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| 145. |  | SERENO, J. R. B.; NASCIMENTO, R. L.; CONGRO, C.; ROESE, A. D. Ponto de encontro sobre ciência e cultura no Pantanal. In: ENCONTRO DE EDUCADORAS AMBIENTAIS DA REDE MATO-GROSSENSE DE EDUCAÇÃO AMBIENTAL, 3.; ENCONTRO DA REDE AGUAPÉ DE EDUCAÇÃO AMBIENTAL, 1.; SEMINÁRIO DE EDUCAÇÃO AMBIENTAL DAS CIDADES PANTANEIRAS, 4.; SEMINÁRIO DA CARTA DA TERRA, 3.; FÓRUM REGIONAL DA REDE BRASILEIRA DE EDUCAÇÃO AMBIENTAL, 1., 2003, Cuiabá. Fome zero da educação ambiental: resumo de trabalhos. Cuiabá: REMTEA: AGUAPÉ: MUPAN: C. TERRA: REBEA, 2003. p.79.| Biblioteca(s): Embrapa Pantanal. |
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| 148. | | POTO, A.; PEINADO, B.; SERENO, J. R. B.; CABELLO, A.; BARBA, C. Programas de conservacion ex situ en Espana. Porci, Madrid, n.60, p.49-59, nov., 2000.| Biblioteca(s): Embrapa Pantanal. |
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| 149. | | SERENO, J. R. B.; BARROS, G. de; HERNANDEZ, I.; SOUZA, J. C. de; STORCK, D. E. Proposta de estação de monta para bovinos na sub-região da Nhecolândia, Pantanal. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 43., 2006, João Pessoa. Anais... João Pessoa: SBZ; UFPB, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 156. | | BENAVENTE, M.; SILVEIRA, C.; BARBA, C.; CAMACHO, M. E.; SERENO, J. R. B.; DELGADO, J. V. Tecnologia de la conservacion: II. programas "ex situ". Canis et Felis, Madrid, n.47, p.61-68, oct., 2000.| Biblioteca(s): Embrapa Pantanal. |
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| 157. | | BENAVENTE, M.; SILVEIRA, C.; BARBA, C.; CAMACHO, M. E.; SERENO, J. R. B.; DELGADO, J. V. Tecnología de la conservación II: Programas "ex situ". Separata de: Canis et Felis, Madirid, n. 47, p. 61-68, 2000.| Biblioteca(s): Embrapa Pantanal. |
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| 160. | | SERENO, J. R. B.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A.; DELGADO, J. V. Training of Iberian pigs on mounting ONTO a DUMMY at an artificial insemination programme. In: WORLD CONFERENCE ON ANIMAL PRODUCTION, 9.; REUNIAO DA ASSOCIACAO LATINOAMERICANA DE PRODUCAO ANIMAL, 18., 2003, Porto Alegre. Abstracts. Porto Alegre: [s.n.], 2003. nao paginado. CD-ROM.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 408 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/12/2016 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CAMPOS-JUNIOR, P. H. A.; ALVES, T. J. M.; DIAS, M. T.; ASSUNÇAO, C. M.; MUNK, M.; MATTOS, M. S.; KRAEMER, L. R.; ALMEIDA, B. G.; RUSSO, R. C.; BARCELOS, L.; CAMARGO, L. S. de A.; VIANA, J. H. M. |
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Afiliação: |
PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, UFSJ; THALYS JAIR MELO ALVES, UFSJ; MARCO TULIO DIAS, UFSJ; CAROLINA MARINHO ASSUNÇAO, UFJF; MICHELE MUNK, UFJF; MATHEUS SILVÉRIO MATTOS, UFMG; LUCAS ROCHA KRAEMER, UFMG; BRÍGIDA GOMES ALMEIDA, UFMG; REMO CASTRO RUSSO, UFMG; LUCÍOLA BARCELOS, UFMG; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 6, e0158109, 2016. |
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DOI: |
10.1371/journal. pone.0158109 |
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Idioma: |
Inglês |
|
Conteúdo: |
Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes. MenosAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. H... Mostrar Tudo |
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Palavras-Chave: |
Bovine ovarian grafts; Ovarian xenografts. |
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Thesaurus NAL: |
oocytes. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154925/1/Cnpgl-2016-PlosOne-Ovarian.PDF
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|
Marc: |
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100 1 $aCAMPOS-JUNIOR, P. H. A.
245 $aOvarian Grafts 10 days after Xenotransplantation$bfolliculogenesis and recovery of viable oocytes.$h[electronic resource]
260 $c2016
520 $aAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.
650 $aoocytes
653 $aBovine ovarian grafts
653 $aOvarian xenografts
700 1 $aALVES, T. J. M.
700 1 $aDIAS, M. T.
700 1 $aASSUNÇAO, C. M.
700 1 $aMUNK, M.
700 1 $aMATTOS, M. S.
700 1 $aKRAEMER, L. R.
700 1 $aALMEIDA, B. G.
700 1 $aRUSSO, R. C.
700 1 $aBARCELOS, L.
700 1 $aCAMARGO, L. S. de A.
700 1 $aVIANA, J. H. M.
773 $tPlos One$gv. 11, n. 6, e0158109, 2016.
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