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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/01/2012 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, A. L. S.; COSTA, P. P.; MACHADO, M. A.; PAULA, C. M. P. de; SOUZA SOBRINHO, F. de. |
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Afiliação: |
ANA LUISA SOUSA AZEVEDO, CNPGL; PRICILA PALLA COSTA, CES/JF; MARCO ANTONIO MACHADO, CNPGL; CRISTINA MARIA PINTO DE PAULA, CES-JF; FAUSTO DE SOUZA SOBRINHO, CNPGL. |
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Título: |
High degree of genetic diversity among genotypes of the forage grass Brachiaria ruziziensis (Poaceae) detected with ISSR markers. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 4, p. 3530-3538, 2011. |
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DOI: |
http://dx.doi.org/10.4238/2011.November.17.5 |
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Idioma: |
Inglês |
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Conteúdo: |
The grasses of the genus Brachiaria account for 80% of the cultivated pastures in Brazil. Despite its importance for livestock production, little information is available for breeding purposes. Embrapa has a population of B. ruziziensis from different regions of Brazil, representing most of existing variability. This population was used to initiate an improvement program based on recurrent selection. In order to assist the genetic improvement program, we estimated the molecular variability among 93 genotypes of Embrapa's collection using ISSR (inter-simple sequence repeat) markers. DNA was extracted from the leaves. Twelve ISSR primers generated 89 polymorphic bands in the 93 genotypes. The number of bands identified by each primer ranged from two to 13, with a mean of 7.41. Cluster analysis revealed a clearly distinct group, containing most of the B. ruziziensis genotypes apart from the outgroup genotypes. Genetic similarity coefficients ranged from 0.0 to 0.95, with a mean of 0.50 and analysis of molecular variance indicated higher variation within (73.43%) than among species (26.57%). We conclude that there is a high genetic diversity among these B. ruziziensis genotypes, which could be explored by breeding programs. |
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Palavras-Chave: |
Genetic variability; Molecular markers. |
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Thesaurus Nal: |
forage. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51736/1/Artigo-meta-2011-GMR-Ana-Luisa-gmr1355.pdf
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Marc: |
LEADER 01976naa a2200217 a 4500
001 1911781
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2011.November.17.5$2DOI
100 1 $aAZEVEDO, A. L. S.
245 $aHigh degree of genetic diversity among genotypes of the forage grass Brachiaria ruziziensis (Poaceae) detected with ISSR markers.$h[electronic resource]
260 $c2011
520 $aThe grasses of the genus Brachiaria account for 80% of the cultivated pastures in Brazil. Despite its importance for livestock production, little information is available for breeding purposes. Embrapa has a population of B. ruziziensis from different regions of Brazil, representing most of existing variability. This population was used to initiate an improvement program based on recurrent selection. In order to assist the genetic improvement program, we estimated the molecular variability among 93 genotypes of Embrapa's collection using ISSR (inter-simple sequence repeat) markers. DNA was extracted from the leaves. Twelve ISSR primers generated 89 polymorphic bands in the 93 genotypes. The number of bands identified by each primer ranged from two to 13, with a mean of 7.41. Cluster analysis revealed a clearly distinct group, containing most of the B. ruziziensis genotypes apart from the outgroup genotypes. Genetic similarity coefficients ranged from 0.0 to 0.95, with a mean of 0.50 and analysis of molecular variance indicated higher variation within (73.43%) than among species (26.57%). We conclude that there is a high genetic diversity among these B. ruziziensis genotypes, which could be explored by breeding programs.
650 $aforage
653 $aGenetic variability
653 $aMolecular markers
700 1 $aCOSTA, P. P.
700 1 $aMACHADO, M. A.
700 1 $aPAULA, C. M. P. de
700 1 $aSOUZA SOBRINHO, F. de
773 $tGenetics and Molecular Research$gv. 10, n. 4, p. 3530-3538, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/05/2023 |
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Data da última atualização: |
05/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SILVA, N. M. L.; CAMPELO, A. A.; CAETANO, A. R.; IANELLA, P. |
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Afiliação: |
N. M. L. SILVA, Universidade de Brasília; A. A. CAMPELO, Universidade de Brasília; ALEXANDRE RODRIGUES CAETANO, Cenargen; PATRICIA IANELLA, Cenargen. |
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Título: |
Comparison of genomic DNA extraction methods for Colossoma macropomum fish fin clippings for single nucleotide polymorphism genotyping. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, GMR19105, 2023. |
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DOI: |
https://doi.org/10.4238/gmr19105 |
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Idioma: |
Inglês |
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Palavras-Chave: |
DNA extraction; Fin clippings; PCR amplification; Proteinase K. |
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Thesaurus NAL: |
Fish. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00717naa a2200217 a 4500
001 2153933
005 2023-12-05
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/gmr19105$2DOI
100 1 $aSILVA, N. M. L.
245 $aComparison of genomic DNA extraction methods for Colossoma macropomum fish fin clippings for single nucleotide polymorphism genotyping.$h[electronic resource]
260 $c2023
650 $aFish
653 $aDNA extraction
653 $aFin clippings
653 $aPCR amplification
653 $aProteinase K
700 1 $aCAMPELO, A. A.
700 1 $aCAETANO, A. R.
700 1 $aIANELLA, P.
773 $tGenetics and Molecular Research$gv. 22, n. 1, GMR19105, 2023.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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