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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/02/1997 |
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Data da última atualização: |
18/07/2018 |
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Autoria: |
VALLE, E. R. do. |
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Afiliação: |
University of Illinois at Urbana-Champaign. |
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Título: |
Methods to induce and synchronize ovulation and to enhance corpus luteum function and fertility in beef females. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Urbana: University of Illinois, 1986. |
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Páginas: |
102 p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
In the first section, a competitive inhibition double antibody enzyme immunoassay was developed using a horseradish peroxidase labelled progesterone conjungate and polystyrene tubes. Polystyrene tubes (12 x 75 mm) were incubated overnight at room temperature with 300 ul of sheep anti-rabbit IgG and washed 3 times with distilled water (1 ml) the next day. Standards or extracted samples in a phosphate buffer (100 ul), conjugate (100 ul) and rabbit anti-progesterone (100 ul) were added in that order to the tubes and incubated on a rotator at room temperature for 3 hours. Tubes were then washed 3 times with distilled water, 400 ul of O-phenylenediamine (16.8 mM) added and incubated in the dark for 30 minutes. After the addition of 400 ul of sulfuric acid (IN) absorbance at 492 nm was determined. The sheep anti-rabbit IgG was generated with Cohn Fraction II rabbit IgG as the immunogen and purified with ammonium sulfate. The rabbit anti-progesterone antibody was developed against 11 alpha-hydroxy-4-pregnene-3,20-dione-11- hemisuccinate: bovine serum albumin. The cross reactivity was greater than 2% only for 11 alpha-hydroxyprogesterone (22.0%) and 5 alpha-pregnan-3,20-dione (4.0%). The sensitivity of the assay was 5 pg (P<.05) and 84% of the progesterone spiked to samples was recovered which was similar to recovery of tritiated progesterone spiked samples (83%). When volumes of 50, 100, 150 and 200 ul of plasma were assayed, progesterone values were 3.63, 3.61, 3.56 and 3.61 (r = .99; P<.01). Intraassay and... MenosIn the first section, a competitive inhibition double antibody enzyme immunoassay was developed using a horseradish peroxidase labelled progesterone conjungate and polystyrene tubes. Polystyrene tubes (12 x 75 mm) were incubated overnight at room temperature with 300 ul of sheep anti-rabbit IgG and washed 3 times with distilled water (1 ml) the next day. Standards or extracted samples in a phosphate buffer (100 ul), conjugate (100 ul) and rabbit anti-progesterone (100 ul) were added in that order to the tubes and incubated on a rotator at room temperature for 3 hours. Tubes were then washed 3 times with distilled water, 400 ul of O-phenylenediamine (16.8 mM) added and incubated in the dark for 30 minutes. After the addition of 400 ul of sulfuric acid (IN) absorbance at 492 nm was determined. The sheep anti-rabbit IgG was generated with Cohn Fraction II rabbit IgG as the immunogen and purified with ammonium sulfate. The rabbit anti-progesterone antibody was developed against 11 alpha-hydroxy-4-pregnene-3,20-dione-11- hemisuccinate: bovine serum albumin. The cross reactivity was greater than 2% only for 11 alpha-hydroxyprogesterone (22.0%) and 5 alpha-pregnan-3,20-dione (4.0%). The sensitivity of the assay was 5 pg (P<.05) and 84% of the progesterone spiked to samples was recovered which was similar to recovery of tritiated progesterone spiked samples (83%). When volumes of 50, 100, 150 and 200 ul of plasma were assayed, progesterone values were 3.63, 3.61, 3.56 and 3.61 (r =... Mostrar Tudo |
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Palavras-Chave: |
Beef cow; Calf fertility; Novilha; Pos parto; Postpartum. |
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Thesagro: |
Fertilidade; Gado de Corte; Inseminação; Reprodução Animal. |
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Thesaurus Nal: |
insemination; reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02255nam a2200265 a 4500
001 1790833
005 2018-07-18
008 1986 bl uuuu m 00u1 u #d
100 1 $aVALLE, E. R. do
245 $aMethods to induce and synchronize ovulation and to enhance corpus luteum function and fertility in beef females.
260 $aUrbana: University of Illinois$c1986
300 $a102 p.
500 $aPh.D. Thesis.
520 $aIn the first section, a competitive inhibition double antibody enzyme immunoassay was developed using a horseradish peroxidase labelled progesterone conjungate and polystyrene tubes. Polystyrene tubes (12 x 75 mm) were incubated overnight at room temperature with 300 ul of sheep anti-rabbit IgG and washed 3 times with distilled water (1 ml) the next day. Standards or extracted samples in a phosphate buffer (100 ul), conjugate (100 ul) and rabbit anti-progesterone (100 ul) were added in that order to the tubes and incubated on a rotator at room temperature for 3 hours. Tubes were then washed 3 times with distilled water, 400 ul of O-phenylenediamine (16.8 mM) added and incubated in the dark for 30 minutes. After the addition of 400 ul of sulfuric acid (IN) absorbance at 492 nm was determined. The sheep anti-rabbit IgG was generated with Cohn Fraction II rabbit IgG as the immunogen and purified with ammonium sulfate. The rabbit anti-progesterone antibody was developed against 11 alpha-hydroxy-4-pregnene-3,20-dione-11- hemisuccinate: bovine serum albumin. The cross reactivity was greater than 2% only for 11 alpha-hydroxyprogesterone (22.0%) and 5 alpha-pregnan-3,20-dione (4.0%). The sensitivity of the assay was 5 pg (P<.05) and 84% of the progesterone spiked to samples was recovered which was similar to recovery of tritiated progesterone spiked samples (83%). When volumes of 50, 100, 150 and 200 ul of plasma were assayed, progesterone values were 3.63, 3.61, 3.56 and 3.61 (r = .99; P<.01). Intraassay and...
650 $ainsemination
650 $areproduction
650 $aFertilidade
650 $aGado de Corte
650 $aInseminação
650 $aReprodução Animal
653 $aBeef cow
653 $aCalf fertility
653 $aNovilha
653 $aPos parto
653 $aPostpartum
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Registro original: |
Embrapa Pantanal (CPAP) |
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