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| Registros recuperados : 233 | |
| 161. |  | FURTADO, M. A. M.; COSTA, F. F.; PINTO, I. S. B.; PEREIRA, D. A.; GUIMARAES, M. F. M.; BRITO, M. A. V. P. e; PAIVA, I. M. de. Utilização de eletroforese "Lab-on-a-chip" para detecção de adulteração de leite com soro de queijo. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 4., 2010, Florianópolis. Anais... Florianópolis: CBQL, 2010. 3 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 162. | | SANTOS, E. M. P. dos; BRITO, M. A. V. P. e; LANGE, C. C.; BRITO, J. R. F.; CERQUEIRA, M. M. O. P. Streptococcus e gêneros relacionados como agentes etiológicos de mastite bovina. Acta Scientiae Veterinariae, Porto Alegre, v. 35, n. 1, p. 17-27, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 163. | | SANTOS, D. C. dos; LANGE, C. C.; AVELLAR-COSTA, P.; SANTOS, K. R. N. dos; BRITO, M. A. V. P. e; GIAMBIAGI-deMARVAL, M. Staphylococcus chromogenes: a coagulase-negative Staphylococcus that can clot plasma. Journal of Clinical Microbiology, v. 54, n. 5, p. 1372-1375, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 164. | | OLIVEIRA-PAIVA, C. A.; OLIVEIRA, M. C. de; MARRIEL, I. E.; GOMES, E. A.; CASTRO, C. S. P. DE; BRITO, M. A. V. P. e; COELHO, E. A.; MEIRELLES, W. C. L. Banco de competências: ferramenta de apoio à decisão de capacitação das coleções de microrganismos da Embrapa. Sete Lagoas: Embrapa Milho e Sorgo, 2015. 68 p. (Embrapa Milho e Sorgo. Documentos, 194).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 165. | | PAIVA, C. A. O.; OLIVEIRA, M. C. de; MARRIEL, I. E.; GOMES, E. A.; CASTRO, C. S. P. DE; BRITO, M. A. V. P. e; COELHO, E. A.; MEIRELLES, W. C. L. Banco de competências: ferramenta de apoio à decisão de capacitação das coleções de microrganismos da Embrapa. Sete Lagoas: Embrapa Milho e Sorgo, 2015 68 p. (Embrapa Milho e Sorgo. Documentos, 194).| Biblioteca(s): Embrapa Gado de Leite. |
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| 166. | | MOREIRA, M. S. de P.; RIBEIRO, A. C. de C. L.; CARVALHO, A. da C.; SANTOS, C. A. dos; ARCURI, E. F.; DINIZ, F. H.; SOUZA, G. N. de; BRITO, M. A. V. P. e; ZOCCAL, R. Kit Embrapa de ordenha manual. Juiz de Fora: Embrapa Gado de Leite, 2007. 20 p.| Biblioteca(s): Embrapa Agrobiologia. |
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| 167. | | SILVA, J. R. da; SILVA, J. F. M.; PEREIRA, M. F.; TORRES, A. R.; GONÇALVES, M. S.; PRATA, M. C. de A.; BRITO, M. A. V. P. e; COSTA, G. M. da; RIBEIRO, J. B. Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024.| Biblioteca(s): Embrapa Gado de Leite. |
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| 168. | | SILVA, T. N. B. da; OLIVEIRA, T. C. de A.; BRITO, M. A. V. P. e; SILVA, M. A. S.; MENDONCA, L. C.; LANGE, C. C.; SOUZA, G. N. de. Isolamento e identificação de patógenos da mastite nos Campos Experimentais da Embrapa Gado de Leite. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 17., 2016, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2016. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 186).| Biblioteca(s): Embrapa Gado de Leite. |
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| 169. | | LAPORT, M. S.; MARINHO, P. R.; SANTOS, O. C. DA S.; ALMEIDA, P. DE; ROMANOS, M. T. V.; MURICY, G.; BRITO, M. A. V. P. e; GIAMBIAGI-DE-MARVAL, M. Antimicrobial activity of marine sponges against coagulase-negative staphylococci isolated from bovine mastitis. Veterinary Microbiology, v. 155, p. 362-368, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 170. | | AIZAWA, J.; SOUZA FILHO, A. F.; BRITO, M. A. V. P. e; LANGE, C. C.; GUIMARAES, A. S.; SANTOS, R. P.; SOUZA, F. N.; SILVEIRA, A. C. O.; VASCONCELOS, C. G. C.; CORTEZ, A.; HEINEMANN, M. B. Absence of methicillin resistance in staphylococcus aureus isolated from bovine mastitis in southeast Brazil In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14., 2016, Rosário. Resúmenes. Buenos Aires: Asociación Argentina de Microbiología, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 171. | | NASCIMENTO, G. C.; PINHEIRO, M. L. M.; COSTA, L. C.; PINHEIRO, R. O.; MARQUES, S. L. S.; PACIULLI, S. O.; BRITO, M. A. V. P. e; PAULA, A. C. F. F. De; TEIXEIRA, R. B. Natural solutions for pre and post-dipping In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, v. 53. p. 1. Anais... Gramado, RS, 2016 1| Biblioteca(s): Embrapa Gado de Leite. |
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| 172. | | COSTA, F. F.; BRITO, M. A. V. P. e; FURTADO, M. A. M.; MARTINS, M. F.; OLIVEIRA, M. A. L. de; BARRA, P. M. de C.; GARRIDO, L. A.; SANTOS, A. S. de O. Microfluidic chip electrophoresis investigation of major milk proteins: study of buffer effects and quantitative approaching Analytical Methods, v. 6, p. 1666-1673, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| 173. | | LADEIRA, C. V.; SOUZA, F. N.; FREITAS, D. S.; LADEIRA, L. G.; RODRIGUES, D. S.; LEITE, M. O.; FONSECA, L. M.; PENNA, C. M.; BRITO, M. A. V. P. e; CERQUEIRA, M. P. Dynamic of intramammary infections in 3/4 holstein x zebu dairy cows from a herd of Minas Gerais state, Brazil. Journal of Animal Science, v. 92, p. 436, 2014. Supplement. Edição dos abstracts do Joint Annual Meeting, 2014, Kansas City.| Biblioteca(s): Embrapa Gado de Leite. |
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| 174. | | BARRETO, D. K.; LANGE, C. C.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; QUEIROZ, L. da S.; SILVA, T. N. B. da; MARTINI, C. L. Pesquisa de genes relacionados à produção de cápsula e biofilme em Staphylococcus aureus isolados de leite bovino. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 175. | | COSTA, F. C. B. da; OLIVEIRA, T. C. de A.; SILVA, M. A. S.; VICENTINI, N. M.; PIRES, M. de F. A.; DINIZ, F. H.; REIS, E. S. dos; BRITO, M. A. V. P. e; LANGE, C. C. Pesquisa de Listeria monocytogenes e Salmonella spp. e contagem de Staphylococcus spp. coagulase positivos e coliformes em queijo artesanal produzido no município de Alagoa, Minas Gerais. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 19., 2017, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2017. (Embrapa Gado de Leite. Documentos, 200.). 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 176. | | VARGAS, R. T.; SOUZA, F. N.; BRITO, M. A. V. P. e; BRITO, J. R. F.; LEITE, M. O.; FONSECA, L. M.; SAMPAIO, I. B. M.; CERQUEIRA, M. M. O. P. Partial budget analysis of prepartum antimicrobial therapy and Escherichia coli J5 vaccination of dairy heifers and their effect on milk production and milk quality parameters. Pesquisa Veterinária Brasileira, v. 36, n. 2, p. 77-82, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 177. | | VARGAS, R. T.; SOUZA, F. N.; BRITO, M. A. V. P. e; BRITO, J. R. F.; LEITE, M. O.; FONSECA, L. M.; SAMPAIO, I. B. M.; CERQUEIRA, M. M. O. P. Partial budget analysis of prepartum antimicrobial therpy and Escherichia coli J5 vaccination of dairy heifers and their effect on milk production and milk quality parameters. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 77-82, fev. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 178. | | RIBEIRO, J. B.; ALVARENGA, P. dos A.; RIBEIRO, G. R.; LEITE, S. J. de O.; MENDONCA, L. C.; LANGE, C. C.; GUIMARAES, A. S.; BRITO, M. A. V. P. e. Ocorrência de genes de fatores de virulência em staphylococcus aureus isolados de amostras de leite bovino. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 6., 2015, Curitiba. Anais... Curitiba: CBQL, 2015. p. 127-128.| Biblioteca(s): Embrapa Gado de Leite. |
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| 179. | | FONSECA, I.; PEREIRA, W. P.; CARNEIRO, J. da C.; BRITO, M. A. V. P. e; FERREIRA, M. B. D.; BRANDAO, H. de M.; GUIMARÃES, S. E. F.; MARTINS, M. F. Perfil de expressão do gene TGF-B1 em vacas gir infectadas com Streptococcus agalactiae. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 180. | | SANTOS, F. F.; MENDONCA, L. C.; GUIMARAES, A. S.; RIBEIRO, J. B.; LANGE, C. C.; SILVA, M. A. S.; OLIVEIRA, B. L.; MENDONÇA, R. S. DE; MACHADO, M. A.; BRITO, M. A. V. P. e. Perfil de resistência a antimicrobianos de Staphylococcus spp. resistentes a oxacilina isolados de leite bovino. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 5., 2013, Botucatu. Anais. Curitiba: Conselho Brasileiro de Qualidade do Leite, 2013. p. 98-99.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 233 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, F. F. dos; MENDONCA, L. C.; REIS, D. R. de L.; GUIMARAES, A. S.; LANGE, C. C.; RIBEIRO, J. B.; MACHADO, M. A.; BRITO, M. A. V. P. e. |
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Afiliação: |
Fernanda Fernandes dos Santos, UFJF; LETICIA CALDAS MENDONCA, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; CARLA CHRISTINE LANGE, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL. |
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Título: |
Presence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 2, p. 1374-1382, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures. MenosAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, ery... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial resistance; MecA; Methicillin-resistant staphylococci; SCCmec. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03425naa a2200253 a 4500
001 2061479
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. F. dos
245 $aPresence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil.$h[electronic resource]
260 $c2016
520 $aAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures.
653 $aAntimicrobial resistance
653 $aMecA
653 $aMethicillin-resistant staphylococci
653 $aSCCmec
700 1 $aMENDONCA, L. C.
700 1 $aREIS, D. R. de L.
700 1 $aGUIMARAES, A. S.
700 1 $aLANGE, C. C.
700 1 $aRIBEIRO, J. B.
700 1 $aMACHADO, M. A.
700 1 $aBRITO, M. A. V. P. e
773 $tJournal of Dairy Science$gv. 99, n. 2, p. 1374-1382, 2016.
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