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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/04/2005 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ROMANOWSKI, V.; ARANA, E. I.; MCCARTHY. C. B.; BIEDMA, M. E.; SCIOCCO-CAP, A.; GOLDBERG, A. V.; GHIRINGHELLI, P. D.; PINEDO, F. J. R.; MOSCARDI, F.; RIBEIRO, B. M. |
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Afiliação: |
CNPSO. |
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Título: |
Advances towards improving the insecticidal properties of AgMNPV. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003. |
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Páginas: |
p. 32-33. |
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Idioma: |
Inglês |
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Conteúdo: |
Anticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansion in order to control simultaneous lepidopteran pests would certainly increase the appeal of AgMNPV to soybean growers. Controlled field experiments will address th applicability of these and other genetically improved AgMNPVs in large scale programs. The traceability of the recombinants will be facilitated by the insertion of reporter genes. MenosAnticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansi... Mostrar Tudo |
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Thesagro: |
Baculovirus; Controle Biológico; Praga de Planta; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02707nam a2200289 a 4500
001 1467932
005 2020-02-27
008 2003 bl uuuu u00u1 u #d
100 1 $aROMANOWSKI, V.
245 $aAdvances towards improving the insecticidal properties of AgMNPV.$h[electronic resource]
260 $aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington$c2003
300 $ap. 32-33.
520 $aAnticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansion in order to control simultaneous lepidopteran pests would certainly increase the appeal of AgMNPV to soybean growers. Controlled field experiments will address th applicability of these and other genetically improved AgMNPVs in large scale programs. The traceability of the recombinants will be facilitated by the insertion of reporter genes.
650 $aSoybeans
650 $aBaculovirus
650 $aControle Biológico
650 $aPraga de Planta
650 $aSoja
700 1 $aARANA, E. I.
700 1 $aMCCARTHY. C. B.
700 1 $aBIEDMA, M. E.
700 1 $aSCIOCCO-CAP, A.
700 1 $aGOLDBERG, A. V.
700 1 $aGHIRINGHELLI, P. D.
700 1 $aPINEDO, F. J. R.
700 1 $aMOSCARDI, F.
700 1 $aRIBEIRO, B. M.
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