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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
13/01/2023 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, E. R.; FREIRE, R. P.; SUZUKI, M. F.; OLIVEIRA, J. E.; YOSIDAKI, V. L.; PERONI, C. N.; SEVILHANO, T.; ZORZETO, M.; TORATI, L. S.; SOARES, C. R. J.; LIMA, I. D. de M.; KRONENBERGER, T.; MALTAROLLO, V. G.; BARTOLINI, P. |
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Afiliação: |
ELIANA ROSA LIMA, IPEN-CNEN; RENAN PASSOS FREIRE, IPEN-CNEN; MIRIAM FUSSAE SUZUKI, IPEN-CNEN; JOÃO EZEQUIEL OLIVEIRA, IPEN-CNEN; VANESSA LUNA YOSIDAKI, IPEN-CNEN; CIBELE NUNES PERONI, IPEN-CNEN; THAÍS SEVILHANO, IPEN-CNEN; MOISÉS ZORZETO, PISCICULTURA RAÇA, MT; LUCAS SIMON TORATI, CNPASA; CARLOS ROBERTO JORGE SOARES, IPEN-CNEN; IGOR DANIEL DE MIRANDA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; THALES KRONENBERGER, Eberhard Karls University Tübingen, Germany; VINICIUS GONÇALVES MALTAROLLO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAOLO BARTOLINI, IPEN-CNEN. |
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Título: |
Isolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomolecules, v. 13, n. 1, art. 158, 2023. |
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ISSN: |
2218-273X |
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DOI: |
https://doi.org/10.3390/biom13010158 |
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Idioma: |
Inglês |
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Conteúdo: |
In a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies. |
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Palavras-Chave: |
Growth hormone; In silico sequencing. |
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Thesagro: |
Hormônio Animal; Peixe; Pirarucu. |
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Thesaurus Nal: |
Arapaima gigas; Hormones; Molecular models. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150987/1/biomolecules-2023.pdf
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Marc: |
LEADER 02661naa a2200397 a 4500
001 2150987
005 2023-02-13
008 2023 bl uuuu u00u1 u #d
022 $a2218-273X
024 7 $ahttps://doi.org/10.3390/biom13010158$2DOI
100 1 $aLIMA, E. R.
245 $aIsolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH).$h[electronic resource]
260 $c2023
520 $aIn a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies.
650 $aArapaima gigas
650 $aHormones
650 $aMolecular models
650 $aHormônio Animal
650 $aPeixe
650 $aPirarucu
653 $aGrowth hormone
653 $aIn silico sequencing
700 1 $aFREIRE, R. P.
700 1 $aSUZUKI, M. F.
700 1 $aOLIVEIRA, J. E.
700 1 $aYOSIDAKI, V. L.
700 1 $aPERONI, C. N.
700 1 $aSEVILHANO, T.
700 1 $aZORZETO, M.
700 1 $aTORATI, L. S.
700 1 $aSOARES, C. R. J.
700 1 $aLIMA, I. D. de M.
700 1 $aKRONENBERGER, T.
700 1 $aMALTAROLLO, V. G.
700 1 $aBARTOLINI, P.
773 $tBiomolecules$gv. 13, n. 1, art. 158, 2023.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
12/08/2016 |
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Data da última atualização: |
24/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BARLOW, J.; LENNOX, G. D.; FERREIRA, J.; BERENGUER, E.; LEES, A. C.; NALLY, R. M.; THOMSON, J. R.; FERRAZ, S. F. de B.; LOUZADA, J.; OLIVEIRA, V. H. F.; PARRY, L.; SOLAR, R. R. de C.; VIEIRA, I. C. G.; ARAGÃO, L. E. O. C.; BEGOTTI, R. A.; BRAGA, R. F.; CARDOSO, T. M.; OLIVEIRA JUNIOR, R. C. de; SOUZA JUNIOR, C. M.; MOURA, N. G.; NUNES, S. S.; SIQUEIRA, J. V.; PARDINI, R.; SILVEIRA, J. M.; VAZ-DE-MELLO, F. Z.; VEIGA, R. C. S.; VENTURIERI, A.; GARDNER, T. A. |
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Afiliação: |
Jos Barlow, Lancaster University / MPEG / UFLA; Gareth D. Lennox, Lancaster University; JOICE NUNES FERREIRA, CPATU; Erika Berenguer, Lancaster University; Alexander C. Lees, MPEG / Cornell University; Ralph Mac Nally, University of Canberra; James R. Thomson, University of Canberra / Arthur Rylah Institute for Environmental Research; Silvio Frosini de Barros Ferraz, USP / ESALQ; Julio Louzada, Lancaster University / UFLA; Victor Hugo Fonseca Oliveira, Lancaster University / UFLA; Luke Parry, Lancaster University / UFPA; Ricardo Ribeiro de Castro Solar, UFV; Ima C. G. Vieira, MPEG; Luiz E. O. C. Aragão, INPE / University of Exeter; Rodrigo Anzolin Begotti, USP / ESALQ; Rodrigo F. Braga, UFLA; Thiago Moreira Cardoso, COLABORADOR CPATU; RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; Carlos M. Souza Junior, IMAZON; Nárgila G. Moura, MPEG / Cornell University; Sâmia Serra Nunes, IMAZON; João Victor Siqueira, IMAZON; Renata Pardini, USP; Juliana M. Silveira, Lancaster University / UFLA; Fernando Z. Vaz-de-Mello, UFMT; Ruan Carlo Stulpen Veiga, Instituto Socio Ambiental Serra do Mar (ISASM); ADRIANO VENTURIERI, CPATU; Toby A. Gardner, Stockholm Environment Institute / International Institute for Sustainability. |
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Título: |
Anthropogenic disturbance in tropical forests can double biodiversity loss from deforestation. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Nature, v. 535, n. 7610, p. 144-147, July 2016. |
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DOI: |
10.1038/nature18326 |
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Idioma: |
Inglês |
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Conteúdo: |
Concerted political attention has focused on reducing deforestation1, 2, 3, and this remains the cornerstone of most biodiversity conservation strategies4, 5, 6. However, maintaining forest cover may not reduce anthropogenic forest disturbances, which are rarely considered in conservation programmes6. These disturbances occur both within forests, including selective logging and wildfires7, 8, and at the landscape level, through edge, area and isolation effects9. Until now, the combined effect of anthropogenic disturbance on the conservation value of remnant primary forests has remained unknown, making it impossible to assess the relative importance of forest disturbance and forest loss. Here we address these knowledge gaps using a large data set of plants, birds and dung beetles (1,538, 460 and 156 species, respectively) sampled in 36 catchments in the Brazilian state of Pará. Catchments retaining more than 69?80% forest cover lost more conservation value from disturbance than from forest loss. For example, a 20% loss of primary forest, the maximum level of deforestation allowed on Amazonian properties under Brazil?s Forest Code5, resulted in a 39?54% loss of conservation value: 96?171% more than expected without considering disturbance effects. We extrapolated the disturbance-mediated loss of conservation value throughout Pará, which covers 25% of the Brazilian Amazon. Although disturbed forests retained considerable conservation value compared with deforested areas, the toll of disturbance outside Pará?s strictly protected areas is equivalent to the loss of 92,000?139,000 km2 of primary forest. Even this lowest estimate is greater than the area deforested across the entire Brazilian Amazon between 2006 and 2015 (ref. 10). Species distribution models showed that both landscape and within-forest disturbances contributed to biodiversity loss, with the greatest negative effects on species of high conservation and functional value. These results demonstrate an urgent need for policy interventions that go beyond the maintenance of forest cover to safeguard the hyper-diversity of tropical forest ecosystems. MenosConcerted political attention has focused on reducing deforestation1, 2, 3, and this remains the cornerstone of most biodiversity conservation strategies4, 5, 6. However, maintaining forest cover may not reduce anthropogenic forest disturbances, which are rarely considered in conservation programmes6. These disturbances occur both within forests, including selective logging and wildfires7, 8, and at the landscape level, through edge, area and isolation effects9. Until now, the combined effect of anthropogenic disturbance on the conservation value of remnant primary forests has remained unknown, making it impossible to assess the relative importance of forest disturbance and forest loss. Here we address these knowledge gaps using a large data set of plants, birds and dung beetles (1,538, 460 and 156 species, respectively) sampled in 36 catchments in the Brazilian state of Pará. Catchments retaining more than 69?80% forest cover lost more conservation value from disturbance than from forest loss. For example, a 20% loss of primary forest, the maximum level of deforestation allowed on Amazonian properties under Brazil?s Forest Code5, resulted in a 39?54% loss of conservation value: 96?171% more than expected without considering disturbance effects. We extrapolated the disturbance-mediated loss of conservation value throughout Pará, which covers 25% of the Brazilian Amazon. Although disturbed forests retained considerable conservation value compared with deforested areas, the to... Mostrar Tudo |
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Palavras-Chave: |
Biologia da conservação; Ecologia tropical. |
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Thesagro: |
Ecologia Florestal; Floresta Tropical. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 03575naa a2200505 a 4500
001 2050817
005 2022-05-24
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1038/nature18326$2DOI
100 1 $aBARLOW, J.
245 $aAnthropogenic disturbance in tropical forests can double biodiversity loss from deforestation.$h[electronic resource]
260 $c2016
520 $aConcerted political attention has focused on reducing deforestation1, 2, 3, and this remains the cornerstone of most biodiversity conservation strategies4, 5, 6. However, maintaining forest cover may not reduce anthropogenic forest disturbances, which are rarely considered in conservation programmes6. These disturbances occur both within forests, including selective logging and wildfires7, 8, and at the landscape level, through edge, area and isolation effects9. Until now, the combined effect of anthropogenic disturbance on the conservation value of remnant primary forests has remained unknown, making it impossible to assess the relative importance of forest disturbance and forest loss. Here we address these knowledge gaps using a large data set of plants, birds and dung beetles (1,538, 460 and 156 species, respectively) sampled in 36 catchments in the Brazilian state of Pará. Catchments retaining more than 69?80% forest cover lost more conservation value from disturbance than from forest loss. For example, a 20% loss of primary forest, the maximum level of deforestation allowed on Amazonian properties under Brazil?s Forest Code5, resulted in a 39?54% loss of conservation value: 96?171% more than expected without considering disturbance effects. We extrapolated the disturbance-mediated loss of conservation value throughout Pará, which covers 25% of the Brazilian Amazon. Although disturbed forests retained considerable conservation value compared with deforested areas, the toll of disturbance outside Pará?s strictly protected areas is equivalent to the loss of 92,000?139,000 km2 of primary forest. Even this lowest estimate is greater than the area deforested across the entire Brazilian Amazon between 2006 and 2015 (ref. 10). Species distribution models showed that both landscape and within-forest disturbances contributed to biodiversity loss, with the greatest negative effects on species of high conservation and functional value. These results demonstrate an urgent need for policy interventions that go beyond the maintenance of forest cover to safeguard the hyper-diversity of tropical forest ecosystems.
650 $aEcologia Florestal
650 $aFloresta Tropical
653 $aBiologia da conservação
653 $aEcologia tropical
700 1 $aLENNOX, G. D.
700 1 $aFERREIRA, J.
700 1 $aBERENGUER, E.
700 1 $aLEES, A. C.
700 1 $aNALLY, R. M.
700 1 $aTHOMSON, J. R.
700 1 $aFERRAZ, S. F. de B.
700 1 $aLOUZADA, J.
700 1 $aOLIVEIRA, V. H. F.
700 1 $aPARRY, L.
700 1 $aSOLAR, R. R. de C.
700 1 $aVIEIRA, I. C. G.
700 1 $aARAGÃO, L. E. O. C.
700 1 $aBEGOTTI, R. A.
700 1 $aBRAGA, R. F.
700 1 $aCARDOSO, T. M.
700 1 $aOLIVEIRA JUNIOR, R. C. de
700 1 $aSOUZA JUNIOR, C. M.
700 1 $aMOURA, N. G.
700 1 $aNUNES, S. S.
700 1 $aSIQUEIRA, J. V.
700 1 $aPARDINI, R.
700 1 $aSILVEIRA, J. M.
700 1 $aVAZ-DE-MELLO, F. Z.
700 1 $aVEIGA, R. C. S.
700 1 $aVENTURIERI, A.
700 1 $aGARDNER, T. A.
773 $tNature$gv. 535, n. 7610, p. 144-147, July 2016.
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