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| 82. |  | COSTA, A. S.; PRATES, H. S.; MULLER, G. W. Possivel identidade entre o chicote da laranjeira piralima, o envassouramento da laranjeira pera e a brotacao multipla dos citros Campinas: Sociedade Brasileira de Fruticultura, 1988. v.1 p.417-422 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 9., Campinas, 1987. Anais...Campinas, Sociedade Brasileira de Fruticultura, 1988.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 90. | | MUNDIM, A. V.; COSTA, A. S.; MUNDIM, S. A. P.; GUIMARÃES, E. C.; ESPÍNDOLA, F. S. Influência da ordem e estádios da lactação no perfil bioquímico sangüíneo de cabras da raça Saanen. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 59, n. 2, p. 306-312, abr. 2007.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 98. | | ARAGÃO, W. M.; COSTA, A. S. da; SANTOS, H. C. A. C.; PEDROSO, G. T. Florescimento, produção e composição morfológica de frutos de cultivares de coqueiro. Agrotrópica, Ilhéus, v.14, n.3, p.151-158, 2002.| Biblioteca(s): Embrapa Semiárido. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/02/2022 |
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Data da última atualização: |
07/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
TAIRA, A. R.; BATISTA, R. I. T. P.; SANTOS, J. D. R.; PINTO, P. H. N.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; SOUZA-FABJAN, J. M. G.; UNGERFELD, R.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense - Niterói, RJ, Brazil; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense - Niterói, RJ, Brazil; JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense - Niterói, RJ, Brazil; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; MARIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense - Niterói, RJ, Brazil; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; VIVIANE LOPES BRAIR, Universidade Federal Fluminense - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense - Niterói, RJ, Brazil. |
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Título: |
Progestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 238, 106938, Mar. 2022. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2022.106938 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene. MenosAbstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the ... Mostrar Tudo |
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Palavras-Chave: |
Day 0 protocol; Embryo production; Multiple ovulations; Progestins. |
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Thesaurus NAL: |
Animal reproduction; Estrous cycle; Ewes; Follicular development; Gene expression; Gene expression regulation; Medroxyprogesterone; Ovarian development; Progesterone; Small ruminants; Superovulation; Synthetic progestogens. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02951naa a2200445 a 4500
001 2139763
005 2022-02-07
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2022.106938$2DOI
100 1 $aTAIRA, A. R.
245 $aProgestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep.$h[electronic resource]
260 $c2022
520 $aAbstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene.
650 $aAnimal reproduction
650 $aEstrous cycle
650 $aEwes
650 $aFollicular development
650 $aGene expression
650 $aGene expression regulation
650 $aMedroxyprogesterone
650 $aOvarian development
650 $aProgesterone
650 $aSmall ruminants
650 $aSuperovulation
650 $aSynthetic progestogens
653 $aDay 0 protocol
653 $aEmbryo production
653 $aMultiple ovulations
653 $aProgestins
700 1 $aBATISTA, R. I. T. P.
700 1 $aSANTOS, J. D. R.
700 1 $aPINTO, P. H. N.
700 1 $aBALARO, M. F. A.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aBRAIR, V. L.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aUNGERFELD, R.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tAnimal Reproduction Science$gv. 238, 106938, Mar. 2022.
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