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| Registros recuperados : 12 | |
| 4. |  | BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. Combination of ethylene glycol with sucrose increases survival rate after vitrification of somatic tissue of collared peccaries (Pecari tajacu Linnaeus, 1758). Pesquisa Veterinária Brasileira, Rio de Janeiro, V. 3, n. 2, p. 350-356, fevereiro 2018. Título em português: Combinação de etilenoglicol com sacarose aumenta a taxa de sobrevivência após a vitrificação de tecido somático de catetos (Pecari tajacu Linnaeus, 1758).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | SILVA JÚNIOR, M. S. F. S. da; BORGES, A. A. de S.; SANTOS, S. R. N.; MOURA, V. G. de; SILVA, G. R. da; ARAUJO, A. M. de. Eficiência de protocolos para extração de DNA com bulbos e sangue de galinhas caipiras. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 4., 2018, Teresina, PI. Anais da IV Jornada Científica da Embrapa Meio-Norte. Brasília, DF: Embrapa, 2018. p. 36.| Biblioteca(s): Embrapa Meio-Norte. |
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| 6. | | BORGES, A. A. de S.; SILVA JÚNIOR, M. S. F. S. da; OLIVEIRA, R. de S.; LEDUR, M. C.; ALMEIDA, M. J. de O; ARAÚJO, A. M. de. Descritores morfológicos de galinhas da sub-região Meio-norte do Brasil. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020. Trabalho 114. Evento online.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Suínos e Aves. |
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| 7. | | SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | BORGES, A. A.; SANTOS, M. V. O.; QUEIROZ NETA, L. B.; OLIVEIR, A M. F.; SILVA, A. R.; PEREIRA, A. F. In vitro maturation of collared peccary (Pecari tajacu) oocytes after different incubation times. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 9, p. 1863-1868, setembro 2018 Título em português: Maturação in vitro de oócitos de cateto (Pecari tajacu) após diferentes períodos de incubação.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | SILVA JÚNIOR, M. S. F. S. da; BORGES, A. A. de S.; SANTOS, S. R. N.; MOURA, V. G. de; DIAS, A. C. S.; SILVA, G. R. da; ARAUJO, A. M. de; OLIVEIRA FILHO, J. W. G. de. Caracterização genética de galinhas (Gallus gallus domesticus) de comunidades rurais do Meio-Norte do Brasil. In: OELKE, C. A. (Org.). Suinocultura e Avicultura: do básico a zootecnia de precisão. Guarujá, SP: Científica Digital, 2021. Cap. 7. p. 116-128.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Pantanal. |
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| 10. | | OLIVEIRA, R. S.; SILVA, G. R. da; BORGES, A. A. de S.; MACHADO, L. P. M.; ARRÉ, F. A.; ARAÚJO, A. M. de; DINIZ, F. M.; SARMENTO, L. R.; SILVA, V. C. A Miostatina no melhoramento genético animal para a produção de carne. Archivos de Zootecnia, v. 72, n. 277, p. 78-84, 2023. Título em inglês: Myostatin in animal genetic improvement for meat production.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pantanal. |
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| 11. | | SILVA JUNIOR, M. S. F. S. da; BORGES, A. A. de S.; SANTOS, S. R. N.; MOURA, V. G. de; SILVANA, G. R. da; DIAS, A. C. S.; ARAUJO, A. M. de; OLIVEIRA FILHO, J. W. G. de. SSR and ISSR markers in assessing genetic diversity in Gallus gallus domesticus: a quantitative analysis of scientific production. Ciência Rural, v. 50, N. 7, e20190401, 2020.| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | SANTOS, S. R. N.; SILVA JÚNIOR, M. S. F. S. da; BORGES, A. A. de S.; MOURA, V. G. de; OLIVEIRA FILHO, J. W. G. de; ARAUJO, A. M. de. Utilização dos marcadores moleculares SSR e ISSR no estudo de diversidade genética em galinhas caipiras: uma revisão sistemática. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 4., 2018, Teresina, PI. Anais da IV Jornada Científica da Embrapa Meio-Norte. Brasília, DF: Embrapa, 2018. p. 57.| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 12 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. |
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Afiliação: |
MAGDA L. T. SANTOS, UFERSA; ALANA A. BORGES, UFERSA; LUIZA B. QUEIROZ NETA, UFERSA; MARIA V. O. SANTOS, UFERSA; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA; ALEXANDRA F. PEREIRA, UFERSA. |
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Título: |
In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016. |
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Idioma: |
Português |
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Conteúdo: |
The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries. MenosThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve th... Mostrar Tudo |
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Palavras-Chave: |
Mitotic factor; Somatic tissue. |
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Thesagro: |
Tayassu Tajacu. |
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Thesaurus NAL: |
Culture media; Protein sources; Somatic cells; Wild animals. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155804/1/In-vitro-culutre-of-somatic.pdf
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Marc: |
LEADER 02511naa a2200277 a 4500
001 2064367
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. L. T.
245 $aIn vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.$h[electronic resource]
260 $c2016
520 $aThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
650 $aCulture media
650 $aProtein sources
650 $aSomatic cells
650 $aWild animals
650 $aTayassu Tajacu
653 $aMitotic factor
653 $aSomatic tissue
700 1 $aBORGES, A. A.
700 1 $aQUEIROZ NETA, L. B.
700 1 $aSANTOS, M. V. O.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
700 1 $aPEREIRA, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1194-1202, dez. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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