Data/hora: 08/06/2024 05:46:15

Biblioteca(s): | Embrapa Café.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 18/02/2011
Data da última atualização: | 10/10/2012
Autoria: | BRITO, G. G. de; CAIXETA, E. T.; GALLINA, A. P.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; DIOLA, V.; LOUREIRO, M. E.
Afiliação: | GIOVANI GREIGH DE BRITO, CNPA; EVELINE TEIXEIRA CAIXETA, SAPC; ANA PAULA GALLINA, Universidade Federal de Viçosa; EUNIZE MACIEL ZAMBOLIM, Universidade Federal de Viçosa; LAÉRCIO ZAMBOLIM, Universidade Federal de Viçosa; VALDIR DIOLA, Universidade Federal de Viçosa; MARCELO ELHERS LOUREIRO, Universidade Federal de Viçosa.
Título: | Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene.
Ano de publicação: | 2010
Fonte/Imprenta: | EUPHYTICA, v. 173, p. 255-264. 2010.
Páginas: | 255-264
Idioma: | Inglês
Conteúdo: | The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype H&#305;´brido de Timor UFV 427-15 and the susceptible cultivar Catua&#305;´ Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of H&#305;´brido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT 300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in H&#305;´brido de Timor and can be useful for marker assisted selection in coffee breeding programs.
Palavras-Chave: | AFLP marker; Bulked segregant analysis; Coffee leaf rust; Resistance gene.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/29345/1/Inheritance-of-coffee-leaf.pdf
Registro original: | Embrapa Café (CNPCa)

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