11, e0259465 Jan. 2021.
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