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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
01/12/2004 |
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Data da última atualização: |
01/12/2004 |
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Autoria: |
BEZERRA NETO, F.; BARROS JÚNIOR, A. P.; SILVA, E. de O. S.; NEGREIROS, M. Z. de; OLIVEIRA, E. Q. de; SILVEIRA, L. M. da; LIMA, J. S. S. de; FREITAS, K. K. C. de. |
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Título: |
Avaliação nutricional da cenoura em sistemas consorciados com alface em bicultivo sob diferentes densidades populacionais das culturas componentes. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. |
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Idioma: |
Português |
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Notas: |
Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 434-435, jul. 2004. Suplemento 1. |
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Palavras-Chave: |
Análise nutricional; Consorciação; Densidade populacional; Valor nutricional. |
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Thesagro: |
Alface; Cenoura; Daucus Carota; Lactuca Sativa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01140naa a2200301 a 4500
001 1777269
005 2004-12-01
008 2004 bl uuuu u00u1 u #d
100 1 $aBEZERRA NETO, F.
245 $aAvaliação nutricional da cenoura em sistemas consorciados com alface em bicultivo sob diferentes densidades populacionais das culturas componentes.
260 $c2004
500 $aTrabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 434-435, jul. 2004. Suplemento 1.
650 $aAlface
650 $aCenoura
650 $aDaucus Carota
650 $aLactuca Sativa
653 $aAnálise nutricional
653 $aConsorciação
653 $aDensidade populacional
653 $aValor nutricional
700 1 $aBARROS JÚNIOR, A. P.
700 1 $aSILVA, E. de O. S.
700 1 $aNEGREIROS, M. Z. de
700 1 $aOLIVEIRA, E. Q. de
700 1 $aSILVEIRA, L. M. da
700 1 $aLIMA, J. S. S. de
700 1 $aFREITAS, K. K. C. de
773 $tHorticultura Brasileira, Brasília$gv. 22, n. 2, jul. 2004. Suplemento 2.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
01/07/2003 |
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Data da última atualização: |
04/07/2023 |
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Autoria: |
MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAUJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. |
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Afiliação: |
CLAUDIO ROBERTO MADRUGA, CNPGC; CÁSSIA R. B. LEAL, UNIVERSIDADE CATÓLICA DOM BOSCO; ALDA M. T. FERREIRA, UNIVERSIDADE CATÓLICA DOM BOSCO; FLABIO RIBEIRO DE ARAUJO, CNPGC; ANA LIDIA VARIANI BONATO, CNPGC; RAUL HENRIQUE KESSLER, CNPGC; MARIA APARECIDA MOREIRA SCHENK, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC. |
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Título: |
Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 22, n. 4, p. 153-160, out./dez. 2002. |
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DOI: |
https://doi.org/10.1590/S0100-736X2002000400005 |
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Idioma: |
Inglês |
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Conteúdo: |
A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2. MenosA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surfac... Mostrar Tudo |
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Thesagro: |
Antígeno; Babesia Bigemina; Polimorfismo Genético. |
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Thesaurus NAL: |
Antigens; Genetic polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/253106/1/Genetic-antigenic-analysis-2002.pdf
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Marc: |
LEADER 03037naa a2200277 a 4500
001 1325288
005 2023-07-04
008 2002 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0100-736X2002000400005$2DOI
100 1 $aMADRUGA, C. R.
245 $aGenetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil.
260 $c2002
520 $aA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.
650 $aAntigens
650 $aGenetic polymorphism
650 $aAntígeno
650 $aBabesia Bigemina
650 $aPolimorfismo Genético
700 1 $aLEAL, C. R. B.
700 1 $aFERREIRA, A. M. T.
700 1 $aARAUJO, F. R.
700 1 $aBONATO, A. L. V.
700 1 $aKESSLER, R. H.
700 1 $aSCHENK, M. A. M.
700 1 $aSOARES, C. O.
773 $tPesquisa Veterinária Brasileira$gv. 22, n. 4, p. 153-160, out./dez. 2002.
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