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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/03/2011 |
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Data da última atualização: |
29/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTUA, J. |
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Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
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Título: |
Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Analytical methods, [S. l.], v. 3, p. 552-556, 2011. |
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DOI: |
10.1039/C0AY00538J |
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Idioma: |
Inglês |
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Conteúdo: |
The citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses. |
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Palavras-Chave: |
Citrus greening disease; Doença do greening citrus; Huanglongbing; Method of fluorescence images; Método de imagens de fluorescência; Plant disease; Statistical method. |
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Thesagro: |
Bactéria; Greening; Método Estatístico. |
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Thesaurus Nal: |
Candidatus Liberibacter. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02525naa a2200337 a 4500
001 1900658
005 2019-01-29
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1039/C0AY00538J$2DOI
100 1 $aPEREIRA, F. M. V.
245 $aFluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection.$h[electronic resource]
260 $c2011
520 $aThe citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses.
650 $aCandidatus Liberibacter
650 $aBactéria
650 $aGreening
650 $aMétodo Estatístico
653 $aCitrus greening disease
653 $aDoença do greening citrus
653 $aHuanglongbing
653 $aMethod of fluorescence images
653 $aMétodo de imagens de fluorescência
653 $aPlant disease
653 $aStatistical method
700 1 $aMILORI, D. M. B. P.
700 1 $aPEREIRA-FILHO, E. R.
700 1 $aVENANCIO, A. L.
700 1 $aRUSSO, M. de S. T.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTUA, J.
773 $tAnalytical methods, [S. l.]$gv. 3, p. 552-556, 2011.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
03/03/2020 |
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Data da última atualização: |
04/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LUDWIG, L. J.; CARPENTIERI-PIPOLO, V.; LOPES, K. B. A.; MINELLA, E.; BELEIA, A. D. P.; GROSSMANN, M. V. |
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Afiliação: |
Leniza Januário Ludwig, Universidade Estadual de Londrina, Londrina-PR, Brazil. E-mail: ludwig.leniza@gmail.com (ORCID: 0000-0002-7549-6026); VALERIA CARPENTIERI PIPOLO, CNPT; Karla Bianca Almeida Lopes, Universidade Estadual de Londrina, Londrina-PR, Brazil. E-mail: karla.bianca.lopes@gmail.com (ORCID: 0000-0002-5111-8709); EUCLYDES MINELLA, CNPT; Adelaide del Pino Beleia, Universidade Estadual de Londrina, Londrina-PR, Brazil. E-mail:beleia@uel.br (ORCID: 0000-0002-8143-3603); Maria Victoria Eiras Grossmann, Universidade Estadual de Londrina, Londrina-PR, Brazil. E-mail:mvgrossmann@gmail.com ((ORCID: 0000-0002-7226-4940. |
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Título: |
Comparison and quality evaluation of hull-less and covered Brazilian barleyfor food industry application. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Ciências Agrárias, v. 14, n. 4, e6942, 2019. |
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DOI: |
10.5039/agraria.v14i4a694 |
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Idioma: |
Inglês |
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Conteúdo: |
Barley has a wide range of end uses. However, the technological characteristics expected from barley present different standards according to the destination of the cereal. Grain β-glucan content is the most important attribute for varieties destined for the food market due to blood glucose and cholesterol-reducing properties. High protein content, test weight, and huller rate may also add value to different end uses. In Brazil, the main destination for barley is malt production; however, not every lot achieves malting standards. To determine the quality of Brazilian barley for food industries, 9 covered barley cultivars and 8 hull-less barley breeding lines were studied. Thousand kernel weight (TKW), hectoliter weight (HW), huller rate (HR), protein, and β-glucan contents were analyzed. The hull-less breeding lines presented higher averages when compared to the covered group, except in protein content. Correlations between ?β-glucan and HW?, ?β-glucan and TKW?, and ?TKW and HW? were positive. On the other hand, ?HW and protein content? and ?β-glucan and protein content? presented a negative correlation. There are bromatological quality differences between Brazilian hull-less breeding lines and covered varieties. Brazilian barley germplasm presents great industrial potential, not only for malt production and animal feed but also for human food applications.Key words:β-glucan; hectoliter weight, Hordeum vulgare; naked barley; protein |
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Palavras-Chave: |
?-glucan; Hectoliter weight; Naked barley; Protein. |
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Thesagro: |
Hordeum Vulgare. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211426/1/CevadaHulless2019.pdf
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Marc: |
LEADER 02262naa a2200253 a 4500
001 2120823
005 2020-03-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5039/agraria.v14i4a694$2DOI
100 1 $aLUDWIG, L. J.
245 $aComparison and quality evaluation of hull-less and covered Brazilian barleyfor food industry application.$h[electronic resource]
260 $c2019
520 $aBarley has a wide range of end uses. However, the technological characteristics expected from barley present different standards according to the destination of the cereal. Grain β-glucan content is the most important attribute for varieties destined for the food market due to blood glucose and cholesterol-reducing properties. High protein content, test weight, and huller rate may also add value to different end uses. In Brazil, the main destination for barley is malt production; however, not every lot achieves malting standards. To determine the quality of Brazilian barley for food industries, 9 covered barley cultivars and 8 hull-less barley breeding lines were studied. Thousand kernel weight (TKW), hectoliter weight (HW), huller rate (HR), protein, and β-glucan contents were analyzed. The hull-less breeding lines presented higher averages when compared to the covered group, except in protein content. Correlations between ?β-glucan and HW?, ?β-glucan and TKW?, and ?TKW and HW? were positive. On the other hand, ?HW and protein content? and ?β-glucan and protein content? presented a negative correlation. There are bromatological quality differences between Brazilian hull-less breeding lines and covered varieties. Brazilian barley germplasm presents great industrial potential, not only for malt production and animal feed but also for human food applications.Key words:β-glucan; hectoliter weight, Hordeum vulgare; naked barley; protein
650 $aHordeum Vulgare
653 $a?-glucan
653 $aHectoliter weight
653 $aNaked barley
653 $aProtein
700 1 $aCARPENTIERI-PIPOLO, V.
700 1 $aLOPES, K. B. A.
700 1 $aMINELLA, E.
700 1 $aBELEIA, A. D. P.
700 1 $aGROSSMANN, M. V.
773 $tRevista Brasileira de Ciências Agrárias$gv. 14, n. 4, e6942, 2019.
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Embrapa Trigo (CNPT) |
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