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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
03/05/2024 |
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Data da última atualização: |
03/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, S. S. L.; LOPES, J. M. L.; MATOS, E. M. de; CABRAL, E. G.; AZEVEDO, A. L. S.; MACHADO, M. A.; CAMPOS, J. M. S. de; MENINI NETO, L.; VICCINI, L. F. |
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Afiliação: |
SHAIANY SABRINA LOPES GOMES, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JULIANA MAINENTI LEAL LOPES, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ELYABE MONTEIRO DE MATOS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ELISA GUIMARÃES CABRAL, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ANA LUISA SOUSA AZEVEDO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOSÉ MARCELLO SALABERT DE CAMPOS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LUIZ MENINI NETO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LYDERSON FACIO VICCINI, UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
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Título: |
Phenotypic variation seems not to be associated with the genetic profle in Zygopetalum (Orchidaceae): a case study of a high‑elevation rocky complex. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 51, article 582, 2024. |
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Idioma: |
Inglês |
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Conteúdo: |
Background- Hybridization associated with polyploidy studies is rare in the tropics. The genus Zygopetalum (Orchidaceae) was investigated here as a case study of Neotropical plants. In the rocky highlands of the Ibitipoca State Park (ISP), southeast Brazil, individuals with intermediate colors and forms between the species Z. maculatum and Z. triste were commonly identifed. Methods and results -Chromosomal analysis and DNA quantity showed a uniform population. Regardless of the aspects related to the color and shape of foral structures, all individuals showed 2n=96 chromosomes and an average of 14.05 pg of DNA. Irregularities in meiosis associated with chromosome number and C value suggest the occurrence of polyploidy. The genetic distance estimated using ISSR molecular markers revealed the existence of genetic variability not related to morphological clusters. Morphometric measurements of the fower pieces revealed that Z. maculatum shows higher variation than Z. triste although lacking a defned circumscription. Conclusion - The observed variation can be explained by the polyploid and phenotypic plasticity resulting from the interaction of the genotypes with the heterogeneous environments observed in this habitat. |
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Palavras-Chave: |
Perfil genético; Polyploidy · Rocky outcrops. |
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Thesagro: |
Orchidaceae; Poliploidia; Variação Genética. |
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Thesaurus Nal: |
Genetic variation; Phenotypic plasticity; Polyploidy. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02213naa a2200313 a 4500
001 2164075
005 2024-05-03
008 2024 bl uuuu u00u1 u #d
100 1 $aGOMES, S. S. L.
245 $aPhenotypic variation seems not to be associated with the genetic profle in Zygopetalum (Orchidaceae)$ba case study of a high‑elevation rocky complex.$h[electronic resource]
260 $c2024
520 $aBackground- Hybridization associated with polyploidy studies is rare in the tropics. The genus Zygopetalum (Orchidaceae) was investigated here as a case study of Neotropical plants. In the rocky highlands of the Ibitipoca State Park (ISP), southeast Brazil, individuals with intermediate colors and forms between the species Z. maculatum and Z. triste were commonly identifed. Methods and results -Chromosomal analysis and DNA quantity showed a uniform population. Regardless of the aspects related to the color and shape of foral structures, all individuals showed 2n=96 chromosomes and an average of 14.05 pg of DNA. Irregularities in meiosis associated with chromosome number and C value suggest the occurrence of polyploidy. The genetic distance estimated using ISSR molecular markers revealed the existence of genetic variability not related to morphological clusters. Morphometric measurements of the fower pieces revealed that Z. maculatum shows higher variation than Z. triste although lacking a defned circumscription. Conclusion - The observed variation can be explained by the polyploid and phenotypic plasticity resulting from the interaction of the genotypes with the heterogeneous environments observed in this habitat.
650 $aGenetic variation
650 $aPhenotypic plasticity
650 $aPolyploidy
650 $aOrchidaceae
650 $aPoliploidia
650 $aVariação Genética
653 $aPerfil genético
653 $aPolyploidy · Rocky outcrops
700 1 $aLOPES, J. M. L.
700 1 $aMATOS, E. M. de
700 1 $aCABRAL, E. G.
700 1 $aAZEVEDO, A. L. S.
700 1 $aMACHADO, M. A.
700 1 $aCAMPOS, J. M. S. de
700 1 $aMENINI NETO, L.
700 1 $aVICCINI, L. F.
773 $tMolecular Biology Reports$gv. 51, article 582, 2024.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/04/2005 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ROMANOWSKI, V.; ARANA, E. I.; MCCARTHY. C. B.; BIEDMA, M. E.; SCIOCCO-CAP, A.; GOLDBERG, A. V.; GHIRINGHELLI, P. D.; PINEDO, F. J. R.; MOSCARDI, F.; RIBEIRO, B. M. |
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Afiliação: |
CNPSO. |
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Título: |
Advances towards improving the insecticidal properties of AgMNPV. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003. |
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Páginas: |
p. 32-33. |
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Idioma: |
Inglês |
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Conteúdo: |
Anticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansion in order to control simultaneous lepidopteran pests would certainly increase the appeal of AgMNPV to soybean growers. Controlled field experiments will address th applicability of these and other genetically improved AgMNPVs in large scale programs. The traceability of the recombinants will be facilitated by the insertion of reporter genes. MenosAnticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansi... Mostrar Tudo |
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Thesagro: |
Baculovirus; Controle Biológico; Praga de Planta; Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02707nam a2200289 a 4500
001 1467932
005 2020-02-27
008 2003 bl uuuu u00u1 u #d
100 1 $aROMANOWSKI, V.
245 $aAdvances towards improving the insecticidal properties of AgMNPV.$h[electronic resource]
260 $aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington$c2003
300 $ap. 32-33.
520 $aAnticarsia gemmatalis is a key pest of soybean in Brazil, Argentina, and other countries. AgMNPV is today the most widely used baculovirus pesticide, as more two million hectares are treated annually. However, a number of problems prevent the expansion of the use of the virus to the ca. 20 million hectares of soybean cultivated in South America. A major drawnack is the low speed of kill of wt AgMNPV, which becomes extremely importants in areas with lower temperatures (20o.C). In order to address this problem we have recently developed a system for the genetic modification of AgMNPV. To expand the number of alternative genetic modification we introduced two unique sites for the intron-encoded IPpol endonuclease to linearize the viral DNA used in cotransfections, which greatly reduced the background of non recombinant progeny. The insertion of the insect-specific neurotoxin gene isolated from the mite Pyemotes tritici (TxP-1) yielded a rAgMNPV that paralysed the host larvae within two days after treatment. On the other hand, the disruption of the egt gene eliminated the viral enzyme that inactivates ecdysone, thus accelerating the moulting and the cessation of feeding. The egt(-) rAgMNPV killed the larvae 1-2.8 days faster than the wt virus (mean reduction of LT50 across virus concentrations: 2.2 days) and exhibited a higher virulence (LC50 3.9-fold lower than wt). Both rAgMNPVs significantly reduced the damage caused by the pest. Additionally, strategies of host range expansion in order to control simultaneous lepidopteran pests would certainly increase the appeal of AgMNPV to soybean growers. Controlled field experiments will address th applicability of these and other genetically improved AgMNPVs in large scale programs. The traceability of the recombinants will be facilitated by the insertion of reporter genes.
650 $aSoybeans
650 $aBaculovirus
650 $aControle Biológico
650 $aPraga de Planta
650 $aSoja
700 1 $aARANA, E. I.
700 1 $aMCCARTHY. C. B.
700 1 $aBIEDMA, M. E.
700 1 $aSCIOCCO-CAP, A.
700 1 $aGOLDBERG, A. V.
700 1 $aGHIRINGHELLI, P. D.
700 1 $aPINEDO, F. J. R.
700 1 $aMOSCARDI, F.
700 1 $aRIBEIRO, B. M.
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