Data/hora: 10/06/2024 02:40:57

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 01/12/1997
Data da última atualização: | 01/12/1997
Autoria: | BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W.
Título: | Molecular microbial diversity of an agricultural soil in Wisconsin.
Ano de publicação: | 1996
Fonte/Imprenta: | Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996.
Idioma: | Inglês
Conteúdo: | A culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were  cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots.
Thesagro: | Ecologia; Microrganismo; Solo.
Thesaurus Nal: | microbial ecology; soil; Wisconsin.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24346-1) - UPC - AP - -- -  -  - 22187 - DIS