| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
05/10/2004 |
|
Data da última atualização: |
15/03/2011 |
|
Autoria: |
TEIXEIRA-CABRAL, T. A.; PENNA, J. C. V.; GOULART, L. R. |
|
Título: |
A comparative analysis of genetic distances among 24 upland cotton genotypes using RAPD markers and phenotypic characters. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 1, p. 85-95, Mar. 2002. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The knowledge of genetic distances among individuals or populations in plant breeding programs can be used to complement phenotypic information and to help the selection of individuals to breed towards segregating populations with high variability. The objectives of this study were to identify primers that produce polymorphisms among cotton (Gossypium hirsutum L.) genotypes, to compare the effect of primer length in the generation of polymorphisms, to determine genetic distances among 24 cotton genotypes from eight countries using the RAPD technique and compare them with results obtained throught phenetic analysis of the genotypes based on 52 qualitative and quantitative characteristics using numerical taxonomy. The 24 genotypes were evaluated by the RAPD technique utilizing short primers and combinations of long primers. RAPDs were scored as presence (1) or absence {0} of prominent bands reproducible in duplicated PCR reactions. These data were subjected to analyses to produce a dissimilarity matrix based on Simple, Nei and Li's and Jaccard's Distances. UPGMA was utilized to create dendrograms based on the genetic distance matrices. Short primers produced polymorphic bands that were efficient to discriminate among genotypes, while long primers couldn"t detect differences among the genotypes. Comparing the three methods used for estimation of genetic distances, the method that better dealt with distances obtained by using phenotypic character analyses was the Simple Distance Method. The correlation among the results of this method with Jaccard's and Nei and Li's Distances were 93 % and 91 %, respectively. The maximum simple distance found between pairs of genotypes based in the polymorphisms produced by short primers was 18%, which possibly indicates a narrower genetic basis than in the phenotypic analysis. The correlation among phenetic analysis and RAPD based on short primers was moderate and negative, tough significant [r=0,43). MenosThe knowledge of genetic distances among individuals or populations in plant breeding programs can be used to complement phenotypic information and to help the selection of individuals to breed towards segregating populations with high variability. The objectives of this study were to identify primers that produce polymorphisms among cotton (Gossypium hirsutum L.) genotypes, to compare the effect of primer length in the generation of polymorphisms, to determine genetic distances among 24 cotton genotypes from eight countries using the RAPD technique and compare them with results obtained throught phenetic analysis of the genotypes based on 52 qualitative and quantitative characteristics using numerical taxonomy. The 24 genotypes were evaluated by the RAPD technique utilizing short primers and combinations of long primers. RAPDs were scored as presence (1) or absence {0} of prominent bands reproducible in duplicated PCR reactions. These data were subjected to analyses to produce a dissimilarity matrix based on Simple, Nei and Li's and Jaccard's Distances. UPGMA was utilized to create dendrograms based on the genetic distance matrices. Short primers produced polymorphic bands that were efficient to discriminate among genotypes, while long primers couldn"t detect differences among the genotypes. Comparing the three methods used for estimation of genetic distances, the method that better dealt with distances obtained by using phenotypic character analyses was the Simple Distan... Mostrar Tudo |
|
Palavras-Chave: |
Genetic distances; Melhoramento genético; RAPD. |
|
Thesagro: |
Algodão; Gossypium Hirsutum. |
|
Thesaurus Nal: |
cotton. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02645naa a2200217 a 4500
001 1212532
005 2011-03-15
008 2002 bl uuuu u00u1 u #d
100 1 $aTEIXEIRA-CABRAL, T. A.
245 $aA comparative analysis of genetic distances among 24 upland cotton genotypes using RAPD markers and phenotypic characters.
260 $c2002
520 $aThe knowledge of genetic distances among individuals or populations in plant breeding programs can be used to complement phenotypic information and to help the selection of individuals to breed towards segregating populations with high variability. The objectives of this study were to identify primers that produce polymorphisms among cotton (Gossypium hirsutum L.) genotypes, to compare the effect of primer length in the generation of polymorphisms, to determine genetic distances among 24 cotton genotypes from eight countries using the RAPD technique and compare them with results obtained throught phenetic analysis of the genotypes based on 52 qualitative and quantitative characteristics using numerical taxonomy. The 24 genotypes were evaluated by the RAPD technique utilizing short primers and combinations of long primers. RAPDs were scored as presence (1) or absence {0} of prominent bands reproducible in duplicated PCR reactions. These data were subjected to analyses to produce a dissimilarity matrix based on Simple, Nei and Li's and Jaccard's Distances. UPGMA was utilized to create dendrograms based on the genetic distance matrices. Short primers produced polymorphic bands that were efficient to discriminate among genotypes, while long primers couldn"t detect differences among the genotypes. Comparing the three methods used for estimation of genetic distances, the method that better dealt with distances obtained by using phenotypic character analyses was the Simple Distance Method. The correlation among the results of this method with Jaccard's and Nei and Li's Distances were 93 % and 91 %, respectively. The maximum simple distance found between pairs of genotypes based in the polymorphisms produced by short primers was 18%, which possibly indicates a narrower genetic basis than in the phenotypic analysis. The correlation among phenetic analysis and RAPD based on short primers was moderate and negative, tough significant [r=0,43).
650 $acotton
650 $aAlgodão
650 $aGossypium Hirsutum
653 $aGenetic distances
653 $aMelhoramento genético
653 $aRAPD
700 1 $aPENNA, J. C. V.
700 1 $aGOULART, L. R.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 2, n. 1, p. 85-95, Mar. 2002.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
24/03/2011 |
|
Data da última atualização: |
25/03/2024 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
PEREIRA, M. M.; SERAPIÃO, R. V.; QUINTÃO, C. R. C.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. |
|
Afiliação: |
UFJF; RAQUEL V. SERAPIÃO, PESAGRO; PESAGRO; LILIAN TAMY IGUMA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
|
Título: |
Apoptose em embriões bovinos produzidos in vitro utilizando oócitos maturados em meio livre de soro e baixa tensão de oxigênio. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 38, p. 383, 2010. |
|
Idioma: |
Português |
|
Notas: |
Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010. |
|
Thesagro: |
Embrião. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/882464/1/Apoptose-em-embrioes-bovinos.pdf
|
|
Marc: |
LEADER 00716nam a2200181 a 4500
001 1882464
005 2024-03-25
008 2010 bl uuuu u00u1 u #d
100 1 $aPEREIRA, M. M.
245 $aApoptose em embriões bovinos produzidos in vitro utilizando oócitos maturados em meio livre de soro e baixa tensão de oxigênio.$h[electronic resource]
260 $aActa Scientiae Veterinariae, v. 38, p. 383$c2010
500 $aEdição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.
650 $aEmbrião
700 1 $aSERAPIÃO, R. V.
700 1 $aQUINTÃO, C. R. C.
700 1 $aIGUMA, L. T.
700 1 $aVIANA, J. H. M.
700 1 $aCAMARGO, L. S. de A.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
