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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
13/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANDRADE, J. de S.; MOREIRA, E. M.; SILVA, G. M. da; SCHNEIDER, A.; NUNES, V. R. R.; SILVA, R. R. da; PFEIFER, L. F. M. |
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Afiliação: |
JÉSSICA DE SOUZA ANDRADE, Bionorte, Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Porto Velho, RO; ELIZÂNGELA MÍRIAN MOREIRA, Universidade Federal do Amazonas (UFAM); GEORGE MOREIRA DA SILVA, Universidade Federal de Rondônia (UNIR); AUGUSTO SCHNEIDER, Universidade Federal de Pelotas (UFPel); VANESSA RACHELE RIBEIRO NUNES, CPAF-RO; RENATA REIS DA SILVA, CPAF-RO; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
Uterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 249, 104528, July 2021. |
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ISSN: |
1871-1413 |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104528 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both). MenosThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, trans... Mostrar Tudo |
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Palavras-Chave: |
Citocina; Cytobrush; Inseminação artifical por tempo determinado; PMN cells; Timed artificial insemination (TAI); Uterine health. |
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Thesagro: |
Fertilidade Animal; Gado de Corte; Gado Nelore; Imunidade; Pós-Parto; Reprodução Animal; Sanidade Animal; Útero; Vaca de Corte. |
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Thesaurus Nal: |
Animal fertility; Animal reproduction; Beef cows; Cytokines; Immunity; Nellore; Postpartum period. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03446naa a2200481 a 4500
001 2131781
005 2021-05-13
008 2021 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $ahttps://doi.org/10.1016/j.livsci.2021.104528$2DOI
100 1 $aANDRADE, J. de S.
245 $aUterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome.$h[electronic resource]
260 $c2021
520 $aThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both).
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aBeef cows
650 $aCytokines
650 $aImmunity
650 $aNellore
650 $aPostpartum period
650 $aFertilidade Animal
650 $aGado de Corte
650 $aGado Nelore
650 $aImunidade
650 $aPós-Parto
650 $aReprodução Animal
650 $aSanidade Animal
650 $aÚtero
650 $aVaca de Corte
653 $aCitocina
653 $aCytobrush
653 $aInseminação artifical por tempo determinado
653 $aPMN cells
653 $aTimed artificial insemination (TAI)
653 $aUterine health
700 1 $aMOREIRA, E. M.
700 1 $aSILVA, G. M. da
700 1 $aSCHNEIDER, A.
700 1 $aNUNES, V. R. R.
700 1 $aSILVA, R. R. da
700 1 $aPFEIFER, L. F. M.
773 $tLivestock Science$gv. 249, 104528, July 2021.
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Embrapa Rondônia (CPAF-RO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
26/10/2023 |
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Data da última atualização: |
21/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
BORGES, R. C. F.; LEITE, S. dos S.; MARTINS, J. M. T.; SANTOS, M. D. M.; VELOSO, J. S.; MADEIRA, N. R.; FONSECA, M. E. N.; BOITEUX, L. S.; REIS, A. |
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Afiliação: |
RAFAELA C. F. BORGES, UNIVERSIDADE DE BRASÍLIA; STÉFANI DOS SANTOS LEITE, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; JOÃO MARCOS T. MARTINS, UNIVERSIDADE DE BRASÍLIA; MARIA D. M. SANTOS, UNIVERSIDADE DE BRASÍLIA; JOSIENE S. VELOSO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; NUNO RODRIGO MADEIRA, CNPH; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; LEONARDO SILVA BOITEUX, CNPH; AILTON REIS, CNPH. |
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Título: |
Powdery mildew (Erysiphe cruciferarum) on brassicaceae in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Australasian Plant Pathology, v. 52, p. 419-425, 2023. |
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DOI: |
https://doi.org/10.1007/s13313-023-00930-y |
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Idioma: |
Inglês |
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Conteúdo: |
A fungal complex belonging to the order Helotiales (including Erysiphaceae) has been reported inducing powdery mildew (PM) on members of the Brassicaceae family worldwide. However, no formal report is available employing comprehensive taxonomic analyses of the PM-inducing fungi on these hosts in Brazil. Herein, PM isolates from six Brassicaceae vegetables (viz. broccoli, cauliflower, kale, Chinese cabbage, Indian mustard, and wild mustard) were collected in five geographic areas in the Brazilian Cerrado biome. Morphological aspects of hyphae, conidiophores, and conidia of these isolates were employed in combination with phylogenetic analyses of the sequences of the internal transcribed spacer (ITS) region of rDNA obtained with the pair of Erysiphaceae-specific primers (PMITS1 and PMITS2). Erysiphe cruciferarum was identified as the sole causal PM agent in all six Brassicaceae vegetables. Pathogenicity of the isolates was evaluated by spraying conidial suspension on Brassicaceae seedlings under greenhouse conditions. All isolates were pathogenic to their original hosts. PM has become an emergent foliar disease of Brassicaceae in Brazil especially in dry areas under dripping irrigation systems. This disease has been a limiting factor in the Cerrado region where a long and well-defined dry season favours disease onset and progression. |
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Thesagro: |
Brassica Juncea; Brassica Oleracea; Brassica Rapa; Etiologia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02159naa a2200277 a 4500
001 2157542
005 2024-02-21
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13313-023-00930-y$2DOI
100 1 $aBORGES, R. C. F.
245 $aPowdery mildew (Erysiphe cruciferarum) on brassicaceae in Brazil.$h[electronic resource]
260 $c2023
520 $aA fungal complex belonging to the order Helotiales (including Erysiphaceae) has been reported inducing powdery mildew (PM) on members of the Brassicaceae family worldwide. However, no formal report is available employing comprehensive taxonomic analyses of the PM-inducing fungi on these hosts in Brazil. Herein, PM isolates from six Brassicaceae vegetables (viz. broccoli, cauliflower, kale, Chinese cabbage, Indian mustard, and wild mustard) were collected in five geographic areas in the Brazilian Cerrado biome. Morphological aspects of hyphae, conidiophores, and conidia of these isolates were employed in combination with phylogenetic analyses of the sequences of the internal transcribed spacer (ITS) region of rDNA obtained with the pair of Erysiphaceae-specific primers (PMITS1 and PMITS2). Erysiphe cruciferarum was identified as the sole causal PM agent in all six Brassicaceae vegetables. Pathogenicity of the isolates was evaluated by spraying conidial suspension on Brassicaceae seedlings under greenhouse conditions. All isolates were pathogenic to their original hosts. PM has become an emergent foliar disease of Brassicaceae in Brazil especially in dry areas under dripping irrigation systems. This disease has been a limiting factor in the Cerrado region where a long and well-defined dry season favours disease onset and progression.
650 $aBrassica Juncea
650 $aBrassica Oleracea
650 $aBrassica Rapa
650 $aEtiologia
700 1 $aLEITE, S. dos S.
700 1 $aMARTINS, J. M. T.
700 1 $aSANTOS, M. D. M.
700 1 $aVELOSO, J. S.
700 1 $aMADEIRA, N. R.
700 1 $aFONSECA, M. E. N.
700 1 $aBOITEUX, L. S.
700 1 $aREIS, A.
773 $tAustralasian Plant Pathology$gv. 52, p. 419-425, 2023.
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