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| 112. | | GARCIA, F. R. M.; CARABAGIALLE, M. C.; SÁ, L. A. N. de; CAMPOS, J. V. Parasitismo natural de Phyllocnistis citrella Stainton, 1856 (Lepidoptera, Gracillariidae, Phyllocnistinae) no oeste de Santa Catarina, Brasil. Revista Brasileira de Entomologia, São Paulo, v. 45, n. 2, p. 139-143, 2001.| Biblioteca(s): Embrapa Meio Ambiente. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
06/01/2012 |
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Data da última atualização: |
11/04/2018 |
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Autoria: |
WEBER, R. L. M.; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
Ricardo Luís Mayer Weber, Universidade Federal do Rio Grande do Sul, Departamento de Genética; Maria Helena Bodanese?Zanettini, Universidade Federal do Rio Grande do Sul, Departamento de Genética. |
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Título: |
Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes-mediated transformation. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 9, p. 1070-1075, set. 2011 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Indução de raízes fasciculadas transgênicas em genótipos de soja por transformação mediada por Agrobacterium rhizogenes. |
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Conteúdo: |
The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes-mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS-5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four-day-old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S-GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency. Using an axenic in vitro protocol, excised cotyledons from four-day-old seedlings were infected with A. rhizogenes harboring the pCAMBIA1301 plasmid, containing the gusA reporter gene. The transformation frequency and the number of days for hairy root emergence after bacterial infection (DAI) were evaluated. The transformation frequency and DAI varied according to the genotype. Cultivars MG/BR 46 Conquista and BRSMG 68 Vencedora are more susceptible to A. rhizogenes and can be recommended for transformation experiments. |
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Palavras-Chave: |
Cultura de raíz fasciculada; Hairy roots culture; Planta composta; Strain K599; Transformação genética. |
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Thesagro: |
Glycine Max. |
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Thesaurus NAL: |
genetic transformation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51818/1/46n09a14.pdf
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Marc: |
LEADER 02170naa a2200229 a 4500
001 1911915
005 2018-04-11
008 2011 bl uuuu u00u1 u #d
100 1 $aWEBER, R. L. M.
245 $aInduction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes-mediated transformation.
260 $c2011
500 $aTítulo em português: Indução de raízes fasciculadas transgênicas em genótipos de soja por transformação mediada por Agrobacterium rhizogenes.
520 $aThe objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes-mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS-5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four-day-old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S-GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency. Using an axenic in vitro protocol, excised cotyledons from four-day-old seedlings were infected with A. rhizogenes harboring the pCAMBIA1301 plasmid, containing the gusA reporter gene. The transformation frequency and the number of days for hairy root emergence after bacterial infection (DAI) were evaluated. The transformation frequency and DAI varied according to the genotype. Cultivars MG/BR 46 Conquista and BRSMG 68 Vencedora are more susceptible to A. rhizogenes and can be recommended for transformation experiments.
650 $agenetic transformation
650 $aGlycine Max
653 $aCultura de raíz fasciculada
653 $aHairy roots culture
653 $aPlanta composta
653 $aStrain K599
653 $aTransformação genética
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPesquisa agropecuária Brasileira, Brasília, DF$gv. 46, n. 9, p. 1070-1075, set. 2011
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