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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
09/03/2010 |
Data da última atualização: |
26/04/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FARIA, J. C.; VALDISSER, P. A. M. R.; ARAGÃO, F. J. L. |
Afiliação: |
JOSIAS CORREA DE FARIA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
Título: |
Epidemiologia de mosaico dourado em feijoeiro transgênico com resistência derivada do patógeno. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 34, p. S124, ago. 2009. Suplemento, ref. 449. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, ago. 2009. |
ISSN: |
1982-5676 |
Idioma: |
Português |
Thesagro: |
Epidemiologia; Feijão; Mosaico dourado; Planta transgênica. |
Thesaurus Nal: |
Bean golden mosaic virus. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/660328/1/cbf-2009-pS124.pdf
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Marc: |
LEADER 00765nam a2200193 a 4500 001 1660328 005 2023-04-26 008 2009 bl uuuu u00u1 u #d 022 $a1982-5676 100 1 $aFARIA, J. C. 245 $aEpidemiologia de mosaico dourado em feijoeiro transgênico com resistência derivada do patógeno.$h[electronic resource] 260 $aTropical Plant Pathology, Brasília, DF, v. 34, p. S124, ago. 2009. Suplemento, ref. 449. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, ago. 2009.$c2009 650 $aBean golden mosaic virus 650 $aEpidemiologia 650 $aFeijão 650 $aMosaico dourado 650 $aPlanta transgênica 700 1 $aVALDISSER, P. A. M. R. 700 1 $aARAGÃO, F. J. L.
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Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
20/01/2015 |
Data da última atualização: |
08/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CRATIVOL, C.; REGULSKI, M.; BERTALAN, M.; MCCOMBIE, W. R.; SILVA, F. R. da; ZERLOTINI NETO, A.; VICENTINI, R.; FARINELLI, L.; HEMERLY, A. S.; MARTIENSSEN, R. A.; FERREIRA, P. C. G. |
Afiliação: |
CLÍCIA GRATIVOL, UFRJ; MICHAEL REGULSKI, Cold Spring Harbor Laboratory; MARCELO BERTALAN, Institute of Biological Psychiatry, Mental Health Center; W. RICHARD MCCOMBIE, Cold Spring Harbor Laboratory; FELIPE RODRIGUES DA SILVA, CNPTIA; ADHEMAR ZERLOTINI NETO, CNPTIA; RENATO VICENTINI, CBMEG/Unicamp; LAURENT FARINELLI, Fasteris SA; ADRIANA SILVA HEMERLY, UFRJ; ROBERT A. MARTIENSSEN, Cold Spring Harbor Laboratory; PAULO CAVALCANTI GOMES FERREIRA, UFRJ. |
Título: |
Sugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus Saccharum. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
The Plant Journal, Oxford, v. 79, n. 1, p. 162-172, 2014. |
DOI: |
10.1111/tpj.12539 |
Idioma: |
Inglês |
Conteúdo: |
Many economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single-nucleotide polymorphisms (SNPs) in the wild sugarcane species, S. spontaneum and S. officinarum. A large number of microRNA genes was also identified in the MF scaffolds. The information achieved by the MF dataset provides a valuable tool for genomic research in the genus Saccharum and for improvement of sugarcane as a biofuel crop. MenosMany economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single... Mostrar Tudo |
Palavras-Chave: |
Cana-de-açúcar; De novo assembly; Gene-rich regions. |
Thesagro: |
Metilação. |
Thesaurus NAL: |
Methylation; Saccharum; Sugarcane. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02815naa a2200337 a 4500 001 2006128 005 2020-01-08 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1111/tpj.12539$2DOI 100 1 $aCRATIVOL, C. 245 $aSugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus Saccharum.$h[electronic resource] 260 $c2014 520 $aMany economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single-nucleotide polymorphisms (SNPs) in the wild sugarcane species, S. spontaneum and S. officinarum. A large number of microRNA genes was also identified in the MF scaffolds. The information achieved by the MF dataset provides a valuable tool for genomic research in the genus Saccharum and for improvement of sugarcane as a biofuel crop. 650 $aMethylation 650 $aSaccharum 650 $aSugarcane 650 $aMetilação 653 $aCana-de-açúcar 653 $aDe novo assembly 653 $aGene-rich regions 700 1 $aREGULSKI, M. 700 1 $aBERTALAN, M. 700 1 $aMCCOMBIE, W. R. 700 1 $aSILVA, F. R. da 700 1 $aZERLOTINI NETO, A. 700 1 $aVICENTINI, R. 700 1 $aFARINELLI, L. 700 1 $aHEMERLY, A. S. 700 1 $aMARTIENSSEN, R. A. 700 1 $aFERREIRA, P. C. G. 773 $tThe Plant Journal, Oxford$gv. 79, n. 1, p. 162-172, 2014.
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