|
|
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
10/12/2019 |
Data da última atualização: |
10/12/2019 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
MACHADO, J. C.; PEREIRA, J. F.; AZEVEDO, A. L. S.; MITTELMANN, A.; PEREIRA, A. V.; SOUZA SOBRINHO, F. de; BENITES, F. R. G.; LEDO, F. J. da S. |
Afiliação: |
JUAREZ CAMPOLINA MACHADO, CNPGL; JORGE FERNANDO PEREIRA, CNPGL; ANA LUISA SOUSA AZEVEDO, CNPGL; ANDREA MITTELMANN, CNPGL; ANTONIO VANDER PEREIRA, CNPGL; FAUSTO DE SOUZA SOBRINHO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; FRANCISCO JOSE DA SILVA LEDO, CNPGL. |
Título: |
Melhoramento genético de forrageiras e o uso de ferramentas genômicas. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: AZEVEDO, A. L. S.; PEREIRA, J. F.; MACHADO, J. C. (Ed.). Melhoramento de forrageiras na era genômica. Brasília, DF: Embrapa, 2019. |
Páginas: |
p. 11-42. |
Idioma: |
Português |
Conteúdo: |
Novas cultivares de diferentes espécies de forrageiras ajudam a aumentar a diversidade e a produtividade das pastagens brasileiras e a manter a produção animal com preços competitivos nos mercados nacional e internacional. |
Palavras-Chave: |
Forrageiras temperadas; Forrageiras tropicais; Gramíneas principais; Leguminosas forrageiras. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 01073naa a2200265 a 4500 001 2116533 005 2019-12-10 008 2019 bl uuuu u00u1 u #d 100 1 $aMACHADO, J. C. 245 $aMelhoramento genético de forrageiras e o uso de ferramentas genômicas. 260 $c2019 300 $ap. 11-42. 520 $aNovas cultivares de diferentes espécies de forrageiras ajudam a aumentar a diversidade e a produtividade das pastagens brasileiras e a manter a produção animal com preços competitivos nos mercados nacional e internacional. 653 $aForrageiras temperadas 653 $aForrageiras tropicais 653 $aGramíneas principais 653 $aLeguminosas forrageiras 700 1 $aPEREIRA, J. F. 700 1 $aAZEVEDO, A. L. S. 700 1 $aMITTELMANN, A. 700 1 $aPEREIRA, A. V. 700 1 $aSOUZA SOBRINHO, F. de 700 1 $aBENITES, F. R. G. 700 1 $aLEDO, F. J. da S. 773 $tIn: AZEVEDO, A. L. S.; PEREIRA, J. F.; MACHADO, J. C. (Ed.). Melhoramento de forrageiras na era genômica. Brasília, DF: Embrapa, 2019.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
14/12/2023 |
Data da última atualização: |
11/03/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SILVA, J. R. da; SILVA, J. F. M.; PEREIRA, M. F.; TORRES, A. R.; GONÇALVES, M. S.; PRATA, M. C. de A.; BRITO, M. A. V. P. e; COSTA, G. M. da; RIBEIRO, J. B. |
Afiliação: |
JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL. |
Título: |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. |
DOI: |
https://doi.org/10.1007/s42770-023-01181-1 |
Idioma: |
Inglês |
Conteúdo: |
Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. MenosStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic tar... Mostrar Tudo |
Palavras-Chave: |
Infecção intramamária; Modelo de invertebrados; Virulência bacteriana. |
Thesagro: |
Bovino; Doença Animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/262967/1/Is-Galleria-mellonella-model-a-good-alternative-to-study-virulence.pdf
|
Marc: |
LEADER 02652naa a2200289 a 4500 001 2159710 005 2024-03-11 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-023-01181-1$2DOI 100 1 $aSILVA, J. R. da 245 $aIs Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?$h[electronic resource] 260 $c2024 520 $aStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. 650 $aBovino 650 $aDoença Animal 653 $aInfecção intramamária 653 $aModelo de invertebrados 653 $aVirulência bacteriana 700 1 $aSILVA, J. F. M. 700 1 $aPEREIRA, M. F. 700 1 $aTORRES, A. R. 700 1 $aGONÇALVES, M. S. 700 1 $aPRATA, M. C. de A. 700 1 $aBRITO, M. A. V. P. e 700 1 $aCOSTA, G. M. da 700 1 $aRIBEIRO, J. B. 773 $tBrazilian Journal of Microbiology$gv. 55, n. 1, p. 889-900, 2024.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|