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| 9. |  | SIMINSKI, A.; MANTOVANI, M.; REIS, M. S. dos; FANTINI, A. C. Sucessão florestal secundária no município de São Pedro de Alcântara, litoral de Santa Catarina: estrutura e diversidade. Ciência Florestal, Santa Maria, v. 14, n. 1, p. 21-33, jun. 2004.| Biblioteca(s): Embrapa Florestas. |
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| 10. | | SILVA, F. A. L. S. da; BITTENCOURT, R.; FERREIRA, D. K.; SIMINSKI, A.; PUCHALKI, A.; REIS, M. S. dos. Caracterização do incremento diamétrico de Araucaria angustifolia (Bert.) O. Kuntze na Floresta Nacional de Três Barras, SC. In: SEMINÁRIO DE PESQUISA DA FLORESTA NACIONAL DE TRÊS BARRAS, 1., 2009, Três Barras. Uso múltiplo e sustentável dos recursos florestais e conservação da natureza: anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. Resumo expandido.| Biblioteca(s): Embrapa Florestas. |
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| 11. | | SIMINSKI, A.; RUSCHEL, A. R.; FERREIRA, D. K.; BITTENCOURT, R.; MARIOT, A.; MATTOS, A. G.; SILVA, C. V. da; SILVA, J. Z. da; SCHULTZ, J.; MARQUES, A. da C.; REIS, M. S. dos. Caracterização da cobertura vegetal nativa da Floresta Nacional de Três Barras, SC. In: SEMINÁRIO DE PESQUISA DA FLORESTA NACIONAL DE TRÊS BARRAS, 1., 2009, Três Barras. Uso múltiplo e sustentável dos recursos florestais e conservação da natureza: anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. Resumo expandido.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/03/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ZANCANELA, D. C.; FUNARI, C. S.; HERCULANO, R. D.; MELLO, V. M.; RODRIGUES, C. M.; BORGES, F. A.; BARROS, N. R. de; MARCOS, C. M.; ALMEIDA, A. M. F.; GUASTALDI, A. C. |
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Afiliação: |
Daniela Cervelle Zancanela; Cristiano Soleo Funari; Rondinelli Donizetti Herculano; Vinicius Moreira Mello; CLENILSON MARTINS RODRIGUES, CNPAE; Felipe Azevedo Borges; Natan Roberto de Barros; Caroline Maria Marcos; Ana Marisa Fusco Almeida; Antônio Carlos Guastaldi. |
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Título: |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Materials Science & Engineering C, v. 97, p. 576-582, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV?Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing. MenosNatural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV?Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate aft... Mostrar Tudo |
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Palavras-Chave: |
Antifungal; Propolis release; Red propolis; Skin wound healing. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02807naa a2200277 a 4500
001 2107124
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aZANCANELA, D. C.
245 $aNatural rubber latex membranes incorporated with three different types of propolis$bPhysical-chemistry and antimicrobial behaviours.$h[electronic resource]
260 $c2019
520 $aNatural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV?Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.
653 $aAntifungal
653 $aPropolis release
653 $aRed propolis
653 $aSkin wound healing
700 1 $aFUNARI, C. S.
700 1 $aHERCULANO, R. D.
700 1 $aMELLO, V. M.
700 1 $aRODRIGUES, C. M.
700 1 $aBORGES, F. A.
700 1 $aBARROS, N. R. de
700 1 $aMARCOS, C. M.
700 1 $aALMEIDA, A. M. F.
700 1 $aGUASTALDI, A. C.
773 $tMaterials Science & Engineering C$gv. 97, p. 576-582, 2019.
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