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Registros recuperados : 197 | |
66. | | MINUZZO, J.; TEMPASS, C. R.; MICHELON, M. F.; RIFATO, L.; RUFATO, A. de R. Manejo da carga de frutos de macieira da cultivar Gala através da poda de precisão. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 48. (Embrapa Uva e Vinho. Documentos, 99) Biblioteca(s): Embrapa Uva e Vinho. |
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70. | | LIMA, C. S. M.; GALARÇA, S. P.; BETEMPS, D. L.; RUFATO, A. de R.; RUFATO, L. Avaliação física, química e fitoquímica de frutos de Physalis, ao longo do período de colheita. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 4, p. 1004-1012, dez. 2012. Biblioteca(s): Embrapa Uva e Vinho. |
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71. | | VILANOVA, D. S.; LIMA, C. S. M.; GAUTERIO, G.; RUFATO, A. de R.; RUFATO, L. Capacidade de germinação de sementes de Physalis peruviana L. em função do período de armazenamento. In: SIMPÓSIO NACIONAL DO MORANGO, 5.; ENCONTRO SOBRE PEQUENAS FRUTAS E FRUTAS NATIVAS DO MERCOSUL, 4., 2010, Pelotas. Palestras e resumos... Pelotas: Embrapa Clima Temperado, 2010. p. 162-163. Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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72. | | MICHELON, M. F.; CARMINATTI, J. F.; RUFATO, L.; RUFATO, A. de R.; FRANCESCATTO, P. Associação de ácido abcísico e benziladenina no raleio químico de macieiras "Royal Gala". In.:ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 14., 2015, Fraiburgo, SC. Anais...(v.2 - Resumos). Caçador: Epagri, v. 2 (trabalhos), 2015. p. 47. Biblioteca(s): Embrapa Uva e Vinho. |
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74. | | GALARÇA, S. P.; LIMA, C. S. M.; SILVEIRA, G. da; RUFATO, A. de R. Correlação de pearson e análise de trilha identificando variáveis para caracterizar porta-enxerto de Pyrus communis L. Ciência e Agrotecnologia, Lavras, v. 34, n. 4, p. 860-869, jul./ago., 2010. Biblioteca(s): Embrapa Uva e Vinho. |
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77. | | RUFATO, L.; KRETZSCHMAR, A. A.; SCHLEMPER, C.; BRIGHENTI, A. F.; SÄHELIN, V.; RUFATO, A. de R. Cluster thinning effects on grape composition of Sangiovese in high altitude regions of Santa Catarina State - Brazil. In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and Horticulture for people: abstracts. Lisboa: ISHS, 2010. p. 722. Resumo S16.243. Biblioteca(s): Embrapa Uva e Vinho. |
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Registros recuperados : 197 | |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
Data corrente: |
22/08/2022 |
Data da última atualização: |
22/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 5 |
Autoria: |
NICIURA, S. C. M.; BENAVIDES, M. V.; OKINO, C. H.; IBELLI, A. M. G.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; ALESSANDRO PELEGRINE MINHO, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Pathogens, v. 11, n. 8, aug. 2022, 939. |
Páginas: |
12 p. |
DOI: |
https://doi.org/10.3390/pathogens11080939 |
Idioma: |
Inglês |
Conteúdo: |
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. MenosAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for... Mostrar Tudo |
Palavras-Chave: |
Gastrointestinal nematode control; GWAS; Molecular markers; Ovine; Parasite resistance. |
Thesagro: |
Marcador Molecular; Ovino; Parasito. |
Thesaurus NAL: |
Genotyping. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145606/1/GenomeWideAssociation.pdf
|
Marc: |
LEADER 02449naa a2200325 a 4500 001 2145606 005 2022-08-22 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/pathogens11080939$2DOI 100 1 $aNICIURA, S. C. M. 245 $aGenome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.$h[electronic resource] 260 $c2022 300 $a12 p. 520 $aAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. 650 $aGenotyping 650 $aMarcador Molecular 650 $aOvino 650 $aParasito 653 $aGastrointestinal nematode control 653 $aGWAS 653 $aMolecular markers 653 $aOvine 653 $aParasite resistance 700 1 $aBENAVIDES, M. V. 700 1 $aOKINO, C. H. 700 1 $aIBELLI, A. M. G. 700 1 $aMINHO, A. P. 700 1 $aESTEVES, S. N. 700 1 $aCHAGAS, A. C. de S. 773 $tPathogens$gv. 11, n. 8, aug. 2022, 939.
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Embrapa Pecuária Sudeste (CPPSE) |
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