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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
02/03/2021 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
Thesagro: |
Staphylococcus Aureus. |
Thesaurus Nal: |
Vaccines. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02811naa a2200349 a 4500 001 2130440 005 2024-02-06 008 2020 bl uuuu u00u1 u #d 100 1 $aCUNHA, A. F. 245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource] 260 $c2020 520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. 650 $aVaccines 650 $aStaphylococcus Aureus 653 $aImmunoproteomics 653 $aIntramammary infection 653 $aSerological proteome analysis 700 1 $aANDRADE, H. M. 700 1 $aSOUZA, F. N. 700 1 $aFIALHO JUNIOR, L. C. 700 1 $aROSA, D. L. S. O. 700 1 $aSANCHEZ, E. M. R. 700 1 $aGIDLUND, M. 700 1 $aGOTO, H. 700 1 $aBRITO, M. A. V. P. 700 1 $aGUIMARÃES, A. S. 700 1 $aLAGE, A. P. 700 1 $aREIS, L. C. 700 1 $aLIBERA, A. M. M. P. D. 700 1 $aHEINEMANN, M. B. 700 1 $aCERQUEIRA, M. M. O. P 773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
08/01/2010 |
Data da última atualização: |
14/06/2013 |
Tipo da produção científica: |
Monitoramento/Zoneamento |
Autoria: |
CHAGAS, C. da S.; BHERING, S. B.; CARVALHO JUNIOR, W. de; AMARAL, F. C. S. do; MONTENEGRO, T. H. M.; ÁGLIO, M. L. D. |
Afiliação: |
CESAR DA SILVA CHAGAS, CNPS; SILVIO BARGE BHERING, CNPS; WALDIR DE CARVALHO JUNIOR, CNPS; FERNANDO CEZAR SARAIVA DO AMARAL, CNPS; TADEU HENRIQUES MONTENEGRO, CODEVASF; MÁRIO LUIZ DIAMANTE ÁGLIO, ASSISTENTE DE PESQUISA CNPS. |
Título: |
Reavaliação do levantamento de solos e da irrigabilidade das terras projeto Jequitaí município de Jequitaí, Estado de Minas Gerais. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Rio de janeiro: Embrapa Solos; Brasília: CODEVASF, 2009. |
Idioma: |
Português |
Palavras-Chave: |
Argissolos vermelhos e amarelos; Latossolos vermelhos-amarelos. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 00651nam a2200181 a 4500 001 1579965 005 2013-06-14 008 2009 bl uuuu t 00u1 u #d 100 1 $aCHAGAS, C. da S. 245 $aReavaliação do levantamento de solos e da irrigabilidade das terras projeto Jequitaí município de Jequitaí, Estado de Minas Gerais. 260 $aRio de janeiro: Embrapa Solos; Brasília: CODEVASF$c2009 653 $aArgissolos vermelhos e amarelos 653 $aLatossolos vermelhos-amarelos 700 1 $aBHERING, S. B. 700 1 $aCARVALHO JUNIOR, W. de 700 1 $aAMARAL, F. C. S. do 700 1 $aMONTENEGRO, T. H. M. 700 1 $aÁGLIO, M. L. D.
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