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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
12/08/2019 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOMINGUETEA, L. C. B.; LIMA, P. V. M.; NUNES, I. V.; SANDE, D.; LANA, U. G. de P.; MARRIEL, I. E.; TAKAHASHI, J. A. |
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Afiliação: |
Laura C. B. Dominguetea, Universidade Federal de Minas Gerais; Paulo V. M. Lima, Universidade Federal de Minas Gerais; Isabela V. Nunes, Universidade Federal de Minas Gerais; Denise Sande, Centro Universitário de Belo Horizonte; UBIRACI GOMES DE PAULA LANA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; Jacqueline A. Takahashi, Universidade Federal de Minas Gerais. |
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Título: |
Epigenetic-induced production of promising food supplements ingredients with antioxidant and anti-acetylcholinesterase activities |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Chemical Engineering Transactions, v. 74, p. 331-336, 2019. |
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DOI: |
10.3303/CET1975056 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungal metabolites with antioxidant and acetylcholinesterase inhibitory activities can be developed as food supplements helpful for treatment of neurodegenerative diseases, like Alzheimer's disease. Fungal biosynthesis depends on the expression of several enzymes that can change chromatin?s conformation, provoking activation or silencing of gene transcription. In this sense, the use of epigenetic modulators, able to alter chromatin?s conformation, has been a modern technique used to induce the expression of silenced genes in fungi. In the present study the metabolic profile and the antioxidant and anti-acetylcholinesterase activities of the extracts of Penicillium flavigenum (PF) and P. tanzanicum (PT) grown in the presence of epigenetic modulators were analyzed. The fungi were grown in liquid media, with or without (control) the epigenetic modulators azacytidine (AZA), hydralazine (HYDRA), procainamide (PRO), suberoyl hydroxamic acid (SAHA) and sodium butyrate (BUT) (500 uM). The modulators changed the expression of exogenous metabolites produced by the fungi with overexpression of some compounds in comparison to the controls, according to the HPLC chromatographic profiles. The treatment PF-SAHA presented the higher content of total phenolic compounds (27.86 mg GAE/g) (together with PF-HYDRA and PF-BUT) and the higher total antioxidant content (25.70 mg AAE/g). Besides, PF-SAHA and PF-PRO extracts presented higher antiacetylcholinesterase activity compared to others extracts. AZA was one of the most effective treatments for increasing expression of metabolites with antioxidant activity by P. tanzanicum in at least half of the assays. Treatments PT-AZA, PT-HYDRA and PT-BUT showed statistically similar anti-acetylcholinesterase activity, which were superior to the activity of the fungal control (15%). In overall, fermentative conditions using PFSAHA and PF-PRO showed to be the most favorable to produce new bioactive metabolites to be used as food supplements for palliative treatment or prevention of Alzheimer?s disease, while complete cure is not possible so far. MenosFungal metabolites with antioxidant and acetylcholinesterase inhibitory activities can be developed as food supplements helpful for treatment of neurodegenerative diseases, like Alzheimer's disease. Fungal biosynthesis depends on the expression of several enzymes that can change chromatin?s conformation, provoking activation or silencing of gene transcription. In this sense, the use of epigenetic modulators, able to alter chromatin?s conformation, has been a modern technique used to induce the expression of silenced genes in fungi. In the present study the metabolic profile and the antioxidant and anti-acetylcholinesterase activities of the extracts of Penicillium flavigenum (PF) and P. tanzanicum (PT) grown in the presence of epigenetic modulators were analyzed. The fungi were grown in liquid media, with or without (control) the epigenetic modulators azacytidine (AZA), hydralazine (HYDRA), procainamide (PRO), suberoyl hydroxamic acid (SAHA) and sodium butyrate (BUT) (500 uM). The modulators changed the expression of exogenous metabolites produced by the fungi with overexpression of some compounds in comparison to the controls, according to the HPLC chromatographic profiles. The treatment PF-SAHA presented the higher content of total phenolic compounds (27.86 mg GAE/g) (together with PF-HYDRA and PF-BUT) and the higher total antioxidant content (25.70 mg AAE/g). Besides, PF-SAHA and PF-PRO extracts presented higher antiacetylcholinesterase activity compared to others extract... Mostrar Tudo |
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Palavras-Chave: |
Doença neurodegenerativa. |
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Thesagro: |
Enzima; Fungo. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200582/1/Epigenetic-induced.pdf
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Marc: |
LEADER 02852naa a2200241 a 4500
001 2111259
005 2020-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3303/CET1975056$2DOI
100 1 $aDOMINGUETEA, L. C. B.
245 $aEpigenetic-induced production of promising food supplements ingredients with antioxidant and anti-acetylcholinesterase activities$h[electronic resource]
260 $c2019
520 $aFungal metabolites with antioxidant and acetylcholinesterase inhibitory activities can be developed as food supplements helpful for treatment of neurodegenerative diseases, like Alzheimer's disease. Fungal biosynthesis depends on the expression of several enzymes that can change chromatin?s conformation, provoking activation or silencing of gene transcription. In this sense, the use of epigenetic modulators, able to alter chromatin?s conformation, has been a modern technique used to induce the expression of silenced genes in fungi. In the present study the metabolic profile and the antioxidant and anti-acetylcholinesterase activities of the extracts of Penicillium flavigenum (PF) and P. tanzanicum (PT) grown in the presence of epigenetic modulators were analyzed. The fungi were grown in liquid media, with or without (control) the epigenetic modulators azacytidine (AZA), hydralazine (HYDRA), procainamide (PRO), suberoyl hydroxamic acid (SAHA) and sodium butyrate (BUT) (500 uM). The modulators changed the expression of exogenous metabolites produced by the fungi with overexpression of some compounds in comparison to the controls, according to the HPLC chromatographic profiles. The treatment PF-SAHA presented the higher content of total phenolic compounds (27.86 mg GAE/g) (together with PF-HYDRA and PF-BUT) and the higher total antioxidant content (25.70 mg AAE/g). Besides, PF-SAHA and PF-PRO extracts presented higher antiacetylcholinesterase activity compared to others extracts. AZA was one of the most effective treatments for increasing expression of metabolites with antioxidant activity by P. tanzanicum in at least half of the assays. Treatments PT-AZA, PT-HYDRA and PT-BUT showed statistically similar anti-acetylcholinesterase activity, which were superior to the activity of the fungal control (15%). In overall, fermentative conditions using PFSAHA and PF-PRO showed to be the most favorable to produce new bioactive metabolites to be used as food supplements for palliative treatment or prevention of Alzheimer?s disease, while complete cure is not possible so far.
650 $aEnzima
650 $aFungo
653 $aDoença neurodegenerativa
700 1 $aLIMA, P. V. M.
700 1 $aNUNES, I. V.
700 1 $aSANDE, D.
700 1 $aLANA, U. G. de P.
700 1 $aMARRIEL, I. E.
700 1 $aTAKAHASHI, J. A.
773 $tChemical Engineering Transactions$gv. 74, p. 331-336, 2019.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
15/02/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, D. C. F.; BRUNELI, F. A. T.; COSTA, C. N.; PEIXOTO, M. G. C. D.; SANTOS, G. G. dos; FILHO, A. E. V.; FARO, L. E.; BONAFÉ, C. M. |
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Afiliação: |
DIANA CARLA F. OLIVEIRA; FRANK ANGELO TOMITA BRUNELI, CNPGL; CLAUDIO NAPOLIS COSTA, CNPGL; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; GLAUCYANA GOUVEA DOS SANTOS, CNPGL; ANIBAL E. VERCESI FILHO; LENIRA EL FARO; CRISTINA . BONAFÉ. |
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Título: |
Factors study associated with longevity of dairy zebu cows |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: INTERNATIONAL MEETING OF ADVANCES IN SCIENCE. v. 1. p. 1. Anais... Universidade Estadual Paulista. Jaboticabal, SP. 2016 |
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Páginas: |
1 |
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Idioma: |
Português |
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Palavras-Chave: |
Breeding zebu; Disposal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 00693naa a2200229 a 4500
001 2064175
005 2017-02-15
008 2016 bl --- 0-- u #d
100 1 $aOLIVEIRA, D. C. F.
245 $aFactors study associated with longevity of dairy zebu cows
260 $c2016
300 $a1
653 $aBreeding zebu
653 $aDisposal
700 1 $aBRUNELI, F. A. T.
700 1 $aCOSTA, C. N.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aSANTOS, G. G. dos
700 1 $aFILHO, A. E. V.
700 1 $aFARO, L. E.
700 1 $aBONAFÉ, C. M.
773 $tIn: INTERNATIONAL MEETING OF ADVANCES IN SCIENCE.$gv. 1. p. 1. Anais... Universidade Estadual Paulista. Jaboticabal, SP. 2016
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