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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
15/07/2009 |
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Data da última atualização: |
26/06/2012 |
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Autoria: |
CORAZZA-NUNESI; MACHADO, M. A.; STACH-MACHADO, D. R.; NUNES, W. M. C.; CARVALHO, S. A. de; MÜLLERV, G. W. |
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Afiliação: |
MARIA JÚLIA CORAZZA-NUNES, Depto. de Biologia, NBA/UEM; MARCOS ANTONIO MACHADO, C.C.S.M.-IAC; DAGMAR RUTH STACH-MACHADO, Depto. de Imunologia/UNICAMP; WILLIAM MÁRIO CARVALHO NUNES, Depto. de Agronomia, NBA/UEM; SÉRGIO ALVES DE CARVALHO, C.C.S.M.-IAC; GERD WALTER MÜLLER, NBA/UEM. |
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Título: |
Characterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of Citrus Active Germplasm Bank. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 32, n. 4, p. 322-327, Oct./Dec. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms. MenosCitrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or mo... Mostrar Tudo |
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Palavras-Chave: |
Anticorpos monoclonais; Citrus paradisi Macf; Sintomas de caneluras; SSCP; Vírus da tristeza dos citrus. |
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Thesagro: |
Elisa. |
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Thesaurus Nal: |
Enzyme-linked immunosorbent assay. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02384naa a2200265 a 4500
001 1083049
005 2012-06-26
008 2006 bl uuuu u00u1 u #d
100 1 $aCORAZZA-NUNESI
245 $aCharacterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of Citrus Active Germplasm Bank.
260 $c2006
520 $aCitrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms.
650 $aEnzyme-linked immunosorbent assay
650 $aElisa
653 $aAnticorpos monoclonais
653 $aCitrus paradisi Macf
653 $aSintomas de caneluras
653 $aSSCP
653 $aVírus da tristeza dos citrus
700 1 $aMACHADO, M. A.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aNUNES, W. M. C.
700 1 $aCARVALHO, S. A. de
700 1 $aMÜLLERV, G. W.
773 $tSumma Phytopathologica, Botucatu$gv. 32, n. 4, p. 322-327, Oct./Dec. 2006.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
31/03/2017 |
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Data da última atualização: |
24/04/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KUMODE, M. M. N.; BOLZON DE MUNIZ, G. I.; MAGALHAES, W. L. E.; KESTUR, S. G. |
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Afiliação: |
MARINA MIEKO NISHIDATE KUMODE, Pontifícia, Universidade Católica; GRACIELA I. MUNIZ BOLZON, Departamento de Engenharia e Tecnologia Florestal, UFPR; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; SATYANARAYANA G. KESTUR, Poornaprajna Institute of Scientific Research (PPISR). |
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Título: |
Microfibrillated nanocellulose from balsa tree as potential reinforcement in the preparation of 'green' composites with castor seed cake. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Cleaner Production, v. 149, p. 1157-1163, Apr. 2017. |
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DOI: |
http://dx.doi.org/10.1016/j.jclepro.2017.02.083 |
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Idioma: |
Inglês |
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Conteúdo: |
The search for and development of eco-friendly materials such as 'green' composites results from twin issues of sustainability and ?environmental impact?. Towards achieving this goal, the use of industrial residues such as plant fibers as reinforcement along with natural polymers has been yielding good results. However, due to limitations of industrial uptake of these materials in structural applications, reducing the size of fibers below the normal micro level offers advantages because of the improved behavior and functionality exhibited by the nano-sized cellulosic fibrous materials. Considering successful previous efforts to develop biodegradable composites using castor seed cake, this paper focuses on the possibility of developing ?green? composites using this material as matrix and nanocellulose prepared from balsa tree as reinforcement. The preparation of nanofibrillar cellulose through mechanical fibrillation and its characterization as well as preparation of nanocomposites by thermo-molding with a matrix plasticized by glycerin are presented. The incorporation of nanocellulose resulted in increased flexural modulus, swelling thickness, and water absorption with increasing addition of nanocellulose, while both apparent density and flexural strength remained unchanged. The observed properties are discussed based on fractographic studies. |
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Palavras-Chave: |
Flexural strength; Nanocompósito; Tecnologia sustentável; Thermo-molding. |
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Thesaurus NAL: |
castor bean cake; Nanocomposites; Sustainable technology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02228naa a2200253 a 4500
001 2067968
005 2017-04-24
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jclepro.2017.02.083$2DOI
100 1 $aKUMODE, M. M. N.
245 $aMicrofibrillated nanocellulose from balsa tree as potential reinforcement in the preparation of 'green' composites with castor seed cake.$h[electronic resource]
260 $c2017
520 $aThe search for and development of eco-friendly materials such as 'green' composites results from twin issues of sustainability and ?environmental impact?. Towards achieving this goal, the use of industrial residues such as plant fibers as reinforcement along with natural polymers has been yielding good results. However, due to limitations of industrial uptake of these materials in structural applications, reducing the size of fibers below the normal micro level offers advantages because of the improved behavior and functionality exhibited by the nano-sized cellulosic fibrous materials. Considering successful previous efforts to develop biodegradable composites using castor seed cake, this paper focuses on the possibility of developing ?green? composites using this material as matrix and nanocellulose prepared from balsa tree as reinforcement. The preparation of nanofibrillar cellulose through mechanical fibrillation and its characterization as well as preparation of nanocomposites by thermo-molding with a matrix plasticized by glycerin are presented. The incorporation of nanocellulose resulted in increased flexural modulus, swelling thickness, and water absorption with increasing addition of nanocellulose, while both apparent density and flexural strength remained unchanged. The observed properties are discussed based on fractographic studies.
650 $acastor bean cake
650 $aNanocomposites
650 $aSustainable technology
653 $aFlexural strength
653 $aNanocompósito
653 $aTecnologia sustentável
653 $aThermo-molding
700 1 $aBOLZON DE MUNIZ, G. I.
700 1 $aMAGALHAES, W. L. E.
700 1 $aKESTUR, S. G.
773 $tJournal of Cleaner Production$gv. 149, p. 1157-1163, Apr. 2017.
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