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| Registros recuperados : 67 | |
| 2. |  | SCHEUERMANN, K. K.; BROMMONSCHENKEL, S. H. Análise da expressão diferencial de genes envolvidos na interação tomateiro-alternaria solani por PCR em tempo real. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S74, ago. 2006. Suplemento. Resumo 114. Trabalho apresentado no 38. Congresso Brasileiro de Fitopatologia, 2005, Brasília, DF.| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | NOVAES, E.; SOARES, B. L.; BROMMONSCHENKEL, S. H.; GRATTAPAGLIA, D. Localização de microssatélites em clones BAC de Eucalyptus. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 474.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | ROCHA, C. M.; SCHEUERMANN, K. K.; BROMMONSCHENKEL, S. H. Caracterização de dois fatores de transcrição WRKY expressos na interação tomateiro Alternaria solani. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S86, ago. 2006. Suplemento. Resumo 186. Trabalho apresentado no 38. Congresso Brasileiro de Fitopatologia, 2005, Brasília, DF.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | FIGUEIREDO, G.; ALFENAS, A. C.; BROMMONSCHENKEL, S. H.; FARIA, J. C. Isoenzimas como marcadores geneticos em Colletotrichum lindemuthianum. Fitopatologia Brasileira, v. 15, n. 2, p. 151, jul. 1990. Suplemento, ref. 189. Edição de Resumos do XXIII Congresso Brasileiro de Fitopatologia, Goiânia, jul. 1990.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 15. | | VAZ, A. R. C.; BRONDANI, R. P. V.; BROMMONSCHENKEL, S. H.; GRATTAPAGLIA, D. Ancoragem do mapa genético no mapa físico utilizando marcadores microssatélites a partir de uma biblioteca de BACs de Eucalyptus grandis. In: CONGRESSO BRASILEIRO DE GENÉTICA, 52., 2006, Foz do Iguaçu, PR. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. p. 1167.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | SCHEUERMANN, K. K.; FALLEIRO, B. A. S.; REIS, A.; BROMMONSCHENKEL, S. H.; MIZUBITI, E. S. G. Seleção de marcadores RAPD para estudo de diversidade genética de Alternaria solani. Fitopatologia Brasileira, Brasília, v. 29, p. S49-50, ago. 2004. Suplemento. Resumo. Trabalho apresentado no 37º Congresso Brasileiro de Fitopatologia, 2004.| Biblioteca(s): Embrapa Hortaliças. |
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| 19. | | LAU, D.; OLIVEIRA, J. C. F. de; LAU, Y. E.; BROMMONSCHENKEL, S. H. Hipersensibilidade e necrose sistêmica em Nicotiana benthamiana transformada com o gene de resistência Sw-5 de tomateiro. Fitopatologia Brasileira, Brasília, DF, v. 31, n. 3, p. 247-253, maio/jun. 2006| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 67 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. |
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Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS. |
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Título: |
Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Mycologia, New York, v. 106, n. 4, p. 629-641, 2014. |
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DOI: |
10.3852/13?262 |
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Idioma: |
Inglês |
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Conteúdo: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon MenosTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the char... Mostrar Tudo |
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Thesagro: |
Antracnose. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aBRAGA, R. M.
245 $aTransposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.$h[electronic resource]
260 $c2014
520 $aTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon
650 $aAntracnose
700 1 $aSANTANA, M. F.
700 1 $aCOSTA, R. V. da
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aARAÚJO, E. F. de
700 1 $aQUEIROZ, M. V. de
773 $tMycologia, New York$gv. 106, n. 4, p. 629-641, 2014.
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