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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
28/11/1994 |
Data da última atualização: |
14/05/1997 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MAGALHAES, B. P.; HUMBER, R. A.; SHIELDS E. J.; ROBERTS, D. W. |
Afiliação: |
EMBRAPA-CENARGEN. |
Título: |
Effects of environment and nutrition on conidium germination and appressorium formation by Zoophthora radicans (Zygomycetes: Entomophthorales): a pathogen of the potato leafhopper (Homoptera: Cicadellidae). |
Ano de publicação: |
1991 |
Fonte/Imprenta: |
Environmental Entomology, Lanham, v.20, n.5, p.1460-1468, 1991. |
Idioma: |
Inglês |
Conteúdo: |
Conidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appressoria on cuticles of dead Empoasca fabae (Harris) nymphs at 25°C in the dark. MenosConidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appress... Mostrar Tudo |
Palavras-Chave: |
Conidium; Grasshopper. |
Thesagro: |
Conídio; Empoasca Fabae; Fungo; Gafanhoto; Germinação; Zoophthora Radicans. |
Thesaurus Nal: |
Empoasca; germination; Insect pathology; Insects. |
Categoria do assunto: |
-- O Insetos e Entomologia |
Marc: |
LEADER 02497naa a2200301 a 4500 001 1170965 005 1997-05-14 008 1991 bl uuuu u00u1 u #d 100 1 $aMAGALHAES, B. P. 245 $aEffects of environment and nutrition on conidium germination and appressorium formation by Zoophthora radicans (Zygomycetes$bEntomophthorales): a pathogen of the potato leafhopper (Homoptera: Cicadellidae). 260 $c1991 520 $aConidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appressoria on cuticles of dead Empoasca fabae (Harris) nymphs at 25°C in the dark. 650 $aEmpoasca 650 $agermination 650 $aInsect pathology 650 $aInsects 650 $aConídio 650 $aEmpoasca Fabae 650 $aFungo 650 $aGafanhoto 650 $aGerminação 650 $aZoophthora Radicans 653 $aConidium 653 $aGrasshopper 700 1 $aHUMBER, R. A. 700 1 $aSHIELDS E. J. 700 1 $aROBERTS, D. W. 773 $tEnvironmental Entomology, Lanham$gv.20, n.5, p.1460-1468, 1991.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
12/03/2021 |
Data da última atualização: |
13/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BRITO, N. M. de; DUARTE, H. da S. S.; AUER, C. G.; WENDLING, I.; ALFENAS, R. F.; SANTOS, A. F. dos. |
Afiliação: |
NÍCOLAS MANARIM DE BRITO, UFPR; HENRIQUE DA SILVA SILVEIRA DUARTE, UFPR; CELSO GARCIA AUER, CNPF; IVAR WENDLING, CNPF; RAFAEL FERREIRA ALFENAS, UFV; ÁLVARO FIGUEREDO DOS SANTOS, Pesquisador aposentado da Embrapa Florestas. |
Título: |
Aggressiveness and screening for host responses in yerba mate (Ilex paraguariensis) clones to a wilt disease caused by Ceratocystis fimbriata. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Forest Pathology, v. 51, n. 3, e12682, June 2021. 8 p. |
Idioma: |
Inglês |
Conteúdo: |
Ceratocystis wilt of yerba mate (Ilex paraguariensis) caused by the fungus Ceratocystis fimbriata was observed in 2018 in commercial plantations located in São Mateus do Sul, PR and Arvorezinha, RS, Brazil, where mortality of the plants was up to 5%. Due to the need of control measures for this disease and to the lack of knowledge on the impact of this pathogen this new host, this study aimed to evaluate the aggressiveness of C. fimbriata isolates collected from yerba mate plantations and screen for host responses in yerba mate clones to Ceratocystis wilt. Two experiments were conducted in a completely randomized design, with 11 treatments (isolates) to assess aggressiveness of the isolates. Two experiments were conducted to assess resistance of yerba mate to C. fimbriata in a completely randomized design in a 6 × 2 factorial scheme (6 clones × 2 isolates) in two different environmental conditions (plant growth chamber and greenhouse). The isolates of C. fimbriata showed different levels of aggressiveness to yerba mate, and all evaluated clones were susceptible to Ceratocystis wilt. The plant growth chamber condition led to a faster death of the plants compared with greenhouse. This information will be useful to understand the pathosystem and seek strategies for controlling this disease in yerba mate. |
Palavras-Chave: |
Ceratocystis wilt; Murcha vascular; Resistence. |
Thesagro: |
Ceratocystis Fimbriata; Fungo; Ilex Paraguariensis; Resistência. |
Thesaurus NAL: |
Vascular wilt. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02177naa a2200277 a 4500 001 2130639 005 2021-07-13 008 2021 bl uuuu u00u1 u #d 100 1 $aBRITO, N. M. de 245 $aAggressiveness and screening for host responses in yerba mate (Ilex paraguariensis) clones to a wilt disease caused by Ceratocystis fimbriata.$h[electronic resource] 260 $c2021 520 $aCeratocystis wilt of yerba mate (Ilex paraguariensis) caused by the fungus Ceratocystis fimbriata was observed in 2018 in commercial plantations located in São Mateus do Sul, PR and Arvorezinha, RS, Brazil, where mortality of the plants was up to 5%. Due to the need of control measures for this disease and to the lack of knowledge on the impact of this pathogen this new host, this study aimed to evaluate the aggressiveness of C. fimbriata isolates collected from yerba mate plantations and screen for host responses in yerba mate clones to Ceratocystis wilt. Two experiments were conducted in a completely randomized design, with 11 treatments (isolates) to assess aggressiveness of the isolates. Two experiments were conducted to assess resistance of yerba mate to C. fimbriata in a completely randomized design in a 6 × 2 factorial scheme (6 clones × 2 isolates) in two different environmental conditions (plant growth chamber and greenhouse). The isolates of C. fimbriata showed different levels of aggressiveness to yerba mate, and all evaluated clones were susceptible to Ceratocystis wilt. The plant growth chamber condition led to a faster death of the plants compared with greenhouse. This information will be useful to understand the pathosystem and seek strategies for controlling this disease in yerba mate. 650 $aVascular wilt 650 $aCeratocystis Fimbriata 650 $aFungo 650 $aIlex Paraguariensis 650 $aResistência 653 $aCeratocystis wilt 653 $aMurcha vascular 653 $aResistence 700 1 $aDUARTE, H. da S. S. 700 1 $aAUER, C. G. 700 1 $aWENDLING, I. 700 1 $aALFENAS, R. F. 700 1 $aSANTOS, A. F. dos 773 $tForest Pathology$gv. 51, n. 3, e12682, June 2021. 8 p.
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