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| 2. |  | GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M. Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis BMC Genomics, v. 15, n. 643, 2014.| Biblioteca(s): Embrapa Soja. |
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| 3. | | GODOY, R. L. de O.; CASTRO, I. M. de; ROSA, J. S. da; OLIVEIRA, E. M. M.; FREITAS, S. C. de; NOGUEIRA, A. R. A.; FERRACINI, V. L.; BLOCH, C.; NUNES, M. L. Consolidação de laboratórios multidisciplinares como centros colaboradores em defesa agropecuária. In: CONFERÊNCIA NACIONAL SOBRE DEFESA AGROPECUÁRIA, 2., 2010, Belo Horizonte. Mostra Tecnológica "Mais Ciência, Mais Tecnologia". Viçosa, MG: UFV, 2010.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Meio Ambiente; Embrapa Pecuária Sudeste. |
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| 4. | | RAMOS, H. J. O.; ANDRADE, C. A.; RIZZO, L. E.; MELO, J. A. T.; MAGALHÃES, D. M.; HUERGO, L.; C. W. GALVÃO; PEREIRA, L. F. P.; MARRACCINI, P.; CRUZ, L. M.; SOUZA, E. M.; BLOCH, C. J.; PEDROSA, F. O.; VIEIRA, L. G. E. Differential protein expression analysis of Coffea canephora plants under drought stress. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/08/2014 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M. |
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Afiliação: |
DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, v. 15, n. 643, 2014. |
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DOI: |
10.1186/1471-2164-15-643 |
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Idioma: |
Português |
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Conteúdo: |
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. MenosStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics... Mostrar Tudo |
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Palavras-Chave: |
RT-qPCR; Two-dimensional proteomics. |
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Thesaurus NAL: |
Bradyrhizobium; nitrogen fixation; symbiosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160837/1/12864-2014-Article-6775.pdf
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Marc: |
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100 1 $aGOMES, D. F.
245 $aProteomic analysis of free-living Bradyrhizobium diazoefficiens$bhighlighting potential determinants of a successful symbiosis$h[electronic resource]
260 $c2014
520 $aStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.
650 $aBradyrhizobium
650 $anitrogen fixation
650 $asymbiosis
653 $aRT-qPCR
653 $aTwo-dimensional proteomics
700 1 $aBATISTA, J. S. da S.
700 1 $aROLLA, A. A. P.
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700 1 $aBLOCH, C.
700 1 $aGALLI-TARASAWA, L. V.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics$gv. 15, n. 643, 2014.
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Embrapa Soja (CNPSO) |
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