Data/hora: 10/06/2024 13:38:30

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 15/09/2015
Data da última atualização: | 06/09/2021
Autoria: | BEZERRA JUNIOR, R. Q.; ELOY, A. M. X.; FURTADO, J. R.; PINHEIRO, R. R.; ANDRIOLI, A.
Afiliação: | Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; ANGELA MARIA XAVIER ELOY, CNPC; JOAO RICARDO FURTADO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; ALICE ANDRIOLI, CNPC.
Título: | Matrix metalloproteinases (MMPs) in buck blood chronically infected by arthritis encephalitis virus in Northeast of Brazil.
Ano de publicação: | 2015
Fonte/Imprenta: | In: INTERNATIONAL CONGRESS ON ANALYTICAL PROTEOMICS, 4., Caparica. Book of abstracts; proceedings. Caparica: Universidade Nova de Lisboa, 2015. Abstract O8A. p. 83.
Idioma: | Inglês
Conteúdo: | Abstract: The objective of this work was to investigate the activity of the MMPs (MMP-2 e MMP-9) in blood serum of chronically infected bucks by CAEV. The experiment was constituted by two groups (n = 5 each group). The first one was composed by five naturally infected bucks (4-5 years) and the second group constituted of five negative bucks (4-5 years) for CAE. Serology was performed using the Western Blotting (WB) and confirmed by Polymerase Chain Reaction (PCR). Blood samples were collected by puncturing the jugular vein from animals and evaluated by zymography (SDS-PAGE) using gelatin as substratum. The gel densitometry analysis showed higher activity (P<0.05) of the pro-MMP-2 and MMP-2 in seropositive group (Fig. 1). The higher activity of the MMPs (MMP-2 and MMP-9) in infected group to CAE suggests a role of these proteases in the chronic inflammatory process in goats, once the key role of MMPs molecules in inflammation are involved in pathophysiological remodeling of the vascular wall, even in animals without apparent disease.
Palavras-Chave: | CAEV; Caprine arthritis encephalit virus; Genômica; Proteases.
Thesagro: | Caprino; Protease.
Thesaurus Nal: | Genomics; Goats.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (29111-1) - UPC - RA - DD -  -  -  - DIS