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| Registros recuperados : 147 | |
| 5. |  | TUPINAMBA, D.; CANTAO, M. E.; COSTA, O. Y.; BERGMANN, J. C.; KYAW, C.; QUIRINO, B. F. Impact of oil palm fatal yellowing on Amazon soil archaeal community assessed by high-throughput sequencing. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Resumo T141| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | SCHAEFER, R.; GAVA, D.; NELSON, M. I.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Genetic characterization of influenza viruses circulating within Brazilian swine between 2009 and 2016. In: BRAZILIAN CONGRESS OF VIROLOGY, 27.; MERCOSUR MEETING OF VIROLOGY,11., 2016, Pirenópolis. Abstracts: posters: oral presentation. Brasília, DF: SBV, 2016. Publicado na Virus Reviews and Research, Brasília, n. 20, v. 2, p. 22-23, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | SCHAEFER, R.; CANTAO, M. E.; ROCHA, C. S.; GAVA, D.; ZANELLA, J. R. C. Full genome sequencing of a human-like H1N2 swine influenza virus. In: BRAZILIAN CONGRESS OF VIROLOGY, 23.; MERCOSUR MEETING OF VIROLOGY, 7., 2012, Foz do Iguaçu. Virus and Research? Foz do Iguaçu: SBV, 2012. p. 472-473. Journal of the Brazilian Society for Virology, v. 17, n. 2, suppl. 1, 2012. Projeto/Plano de Ação: 02.11.01.006.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 13. | | SILVA, M. L. B. da; CANTAO, M. E.; MEZZARI, M. P.; MA, J.; NOSSA, C. W. Assessment of bacterial and archaeal community structure in swine wastewater treatment processes. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | MEZZARI, M. P.; SILVA, M. L. B. da; CANTAO, M. E.; MA, J.; NOSSA, C. W. Insights of putative pathogens reduction from swine wastewater treatment processes through 16s rDNA pyrosequencing analyses. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 15. | | NELSON, M. I.; SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Influenza A viruses of human origin in swine, Brazil. Emerging Infectious Diseases, v. 21, n. 8, p. 1339 - 1347, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 16. | | ZANELLA, J. R. C.; SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; COLDEBELLA, A. Influenza A virus infection in Brazilian swine herds following the introduction of pandemic 2009 H1N1. Veterinary Microbiology, v. 180, n. 1-2, p. 118-122, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 17. | | SCHAEFER, R.; MORES, N.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Long-term circulation of influenza A viruses in swine in Brazil. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 53-54, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 20. | | PEIXOTO, J. de O.; IBELLI, A. M. G.; CANTAO, M. E.; LEDUR, M. C. Condronecrose bacteriana com osteomielite (BCO): um problema subestimado na avicultura de corte. Guia Gessulli da Avicultura e Suinocultura Industrial, Itu, ed. 266, ano 38, n. 5, p. 24-30, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
11/04/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
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Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
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Thesagro: |
Frango de Corte; Salmonelose. |
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Thesaurus NAL: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02834naa a2200337 a 4500
001 2163526
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI
100 1 $aVAZ, C. S. L.
245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource]
260 $c2024
520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.
650 $aBacteriophages
650 $aPhage therapy
650 $aPoultry
650 $aSalmonellosis
650 $aFrango de Corte
650 $aSalmonelose
653 $aAnálise genômica
653 $aBacteriófago
653 $aFoodborne pathogen
653 $aGenomic analysis
653 $aPatógeno de origem alimentar
653 $aTerapia fágica
700 1 $aFONSECA, F. M. da
700 1 $aRECH, D. V.
700 1 $aMORES, M. A. Z.
700 1 $aCOLDEBELLA, A.
700 1 $aCANTAO, M. E.
773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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