| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
05/12/2016 |
|
Data da última atualização: |
05/12/2016 |
|
Autoria: |
FLÓREZ, L. M. M.; BALLESTERO, H. F.; DUZANSKI, A. P.; BERSANO, P. R. O.; LIMA, J. F.; CRUZ, F. L.; MOTA, L. S.; ROCHA, N. S. |
|
Afiliação: |
LUIS M. M. FLÓREZ, UNESP/Universidade de Caldas; HALINE F. BALLESTERO, UNESP; ANDERSON P. DUZANSKI, UNESP; PAULO R. O. BERSANO, UECE; JOÃO F. LIMA, CEVAP/UNESP; FERNANDA L. CRUZ, UNESP; LÍGIA S. MOTA, UNESP; NOEME S. ROCHA, UNESP. |
|
Título: |
Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 844-850, set. 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. |
|
Palavras-Chave: |
Alfa 1 antitripsina; Alpha 1 antitrypsin; Canine transmissible venereal tumo; Isozima; Tumor venéreo transmissível canino; Vimentina. |
|
Thesagro: |
Citogenética. |
|
Thesaurus Nal: |
Cytogenetics; Lysozyme; Vimentin. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151255/1/Immunocytochemical-characterization.pdf
|
|
Marc: |
LEADER 02414naa a2200325 a 4500
001 2057977
005 2016-12-05
008 2016 bl uuuu u00u1 u #d
100 1 $aFLÓREZ, L. M. M.
245 $aImmunocytochemical characterization of primary cell culture in canine transmissible venereal tumor.
260 $c2016
520 $aImmunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor.
650 $aCytogenetics
650 $aLysozyme
650 $aVimentin
650 $aCitogenética
653 $aAlfa 1 antitripsina
653 $aAlpha 1 antitrypsin
653 $aCanine transmissible venereal tumo
653 $aIsozima
653 $aTumor venéreo transmissível canino
653 $aVimentina
700 1 $aBALLESTERO, H. F.
700 1 $aDUZANSKI, A. P.
700 1 $aBERSANO, P. R. O.
700 1 $aLIMA, J. F.
700 1 $aCRUZ, F. L.
700 1 $aMOTA, L. S.
700 1 $aROCHA, N. S.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 39, n. 9, p. 844-850, set. 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Soja; Embrapa Unidades Centrais. |
|
Data corrente: |
24/03/2011 |
|
Data da última atualização: |
14/10/2011 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C. |
|
Afiliação: |
RENATA STOLF-MOREIRA, UNESP Jaboticabal; ELIANA GERTRUDES DE MACEDO LEMOS, UNESP Jaboticabal; RICARDO VILELA ABDELNOOR, CNPSO; MAGDA APARECIDA BENEVENTI, UFRGS; AMANDA ALVES PAIVA ROLLA, UEL; SELMA DOS SANTOS PEREIRA, UEL; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO. |
|
Título: |
Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 1, p. 58-65, jan. 2011. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the GmB-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis. |
|
Palavras-Chave: |
Déficit hídrico; Estabilidade de expressão; Expressão gênica; Expression stability; RT-qPCR. |
|
Thesagro: |
Biotecnologia; Deficiência hídrica; Glycine Max; Soja. |
|
Thesaurus NAL: |
Agricultural biotechnology; Gene expression; Plant-water relations; Soybeans. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31023/1/46n01a08.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168638/1/Identification-of-reference-genes-for-expression.pdf
|
|
Marc: |
LEADER 02275naa a2200373 a 4500
001 1882641
005 2011-10-14
008 2011 bl uuuu u00u1 u #d
100 1 $aSTOLF-MOREIRA, R.
245 $aIdentification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean.
260 $c2011
520 $aThe objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the GmB-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.
650 $aAgricultural biotechnology
650 $aGene expression
650 $aPlant-water relations
650 $aSoybeans
650 $aBiotecnologia
650 $aDeficiência hídrica
650 $aGlycine Max
650 $aSoja
653 $aDéficit hídrico
653 $aEstabilidade de expressão
653 $aExpressão gênica
653 $aExpression stability
653 $aRT-qPCR
700 1 $aLEMOS, E. G. de M.
700 1 $aABDELNOOR, R. V.
700 1 $aBENEVENTI, M. A.
700 1 $aROLLA, A. A. P.
700 1 $aPEREIRA, S. dos S.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aNEPOMUCENO, A. L.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 46, n. 1, p. 58-65, jan. 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
