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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
18/12/2018 |
Data da última atualização: |
18/12/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PINHEIRO, A. F.; ROLOFF, B. C.; MOREIRA, A. da S.; BERNE, M. E. A.; ANDREOTTI, R.; LEITE, F. P. L. |
Afiliação: |
Amanda Fernandes Pinheiro, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; Bárbara Couto Roloff, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Angelita da Silveira Moreira, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Maria Elisabeth Aires Berne, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; RENATO ANDREOTTI E SILVA, CNPGC; Fábio Pereira Leivas Leite, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas – UFPel. |
Título: |
Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Vaccine, v. 36, n. 9, p. 1154-1159, February 2018 |
Idioma: |
Inglês |
Conteúdo: |
The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis. MenosThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In concl... Mostrar Tudo |
Thesagro: |
Neospora Caninum. |
Thesaurus Nal: |
Adjuvants; Cytokines; Proteins; Vaccines. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188808/1/Identification-of-suitable-adjuvant-for-vaccine.pdf
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Marc: |
LEADER 02555naa a2200241 a 4500 001 2101958 005 2018-12-18 008 2018 bl uuuu u00u1 u #d 100 1 $aPINHEIRO, A. F. 245 $aIdentification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2.$h[electronic resource] 260 $c2018 520 $aThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis. 650 $aAdjuvants 650 $aCytokines 650 $aProteins 650 $aVaccines 650 $aNeospora Caninum 700 1 $aROLOFF, B. C. 700 1 $aMOREIRA, A. da S. 700 1 $aBERNE, M. E. A. 700 1 $aANDREOTTI, R. 700 1 $aLEITE, F. P. L. 773 $tVaccine$gv. 36, n. 9, p. 1154-1159, February 2018
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Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
28/09/2012 |
Data da última atualização: |
28/09/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ROCHA, M. do S.; BELTRAO, N. E. de M. |
Afiliação: |
Maria do Socorro Rocha, Bolsista PNPD na área de Fisiologia vegetal , doutora em Agronomia UFPB; NAPOLEAO ESBERARD DE MACEDO BELTRAO, CNPA. |
Título: |
Crescimento de mudas de pinhão manso (Jatropha curcas L.) sob estresse salino diferestes doses de sais. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. |
Páginas: |
p. 137 |
Idioma: |
Português |
Palavras-Chave: |
JATROPHA CURCAS L; PINHÃO MANSO. |
Thesagro: |
Área Foliar; Condutividade Eletrica; Fisiologia; Germinação; Irrigação. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67148/1/FIS-051-P.035.pdf
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Marc: |
LEADER 00791nam a2200205 a 4500 001 1935069 005 2012-09-28 008 2012 bl uuuu u00u1 u #d 100 1 $aROCHA, M. do S. 245 $aCrescimento de mudas de pinhão manso (Jatropha curcas L.) sob estresse salino diferestes doses de sais. 260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão$c2012 300 $ap. 137 650 $aÁrea Foliar 650 $aCondutividade Eletrica 650 $aFisiologia 650 $aGerminação 650 $aIrrigação 653 $aJATROPHA CURCAS L 653 $aPINHÃO MANSO 700 1 $aBELTRAO, N. E. de M.
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