| |
|
|
| Registros recuperados : 2 | |
| 1. |  | CAMARÇO, R. F. E. A.; NASCIMENTO, A. K. Q. do; ANDRADE, E. C. de; LIMA, J. A. A. Biological, serological and molecular comparison between isolates of Cowpea severe mosaic virus. Tropical Plant Pathology, Brasília, DF, v. 34, n. 4, p. 239-244, jul./ago. 2009.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|   |
| 2. | | LIMA, J. A. A.; NASCIMENTO, A. K. Q.; SILVA, G. S.; CAMARÇO, R. F. E. A.; GONÇALVES, M. F. B. Crotalaria paulinea, novo hospedeiro natural do vírus do mosaico severo do caupi. Fitopatologia Brasileira, Brasília, v. 30, n. 4, p. 429-433, jul./ago. 2005.| Biblioteca(s): Embrapa Hortaliças. |
|  |
| Registros recuperados : 2 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
22/02/2010 |
|
Data da última atualização: |
20/09/2010 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 2 |
|
Autoria: |
CAMARÇO, R. F. E. A.; NASCIMENTO, A. K. Q. do; ANDRADE, E. C. de; LIMA, J. A. A. |
|
Afiliação: |
Rosa Felicia E. Araújo Camarço, UFC; Aline K. Queiroz do Nascimento, UFC; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; José Albersio A. Lima, UFC. |
|
Título: |
Biological, serological and molecular comparison between isolates of Cowpea severe mosaic virus. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 34, n. 4, p. 239-244, jul./ago. 2009. |
|
ISSN: |
1982-5676 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cowpea, Vigna unguiculata, can be affected by several diseases, and those caused by viruses are considered of great importance. Cowpea severe mosaic virus (CPSMV), which belongs to the family Comoviridae, genus Comovirus, stands out for its severity and degree of incidence. Its genetic variability can give rise to different strains around the world, including in Brazil. The objective of the present research was to establish a biological, serological and molecular comparison between five CPSMV isolates obtained from naturally infected plants in different States of the Northeast of Brazil. Isolates were obtained from cowpea: CPSMV-AL (State of Alagoas), CPSMV- CE (Ceará); CPSMV-MC (Piauí), CPSMV-PE (Pernambuco) and an isolate obtained from Crotalaria paulinea, in the State of Maranhão - CPSMV-CROT. A host range study evidenced biological differences among the virus isolates, especially in cowpea genotypes. The isolates were showed to be serologically and molecularly related by polymerase chain reaction – PCR, using degenerated primers which amplified two conserved regions in the coat protein and in the replicase genes. Cloning and sequence of CPSMV-PE made possible its comparison with other CPSMV isolates and other virus species from the genus Comovirus.
Keywords: Vigna unguiculata, Comovirus, CPSMV, molecular differentiation. |
|
Palavras-Chave: |
CPSMV; Molecular differentiation. |
|
Thesagro: |
Vigna Unguiculata. |
|
Thesaurus NAL: |
Comovirus. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02015naa a2200217 a 4500
001 1658409
005 2010-09-20
008 2009 bl uuuu u00u1 u #d
022 $a1982-5676
100 1 $aCAMARÇO, R. F. E. A.
245 $aBiological, serological and molecular comparison between isolates of Cowpea severe mosaic virus.
260 $c2009
520 $aCowpea, Vigna unguiculata, can be affected by several diseases, and those caused by viruses are considered of great importance. Cowpea severe mosaic virus (CPSMV), which belongs to the family Comoviridae, genus Comovirus, stands out for its severity and degree of incidence. Its genetic variability can give rise to different strains around the world, including in Brazil. The objective of the present research was to establish a biological, serological and molecular comparison between five CPSMV isolates obtained from naturally infected plants in different States of the Northeast of Brazil. Isolates were obtained from cowpea: CPSMV-AL (State of Alagoas), CPSMV- CE (Ceará); CPSMV-MC (Piauí), CPSMV-PE (Pernambuco) and an isolate obtained from Crotalaria paulinea, in the State of Maranhão - CPSMV-CROT. A host range study evidenced biological differences among the virus isolates, especially in cowpea genotypes. The isolates were showed to be serologically and molecularly related by polymerase chain reaction – PCR, using degenerated primers which amplified two conserved regions in the coat protein and in the replicase genes. Cloning and sequence of CPSMV-PE made possible its comparison with other CPSMV isolates and other virus species from the genus Comovirus. Keywords: Vigna unguiculata, Comovirus, CPSMV, molecular differentiation.
650 $aComovirus
650 $aVigna Unguiculata
653 $aCPSMV
653 $aMolecular differentiation
700 1 $aNASCIMENTO, A. K. Q. do
700 1 $aANDRADE, E. C. de
700 1 $aLIMA, J. A. A.
773 $tTropical Plant Pathology, Brasília, DF$gv. 34, n. 4, p. 239-244, jul./ago. 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
