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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, I. O. e; VASCONCELOS, R. B.; CAETANO, J. V. O.; GULART, L. V. M.; CAMARGO, L. S. de A.; BÁO, S. N.; SILVA, A. A. M. R. e. |
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Afiliação: |
I. OLIVEIRA E SILVA, UNB; R. B. VASCONCELOS, UNB; J. V. O. CAETANO, UNB; L. V. M. GULART, UNB; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; S. N. BÁO, UNB; A. A. M. ROSA E SILVA, UNB. |
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Título: |
Induction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 75, n. 6, p. 1115-1124, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2010.11.021 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM. MenosThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additio... Mostrar Tudo |
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Palavras-Chave: |
Chemically defined medium; Cytoplasmic maturation; Nuclear maturation. |
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Thesaurus Nal: |
Oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02902naa a2200253 a 4500
001 1909108
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2010.11.021$2DOI
100 1 $aSILVA, I. O. e
245 $aInduction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions.$h[electronic resource]
260 $c2011
520 $aThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM.
650 $aOocytes
653 $aChemically defined medium
653 $aCytoplasmic maturation
653 $aNuclear maturation
700 1 $aVASCONCELOS, R. B.
700 1 $aCAETANO, J. V. O.
700 1 $aGULART, L. V. M.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBÁO, S. N.
700 1 $aSILVA, A. A. M. R. e
773 $tTheriogenology$gv. 75, n. 6, p. 1115-1124, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
22/11/2012 |
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Data da última atualização: |
22/11/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SETOTAW, T. A.; RIBEIRO, A. P.; FERREIRA, J. L.; PASQUAL, M.; PIO, L.; FRANÇA, D. V. C.; BREVES, S. S.; GENEROSO, A. L.; CANÇADO, G. M. A. |
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Afiliação: |
T. A. SETOTAW, EPAMIG; A. P. RIBEIRO, UFL; JULIANO LINO FERREIRA, CPPSUL; M. PASQUAL, UFL; L. PIO, UFL; D. V. C. FRANÇA, EPAMIG; S. S. BREVES, EPAMIG; A. L. GENEROSO, EPAMIG; G. M. A. CANÇADO, EPAMIG. |
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Título: |
Detecting somaclonal variation during micropropagation of vine rootstocks using SSR molecular marker and flow cytometery analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçu: SBG, 2012. |
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Páginas: |
p. 44. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo. |
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Thesagro: |
Marcador Molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70623/1/Juliano-resumo.pdf
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Marc: |
LEADER 00770nam a2200229 a 4500
001 1940305
005 2012-11-22
008 2012 bl uuuu u00u1 u #d
100 1 $aSETOTAW, T. A.
245 $aDetecting somaclonal variation during micropropagation of vine rootstocks using SSR molecular marker and flow cytometery analysis.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçu: SBG$c2012
300 $ap. 44.
520 $aResumo.
650 $aMarcador Molecular
700 1 $aRIBEIRO, A. P.
700 1 $aFERREIRA, J. L.
700 1 $aPASQUAL, M.
700 1 $aPIO, L.
700 1 $aFRANÇA, D. V. C.
700 1 $aBREVES, S. S.
700 1 $aGENEROSO, A. L.
700 1 $aCANÇADO, G. M. A.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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