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| Registros recuperados : 243 | |
| 61. |  | SOUSA, A. C. B.; JANK, L.; CAMPOS, T.; SFORÇA, D. A.; JUNGMANN, L.; SOUZA, A. P. Caracterização molecular do banco de germoplasma de Panicum maximum Jacq. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE,5., 2009, Campo Grande, MS. [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 62. | | FERREIRA, J. A.; SILVA, H. F.; ASSIS, G. M. L. de; CAMPOS, T. de. Caracterização da diversidade genética de genitores de amendoim forrageiro por meio de marcadores microssatélites. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 4 p. 1 CD-ROM.| Biblioteca(s): Embrapa Acre. |
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| 63. | | SIVIERO, A.; BERGO, C. L.; SILVA, L. M. da; KLEIN, M. A.; CAMPOS, T. de. Caracterização de etnovariedades de mandioca utilizadas na produção de farinha de Cruzeiro do Sul. In: CONGRESSO BRASILEIRO DE MANDIOCA, 17.; CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2., 2018, Belém, PA. Anais... Belém, PA: SBM, 2018. p. 197-200.| Biblioteca(s): Embrapa Acre. |
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| 65. | | LOPES, L. R.; OLIVEIRA, F. de A. de; GHEYI, H. R.; CAMPOS, T. G. da S. Desenvolvimento do algodão herbáceo (Gossypium hirsutum latifoliuns) irrigado num solo sódico em fase de recuperação. IN: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 25., 1995, Viçosa, MG.Resumos expandidas. Viçosa: UFV, 1995. v.4, p.2269-2270.| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 243 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
08/10/2015 |
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Data da última atualização: |
03/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CAMPOS, T. de; PISSETTI, C.; WERLANG, G. O.; LOPES, G. V.; KICH, J. D.; CARDOSO, M. R. de I. |
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Afiliação: |
THAIS DE CAMPOS, UFRGS/Faculdade de Veterinária; CAROLINE PISSETTI, UFRGS/Faculdade de Veterinária; GABRIELA OROSCO WERLANG, UFRGS/Faculdade de Veterinária; GRACIELA VOLZ LOPES, UFRGS/Faculdade de Veterinária; JALUSA DEON KICH, CNPSA; MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS/Faculdade de Veterinária. |
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Título: |
Antimicrobial resistance of enterococcus isolated from pre-chill swine carcasses. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, n. 1259, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: As a result of the extensive use of antimicrobials in agriculture, animals have been implicated as possible reservoirs of resistant strains of bacteria. Enterococci are members of the normal flora of the gastrointestinal tract of human and animals. Because of their ubiquity, enterococci have been introduced in programs to evaluate the hazard of transmission of resistant bacterial strains through the food chain. The aim of this study was to assess the antimicrobial resistance profile of Enterococcus isolated from swine carcasses at the pre-chill step of processing. Material, Methods & Results: Pig carcasses were sampled at three commercial slaughterhouses (A, B and C). On each of two sampling occasions swabs of 100 cm2 areas were taken from each ham, back, belly and jowl of a total of 14 pre-chill carcasses. Enterococci were isolated and counted in KF Streptococcus Agar, and typical colonies were confirmed by PCR assay targeting the tuf gene. Enterococcus isolates were tested for susceptibility to nine different antimicrobial agents by agar disc diffusion. From a total of 252 carcasses sampled, 240 (95.2%) presented presumptive colonies of Enterococcus in counts ranging from 0.02 log CFU.cm-2 to 2.9 log CFU.cm-2. All isolates were confirmed as belonging to the genus Enterococcus, and the great majority was identified as E. faecalis (218/240; 90.83%). Half (125/240; 52.1%) of the Enterococcus isolates were susceptible to all tested antimicrobials. No resistance to ampicillin, vancomycin or teicoplanin was found. The most frequent resistance was to tetracycline (42.5%), followed by erythromycin (26.7%), high level (HLR) streptomycin (20.4%), ciprofloxacin (13.8%), chloramphenicol (12.1%) and HLR-gentamicin (10.4%). Among the 115 resistant Enterococcus isolates, 55 (47.8%) were multi-resistant, and the distribution of the most common profiles was related to the slaughterhouse from which the isolate originated. Discussion: Although foodborne enterococci have not yet been clearly involved in direct clinical infection, antimicrobial-resistant isolates from food can be a reservoir of resistance genes. Therefore, the occurrence of resistant isolates to HLR-streptomycin, HLR-gentamicin, tetracycline, erythromycin and ciprofloxacin in swine carcasses may present a risk of spreading enterococci strains that are resistant to treatment. Several drugs of these groups are used in feed for prophylaxis and treatment of respiratory and enteric diseases in pigs and may thus be exerting a high selective pressure in the intestinal microbiota. The sampled slaughterhouses in this study were supplied by production chains that belong to different agribusiness companies. These companies run contracts with farmers which usually specify a common management protocol, including prophylactic and therapeutic administration of antimicrobial drugs. The selective pressure of antimicrobial usage may also explain the significant difference in the frequency of resistance to most tested agents among the slaughterhouses. It was concluded that although Enterococcus isolates from pre-chill swine carcasses presented a low to moderate frequency of resistance against most antimicrobials used in human treatment, isolates displaying resistance to the aminoglycosides and macrolides classes may present a hazard. The multi-resistance patterns were highly associated with the origin of the isolates and may indicate the extent of antimicrobial use on farm. MenosBackground: As a result of the extensive use of antimicrobials in agriculture, animals have been implicated as possible reservoirs of resistant strains of bacteria. Enterococci are members of the normal flora of the gastrointestinal tract of human and animals. Because of their ubiquity, enterococci have been introduced in programs to evaluate the hazard of transmission of resistant bacterial strains through the food chain. The aim of this study was to assess the antimicrobial resistance profile of Enterococcus isolated from swine carcasses at the pre-chill step of processing. Material, Methods & Results: Pig carcasses were sampled at three commercial slaughterhouses (A, B and C). On each of two sampling occasions swabs of 100 cm2 areas were taken from each ham, back, belly and jowl of a total of 14 pre-chill carcasses. Enterococci were isolated and counted in KF Streptococcus Agar, and typical colonies were confirmed by PCR assay targeting the tuf gene. Enterococcus isolates were tested for susceptibility to nine different antimicrobial agents by agar disc diffusion. From a total of 252 carcasses sampled, 240 (95.2%) presented presumptive colonies of Enterococcus in counts ranging from 0.02 log CFU.cm-2 to 2.9 log CFU.cm-2. All isolates were confirmed as belonging to the genus Enterococcus, and the great majority was identified as E. faecalis (218/240; 90.83%). Half (125/240; 52.1%) of the Enterococcus isolates were susceptible to all tested antimicrobials. No resistance to ... Mostrar Tudo |
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Palavras-Chave: |
Enterococcus porcines. |
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Thesagro: |
Antibiótico; Bactéria; Carcaça; Microbiologia; Suíno. |
|
Thesaurus NAL: |
Antibiotic resistance; Bacteriology; Pig carcasses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04278naa a2200289 a 4500
001 2026050
005 2016-06-03
008 2015 bl uuuu u00u1 u #d
100 1 $aCAMPOS, T. de
245 $aAntimicrobial resistance of enterococcus isolated from pre-chill swine carcasses.$h[electronic resource]
260 $c2015
520 $aBackground: As a result of the extensive use of antimicrobials in agriculture, animals have been implicated as possible reservoirs of resistant strains of bacteria. Enterococci are members of the normal flora of the gastrointestinal tract of human and animals. Because of their ubiquity, enterococci have been introduced in programs to evaluate the hazard of transmission of resistant bacterial strains through the food chain. The aim of this study was to assess the antimicrobial resistance profile of Enterococcus isolated from swine carcasses at the pre-chill step of processing. Material, Methods & Results: Pig carcasses were sampled at three commercial slaughterhouses (A, B and C). On each of two sampling occasions swabs of 100 cm2 areas were taken from each ham, back, belly and jowl of a total of 14 pre-chill carcasses. Enterococci were isolated and counted in KF Streptococcus Agar, and typical colonies were confirmed by PCR assay targeting the tuf gene. Enterococcus isolates were tested for susceptibility to nine different antimicrobial agents by agar disc diffusion. From a total of 252 carcasses sampled, 240 (95.2%) presented presumptive colonies of Enterococcus in counts ranging from 0.02 log CFU.cm-2 to 2.9 log CFU.cm-2. All isolates were confirmed as belonging to the genus Enterococcus, and the great majority was identified as E. faecalis (218/240; 90.83%). Half (125/240; 52.1%) of the Enterococcus isolates were susceptible to all tested antimicrobials. No resistance to ampicillin, vancomycin or teicoplanin was found. The most frequent resistance was to tetracycline (42.5%), followed by erythromycin (26.7%), high level (HLR) streptomycin (20.4%), ciprofloxacin (13.8%), chloramphenicol (12.1%) and HLR-gentamicin (10.4%). Among the 115 resistant Enterococcus isolates, 55 (47.8%) were multi-resistant, and the distribution of the most common profiles was related to the slaughterhouse from which the isolate originated. Discussion: Although foodborne enterococci have not yet been clearly involved in direct clinical infection, antimicrobial-resistant isolates from food can be a reservoir of resistance genes. Therefore, the occurrence of resistant isolates to HLR-streptomycin, HLR-gentamicin, tetracycline, erythromycin and ciprofloxacin in swine carcasses may present a risk of spreading enterococci strains that are resistant to treatment. Several drugs of these groups are used in feed for prophylaxis and treatment of respiratory and enteric diseases in pigs and may thus be exerting a high selective pressure in the intestinal microbiota. The sampled slaughterhouses in this study were supplied by production chains that belong to different agribusiness companies. These companies run contracts with farmers which usually specify a common management protocol, including prophylactic and therapeutic administration of antimicrobial drugs. The selective pressure of antimicrobial usage may also explain the significant difference in the frequency of resistance to most tested agents among the slaughterhouses. It was concluded that although Enterococcus isolates from pre-chill swine carcasses presented a low to moderate frequency of resistance against most antimicrobials used in human treatment, isolates displaying resistance to the aminoglycosides and macrolides classes may present a hazard. The multi-resistance patterns were highly associated with the origin of the isolates and may indicate the extent of antimicrobial use on farm.
650 $aAntibiotic resistance
650 $aBacteriology
650 $aPig carcasses
650 $aAntibiótico
650 $aBactéria
650 $aCarcaça
650 $aMicrobiologia
650 $aSuíno
653 $aEnterococcus porcines
700 1 $aPISSETTI, C.
700 1 $aWERLANG, G. O.
700 1 $aLOPES, G. V.
700 1 $aKICH, J. D.
700 1 $aCARDOSO, M. R. de I.
773 $tActa Scientiae Veterinariae$gv. 43, n. 1259, 2015.
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