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| Registros recuperados : 102 | |
| 6. |  | BATISTA, R. I. T. P.; DIAS, J. H.; GONÇALVES, J. D.; VERGANI, G. B.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. The presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile. Theriogenology, v. 218, p. 208-213, Feb. 2024.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. Goat incubator: the doe as a life incubator of bovine oocytes - first step. Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | CAMPOS JUNIOR, P. H. A.; ASSUNÇÃO, C. M.; CARVALHO, B. C. de; BATISTA, R. I. T. P.; GARCIA, R. M. G.; VIANA, J. H. M. Follicular populations, recruitment and atresia in the ovaries of different strains of mice. Reproductive Biology, v. 12, n. 1. p. 41-55, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; VIANA, J. H. M. Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 70, n. 5, p. 1489-1496, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | VIANA, S. W.; BOITÉ, M. C.; PEREIRA, M. M.; BATISTA, R. I. T. P.; MOTA, G. B.; VIANA, J. H. M.; CAMARGO, L. S. A.; MACHADO, M. A. Identificação de transcritos da família aquaporina em embriões bovinos (resultados parciais). In: SEMANA DE BIOLOGIA, 30., 2007, Juiz de Fora. O mundo se tornou pequeno para você mas você não é grande para o mundo: anais. Juiz de Fora: DACBIO/UFJF, 2007. p. 58-64.| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | WOHLRES, S.; PINTO, I. S. B.; BATISTA, R. I. T. P.; FAZA, A. P.; PAIVA, F. P.; MAFFILI, V. V.; CAMARGO, L. S. A.; VIANA, J. H. M. Are superovulation protocols realy efficient for embryo production in inbred mice lineages? Acta Scientiae Veterinariae, v. 34, s584, 2006. Supl. 1. Edição dos resumos da 20 Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | GRÁZIA, J. G. V. de; BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; GARCIA, R. M. G.; IGUMA, L. T.; VIANA, J. H. M. Avaliação do acúmulo de lipídeos em oócitos de vacas Gir (Bos indicus) e Holandesa (Bos taurus). In: MOSTRA CIENTÍFICA, 16.; SEMANA DE BIOLOGIA DA UFJF, 33., 2010, Juiz de Fora. Anais... Juiz de Fora: UFJF/Dacbio, 2010. p. 43-46.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; MELO, L. M.; OLIVEIRA, M. A.; CHAVES, M. S.; FONSECA, J. F. da; FREITAS, V. J. F. Transcervical versus laparotomy embryo recovery: what strategy is best for embryo bank formation in the Caninde goat conservation program?. Biopreservation and Biobanking, v. 20, n. 2, p. 204-207, Sept. 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | FONSECA, J. F. da; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BATISTA, R. I. T. P.; GARCIA, A. R.; BARTLEWSKIE, P. M.; SOUZA-FABJAN, J. M. G. Non-surgical embryo transfer in goats and sheep: the Brazilian experience. Reproduction Fertility and Development, v. 31, n. 1 p. 17-26, Dec. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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| 17. | | SOUZA-FABJANA, J. M. G.; BATISTA, R. I. T. P.; CORREIA, L. F. L; PARAMIO, M. T.; FONSECA, J. F. da; FREITAS, V. J. F.; MERMILLOD, P. In vitro production of small ruminant embryos: latest improvements and further research. Reproduction, Fertility and Development, v. 33, n. 2, p. 31-54, Jan. 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | PINTO, P. H. N.; BALARO, M. F. A.; ARASHIRO, E. K. N.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; BRAGANÇA, G. M.; FONSECA, J. F. da; BRANDÃO, F. Z. Produção in vivo de embriões ovinos. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 208-216, jan./mar. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | FIGUEIRA, L. M.; ALVES, N. G.; BATISTA, R. I. T. P.; BRAIR, V. L.; LIMA, R. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da; SOUZA-FABJAN, J. M. G. Pregnancy rate after fixed-time transfer of cryopreserved embryos collected by non-surgical route in Lacaune sheep. Reproduction in Domestic Animals, v. 54, n. 11, p. 1493-496, Nov. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | BRANDÃO, F. Z.; PINTO, P. H. N.; BRAGANÇA, G. M.; RIBEIRO, L. dos S.; BALARO, M. F. A.; BATISTA, R. I. T. P.; ARASHIRO, E. K. N.; FONSECA, J. F. da. Hormônio anti-mulleriano(AMH) e produção in vivo de embriões em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 475, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 102 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/01/2020 |
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Data da última atualização: |
23/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. |
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Afiliação: |
VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINA CERQUEIRA SARMENTO OLIVARES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUANA RANGEL CÔRTES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. |
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DOI: |
10.22456/1679-9216.98317 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. MenosBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, ... Mostrar Tudo |
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Palavras-Chave: |
Billygoats; CTC. |
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Thesagro: |
Bode; Bovino; Caprino; Carneiro; Espermatozóide; Heparina; Ovino; Reprodução Animal; Sêmen. |
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Thesaurus NAL: |
Animal reproduction; Assisted reproductive technologies; Bulls; Cattle; Goats; Heparin; Rams; Sheep; Sperm capacitation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209450/1/cnpc-2019-Mini-Percoll.pdf
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Marc: |
LEADER 04821naa a2200469 a 4500
001 2119218
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.22456/1679-9216.98317$2DOI
100 1 $aALFRADIQUE, V. A. P.
245 $aMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.$h[electronic resource]
260 $c2019
520 $aBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique.
650 $aAnimal reproduction
650 $aAssisted reproductive technologies
650 $aBulls
650 $aCattle
650 $aGoats
650 $aHeparin
650 $aRams
650 $aSheep
650 $aSperm capacitation
650 $aBode
650 $aBovino
650 $aCaprino
650 $aCarneiro
650 $aEspermatozóide
650 $aHeparina
650 $aOvino
650 $aReprodução Animal
650 $aSêmen
653 $aBillygoats
653 $aCTC
700 1 $aOLIVARES, C. C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aBALARO, M. F. A.
700 1 $aSARAIVA, H. F. R. de A.
700 1 $aCÔRTES, L. R.
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1707, p. 1-8, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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