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Registros recuperados : 134 | |
12. | | BARRUETO CID, L. P.; OLIVA, M. A.; CARDOSO, A. A. Efeito do potencial hídrico sobre a embebição, a respiração e a germinação da leguminosa Cratylia floribunda. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 16, n. 6, p. 883-890, nov./dez. 1981 Título em inglês: Effect of water potential on imbibition, respiration and germination of leguminous Cratylia floribunda. Biblioteca(s): Embrapa Unidades Centrais. |
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13. | | CID, L. P. B.; OLIVA, M. A.; CARDOSO, A. A. Efeito do potencial hídrico sobre a embebição, a respiração e a germinação da leguminosa Cratylia floribunda. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 16, n. 6, p. 883-890, nov./dez. 1981. Título em inglês: Effect of water potential on imbibition, respiration and germination of leguminous Cratylia floribunda. Biblioteca(s): Embrapa Amazônia Ocidental. |
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16. | | VIEIRA, M. C.; CASALI, V. W. D.; CARDOSO, A. A. Avaliacao de quatro clones de mandioquinha-salsa, em dois arranjos de plantas, em Dourados-MS. Horticultura Brasileira, Brasilia, v.13, n.1, p.120, maio 1995. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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17. | | SEDIYAMA, T.; CARDOSO, A. A.; VIEIRA, G.; ANDRADE, D. de. Taxa de hibridação natural na soja, em Viçosa e em Capinópolis, Minas Gerais. Revista Ceres, Viçosa, v. 17, n. 94, p. 329-331, out./dez. 1970. Biblioteca(s): Embrapa Soja. |
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19. | | VIEIRA, M. C.; CASALI, V. W. D.; CARDOSO, A. A.; MOSQUIM, P. R. Competicao de quatro clones de mandioquinha-salsa, em Dourados-MS. Horticultura Brasileira, Brasilia, v.13, n.1, p.121, maio 1995. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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Registros recuperados : 134 | |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
14/12/2016 |
Data da última atualização: |
03/10/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CARDOSO, A. A.; ANDRAUS, M. de P.; BORBA, T. C. de O.; MARTIN-DIDONET, C. C. G.; FERREIRA, E. P. de B. |
Afiliação: |
ALINE ASSIS CARDOSO, UFG; MICHEL DE PAULA ANDRAUS, UFG; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS, Anápolis-GO; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF. |
Título: |
Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 48, n. 1, p. 43-50, jan./mar. 2017. |
ISSN: |
1678-4405 |
DOI: |
10.1016/j.bjm.2016.09.002 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments. MenosThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean i... Mostrar Tudo |
Palavras-Chave: |
Carbon source use; Symbiotic efficiency; Toleance to salinity and temperature. |
Thesagro: |
Feijão; Phaseolus vulgaris; Rhizobium; Simbiose. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156257/1/CNPAF-2017-bjm.pdf
|
Marc: |
LEADER 02432naa a2200277 a 4500 001 2058672 005 2017-10-03 008 2017 bl uuuu u00u1 u #d 022 $a1678-4405 024 7 $a10.1016/j.bjm.2016.09.002$2DOI 100 1 $aCARDOSO, A. A. 245 $aCharacterization of rhizobia isolates obtained from nodules of wild genotypes of common bean.$h[electronic resource] 260 $c2017 520 $aThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments. 650 $aFeijão 650 $aPhaseolus vulgaris 650 $aRhizobium 650 $aSimbiose 653 $aCarbon source use 653 $aSymbiotic efficiency 653 $aToleance to salinity and temperature 700 1 $aANDRAUS, M. de P. 700 1 $aBORBA, T. C. de O. 700 1 $aMARTIN-DIDONET, C. C. G. 700 1 $aFERREIRA, E. P. de B. 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 48, n. 1, p. 43-50, jan./mar. 2017.
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