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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
10/02/2017 |
Data da última atualização: |
29/03/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LOBO, F. P.; CINTRA, L. C.; VARELA, E. S.; ALVES, A. L.; SILVA, N. M. A. da; IANELLA, P.; PAIVA, S. R.; CAETANO, A. R. |
Afiliação: |
FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; PATRICIA IANELLA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN. |
Título: |
Genome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer). |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s. n.], 2016. |
Páginas: |
Não paginado. |
Idioma: |
Inglês |
Notas: |
PAG 2016. Pôster P0481. |
Conteúdo: |
The South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities. MenosThe South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource ... Mostrar Tudo |
Palavras-Chave: |
Bioinformática; Genômica; Sequenciamento de genoma. |
Thesaurus Nal: |
Bioinformatics; Genome assembly; Genomics; Sequence analysis. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140246/1/PAG-p0481.pdf
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Marc: |
LEADER 02464nam a2200301 a 4500 001 2063462 005 2023-03-29 008 2016 bl uuuu u01u1 u #d 100 1 $aLOBO, F. P. 245 $aGenome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer).$h[electronic resource] 260 $aIn: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s. n.]$c2016 300 $aNão paginado. 500 $aPAG 2016. Pôster P0481. 520 $aThe South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities. 650 $aBioinformatics 650 $aGenome assembly 650 $aGenomics 650 $aSequence analysis 653 $aBioinformática 653 $aGenômica 653 $aSequenciamento de genoma 700 1 $aCINTRA, L. C. 700 1 $aVARELA, E. S. 700 1 $aALVES, A. L. 700 1 $aSILVA, N. M. A. da 700 1 $aIANELLA, P. 700 1 $aPAIVA, S. R. 700 1 $aCAETANO, A. R.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
06/03/2017 |
Data da última atualização: |
06/03/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SPERANDIO, E. M.; FILIPPI, M. C. C. de; CORTES, M. V. de C. B.; VALE, H. M. M. do. |
Afiliação: |
EUGENIO MIRANDA SPERANDIO, doutorando UNB; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; HELSON MARIO MARTINS DO VALE, UNB. |
Título: |
Quantificação de B-1,3-glucanase e ácido salicílico em plantas de arroz induzidas com isolado avirulento de Magnaporthe oryzae. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. |
Páginas: |
p. 91. |
Série: |
(Embrapa Arroz e Feijão. Documentos, 311). |
Idioma: |
Português |
Conteúdo: |
O objetivo foi induzir resistência sistêmica por meio de isolado avirulentos de Magnaporthe oryzae e quantificar atividade de B-1,3-glucanase e teor de ácido salicílico em plantas de arroz. |
Thesagro: |
Arroz; Brusone; Doença de planta; Oryza sativa. |
Thesaurus NAL: |
Magnaporthe oryzae. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157135/1/p91.pdf
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Marc: |
LEADER 01051nam a2200229 a 4500 001 2066206 005 2017-03-06 008 2016 bl uuuu u00u1 u #d 100 1 $aSPERANDIO, E. M. 245 $aQuantificação de B-1,3-glucanase e ácido salicílico em plantas de arroz induzidas com isolado avirulento de Magnaporthe oryzae.$h[electronic resource] 260 $aIn: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão$c2016 300 $ap. 91. 490 $a(Embrapa Arroz e Feijão. Documentos, 311). 520 $aO objetivo foi induzir resistência sistêmica por meio de isolado avirulentos de Magnaporthe oryzae e quantificar atividade de B-1,3-glucanase e teor de ácido salicílico em plantas de arroz. 650 $aMagnaporthe oryzae 650 $aArroz 650 $aBrusone 650 $aDoença de planta 650 $aOryza sativa 700 1 $aFILIPPI, M. C. C. de 700 1 $aCORTES, M. V. de C. B. 700 1 $aVALE, H. M. M. do
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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