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| Registros recuperados : 10 | |
| 1. |  | TREML, D.; VENTURELLI, G. L.; BROD, F. C. A.; FARIA, J. C.; ARISI, A. C. M. Development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris). Journal of Agricultural and Food Chemistry, Easton, v. 62, n. 49, p. 11994-12000, Dec. 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 2. | | BROD, F. C. A.; DINON, A. Z.; KOLLING, D. J.; FARIA, J. C.; ARISI, A. C. M. Development of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1. Journal of Agricultural and Food Chemistry, Easton, v. 61, n. 20, p. 4921-4926, May 2013.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 4. | | BROD, F. C. A.; PELISSER, M. R.; BERTOLDO, J. B.; VERNAL, J.; BLOCH JUNIOR, C.; TERENZI, H.; ARISI, A. C. M.; ARISI, A. C. M. Heterologous Expression and Purification of a Heat-Tolerant Staphylococcus xylosus Lipase. Molecular Biotechnology, v.44, n.2, p. 110-119, 2010| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | FONGARO, G.; RIBEIRO, J. B.; BERTOL, T. M.; FIRENTINI, A. M.; SAWITKI, M. C.; PEIXOTO, J. de O.; BROD, F. C. A.; ARISI, A. C. M. Isolamento de um fragmento do gene de lipase de staphylococcus xylosus U5 e AD1. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA/UNC, 4., 2010, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves, 2010. 1 CD-ROM. 4 JINC. Projeto/Plano de Ação: 03.08.06.009-02.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | SAWITZKI, M. C.; FIORENTINI, A. M.; BROD, F. C. A.; TAGLIARI, C.; BERTOL, T. M.; ARISI, A. C. M.; SANT'ANNA, E. S. Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages. Brazilian Journal of Microbiology, v.38, n3. p. 547-552, 2007. Projeto n. 16.00.30001-16.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | FIORENTINI, A. M.; SAWITZKI, M. C.; BERTOL, T. M.; BROD, F. C. A.; PELISSER, M. R.; ARISI, A. C. M.; SANT'ANNA, E. S. Phenotypic and molecular characterization of staphylococcus xylosus: technological potential for use in fermented sausage Brazilian Archives of Biology and Technology, Curitiba, v. 52, n.3, p. 737-746. May/June 2009. Disponível em: . Acesso em: 09 fev. 2010 Projeto/Plano da Ação: 01.06.60.106-07| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; SILVEIRA, J. C. M.; KOLLING, D. J.; TREML, D.; FARIA, J. C.; ARISI, A. C. M. Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; OLIVEIRA, E. M. M.; FARIA, J. C.; ARISI, A. C. M. Primers and probes development for specific PCR detection of genetically modified common bean (Phaseolus vulgaris) Embrapa 5.1. Journal of Agricultural and Food Chemistry, Easton, v. 60, n. 18, p. 4672-4677, May 2012.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Arroz e Feijão. |
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| 10. | | VENTURELLI, G. L.; BROD, F. C. A.; ROSSI, G. B.; ZIMMERMANN, N. F.; OLIVEIRA, J. P.; FARIA, J. C.; ARISI, A. C. M. A specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene. Molecular Biotechnology, Totowa, v. 56, n. 11, p. 1060-1068, Nov. 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 10 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/09/2016 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TREML, D.; VENTURELLI, G. L.; BROD, F. C. A.; FARIA, J. C.; ARISI, A. C. M. |
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Afiliação: |
DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA; GUSTAVO L. VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; FABIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Easton, v. 62, n. 49, p. 11994-12000, Dec. 2014. |
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DOI: |
10.1021/jf503928m |
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Idioma: |
Inglês |
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Conteúdo: |
A genetically modified (GM) common bean event, namely Embrapa 5.1, resistant to the bean golden mosaic virus (BGMV), was approved for commercialization in Brazil. Brazilian regulation for genetically modified organism (GMO) labeling requires that any food containing more than 1% GMO be labeled. The event-specific polymerase chain reaction (PCR) method has been the primary trend for GMO identification and quantitation because of its high specificity based on the flanking sequence. This work reports the development of an event-specific assay, named FGM, for Embrapa 5.1 detection and quantitation by use of SYBR Green or hydrolysis probe. The FGM assay specificity was tested for Embrapa 2.3 event (a noncommercial GM common bean also resistant to BGMV), 46 non-GM common bean varieties, and other crop species including maize, GM maize, soybean, and GM soybean. The FGM assay showed high specificity to detect the Embrapa 5.1 event. Standard curves for the FGM assay presented a mean efficiency of 95% and a limit of detection (LOD) of 100 genome copies in the presence of background DNA. The primers and probe developed are suitable for the detection and quantitation of Embrapa 5.1. |
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Palavras-Chave: |
GMO; QPCR; SYBR green; TaqMan. |
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Thesagro: |
Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Phaseolus vulgaris. |
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Thesaurus NAL: |
Beans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2052475
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008 2014 bl uuuu u00u1 u #d
024 7 $a10.1021/jf503928m$2DOI
100 1 $aTREML, D.
245 $aDevelopment of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris).$h[electronic resource]
260 $c2014
520 $aA genetically modified (GM) common bean event, namely Embrapa 5.1, resistant to the bean golden mosaic virus (BGMV), was approved for commercialization in Brazil. Brazilian regulation for genetically modified organism (GMO) labeling requires that any food containing more than 1% GMO be labeled. The event-specific polymerase chain reaction (PCR) method has been the primary trend for GMO identification and quantitation because of its high specificity based on the flanking sequence. This work reports the development of an event-specific assay, named FGM, for Embrapa 5.1 detection and quantitation by use of SYBR Green or hydrolysis probe. The FGM assay specificity was tested for Embrapa 2.3 event (a noncommercial GM common bean also resistant to BGMV), 46 non-GM common bean varieties, and other crop species including maize, GM maize, soybean, and GM soybean. The FGM assay showed high specificity to detect the Embrapa 5.1 event. Standard curves for the FGM assay presented a mean efficiency of 95% and a limit of detection (LOD) of 100 genome copies in the presence of background DNA. The primers and probe developed are suitable for the detection and quantitation of Embrapa 5.1.
650 $aBeans
650 $aBiotecnologia
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPhaseolus vulgaris
653 $aGMO
653 $aQPCR
653 $aSYBR green
653 $aTaqMan
700 1 $aVENTURELLI, G. L.
700 1 $aBROD, F. C. A.
700 1 $aFARIA, J. C.
700 1 $aARISI, A. C. M.
773 $tJournal of Agricultural and Food Chemistry, Easton$gv. 62, n. 49, p. 11994-12000, Dec. 2014.
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