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| 4. |  | SILVA, E. M. da; DINIZ, R. P.; SOUSA, M. B. e; BRAATZ, L. R.; OLIVEIRA, E. J. de. Geração de clones superiores de mandioca em progênies F1, S1 e S2 In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 13., 2019. Foco e valor : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura,2019. 119 p. il.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | MACHADO, L. A.; CRUZ, B. P. B.; LEITE, L. G.; BATISTA FILHO, A.; SILVA, E. M. da. Controle biologico da broca dos citros Campinas, SP: Sociedade Entomologica do Brasil, 1993. p.73-82 In: CICLO DE PALESTRAS SOBRE CONTROLE BIOLOGICO DE PRAGAS, 3, 1993, Campinas, SP. Anais...| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. |
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Título: |
Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 321. |
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Série: |
(Embrapa Soja. Documentos, 228).
|
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
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Conteúdo: |
It is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression. MenosIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this m... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03691naa a2200229 a 4500
001 1466852
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aFUGANTI, R.
245 $aMicrosatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica.
260 $c2004
300 $ap. 321.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression.
700 1 $aBENEVENTI, M. A.
700 1 $aSILVA, J. F. V.
700 1 $aARIAS, C. A. A.
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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