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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Florestas. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
13/03/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. |
Afiliação: |
Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF. |
Título: |
Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. |
DOI: |
10.1007/s11676-018-0777-4 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. |
Palavras-Chave: |
Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética. |
Thesagro: |
Eucalipto; Eucalyptus Urophylla. |
Thesaurus Nal: |
Genetic transformation; In vitro regeneration; Somaclonal variation. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02181naa a2200313 a 4500 001 2114265 005 2020-03-13 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-018-0777-4$2DOI 100 1 $aBETTENCOURT, G. M. de F. 245 $aAgrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.$h[electronic resource] 260 $c2020 520 $aAbstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. 650 $aGenetic transformation 650 $aIn vitro regeneration 650 $aSomaclonal variation 650 $aEucalipto 650 $aEucalyptus Urophylla 653 $aPlant growth regulators 653 $aRegeneração in vitro 653 $aReguladores de crescimento de plantas 653 $aTransformação genética 700 1 $aSOCCOL, C. R. 700 1 $aGIOVANELLA, T. S. 700 1 $aFRANCISCON, L. 700 1 $aKESTRING, D. R. 700 1 $aGERHARDT, I. R. 700 1 $aDEGENHARDT-GOLDBACH, J. 773 $tJournal of Forestry Research$gv. 31, n. 2, p. 507-519, Apr. 2020.
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1. | | ALVES, A. L.; BORBA, R. S. de; POZZOBON, A. P.; OLIVEIRA, C.; NIRCHIO, M.; FORESTI, F. Localization of 18S ribosomal genes in suckermouth armoured catfishes Loricariidae (Teleostei, Siluriformes) with discussion on the Ag-NOR evolution. Comparative Cytogenetics, Sofia, v. 6, n. 3, p. 315-321, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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2. | | ALVES, A. L.; BORBA, R. S. de; OLIVEIRA, C.; NIRCHIO, M.; GRANADO, A.; FORESTI, F. Karyotypic diversity and evolutionary trends in the Neotropical catfish genus Hypostomus Lacépède, 1803 (Teleostei, Siluriformes, Loricariidae). Comparative Cytogenetics, Sofia, v. 6, n. 4, p. 443-452, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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3. | | BORBA, R. S. de; ZAWADZKI, C. H.; OLIVEIRA, C.; PERDICES, A.; PARISE-MALTEMPI, P. P.; ALVES, A. L. Phylogeography of Hypostomus strigaticeps (Siluriformes: Loricariidae) inferred by mitochondrial DNA reveals its distribution in the upper Paraná River basin. Neotropical Ichthyology, Porto Alegre, v. 11, n. 1, p. 111-116, jan./mar. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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4. | | BORBA, R. S. de; SILVA, E. L. da; PACHECO, A. C. S.; PARISE-MALTEMPI, P. P.; ALVES, A. L. Trends in the karyotypic evolution of the Neotropical catfish family Heptapteridae Bockmann 1998 (Teleostei: Siluriformes). Reviews in Fish Biology and Fisheries, London, v. 22, n. 2, p. 509-518, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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5. | | BORBA, R. S. de; SILVA, E. L. da; PONZETTO, J. M.; POZZOBON, A. P. B.; CENTOFANTE, L.; ALVES, A. L.; PARISE-MALTEMPI, P. P. Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences. Biota Neotropica, São Paulo, v. 13, n. 1, p. 42-46, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
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