|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
03/06/2023 |
Data da última atualização: |
22/08/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, C. S.; ROSA, P. M. da S.; ALVES, B. R. C.; MONTEIRO, C. A. S.; LEAL, G. R.; GUEDES, P. H. E.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; PAOLA MARIA DA SILVA ROSA; BRUNA RIOS COELHO ALVES; CLARA ANA SANTOS MONTEIRO; GABRIELA RAMOS LEAL; PEDRO HENRIQUE EVANGELISTA GUEDES; AGOSTINHO JORGE DOS REIS CAMARGO; NAIARA ZOCCAL SARAIVA, CNPGL. |
Título: |
Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Zygote, v. 31, n. 4, p. 342-349, 2023. |
DOI: |
https://doi.org/10.1017/S0967199423000230 |
Idioma: |
Inglês |
Conteúdo: |
Induction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV groups. In conclusion, our results demonstrate a lower LH secretion profile in peripubertal Gir heifers prone to ovulate after induction protocol, and that oocyte quality is not affected on a short-term basis by ovulation itself. MenosInduction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV group... Mostrar Tudo |
Palavras-Chave: |
Competência oocitária; Puberdade. |
Thesagro: |
Bovino. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02495naa a2200253 a 4500 001 2154200 005 2023-08-22 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199423000230$2DOI 100 1 $aOLIVEIRA, C. S. 245 $aLuteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers.$h[electronic resource] 260 $c2023 520 $aInduction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV groups. In conclusion, our results demonstrate a lower LH secretion profile in peripubertal Gir heifers prone to ovulate after induction protocol, and that oocyte quality is not affected on a short-term basis by ovulation itself. 650 $aBovino 653 $aCompetência oocitária 653 $aPuberdade 700 1 $aROSA, P. M. da S. 700 1 $aALVES, B. R. C. 700 1 $aMONTEIRO, C. A. S. 700 1 $aLEAL, G. R. 700 1 $aGUEDES, P. H. E. 700 1 $aCAMARGO, A. J. dos R. 700 1 $aSARAIVA, N. Z. 773 $tZygote$gv. 31, n. 4, p. 342-349, 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
16/10/2018 |
Data da última atualização: |
16/10/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GALEANO, R. M. S.; DURE, L. M. M.; OLIVEIRA, L. H. M. M.; CAMPELO, A. P. S.; PAGGI, G. M.; FERNANDES JUNIOR, P. I.; BALDANI, J. I.; BRASIL, M. S. |
Afiliação: |
UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; PAULO IVAN FERNANDES JUNIOR, CPATSA; JOSE IVO BALDANI, CNPAB; UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL. |
Título: |
Functional activities of Bacillus isolated from bromeliads and growth promoting activity of maize exposed to drought .stress |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16.; LATINAMERICAN WORKSHOP OF PGPR, 4.; RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Embrapa, 2018. |
Páginas: |
p. 104 |
Idioma: |
Inglês |
Notas: |
Sessio 6, Poster VI.82 |
Palavras-Chave: |
ACC deaminase. |
Thesaurus NAL: |
Germination; Seed inoculation. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 00895nam a2200241 a 4500 001 2097535 005 2018-10-16 008 2018 bl uuuu u01u1 u #d 100 1 $aGALEANO, R. M. S. 245 $aFunctional activities of Bacillus isolated from bromeliads and growth promoting activity of maize exposed to drought .stress$h[electronic resource] 260 $aIn: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16.; LATINAMERICAN WORKSHOP OF PGPR, 4.; RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Embrapa$c2018 300 $ap. 104 500 $aSessio 6, Poster VI.82 650 $aGermination 650 $aSeed inoculation 653 $aACC deaminase 700 1 $aDURE, L. M. M. 700 1 $aOLIVEIRA, L. H. M. M. 700 1 $aCAMPELO, A. P. S. 700 1 $aPAGGI, G. M. 700 1 $aFERNANDES JUNIOR, P. I. 700 1 $aBALDANI, J. I. 700 1 $aBRASIL, M. S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|