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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/02/2018 |
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Data da última atualização: |
06/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. S.; SEYFRIED, M.; CAMPESTRINI, L. H.; BAGGIO, S. F. Z.; MINHO, A. P.; MOLENTO, M. B.; MAURER, J. B. B. |
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Afiliação: |
Andressa Soldera Silva, UFPR; Melina Seyfried, UFPR; Luciano Henrique Campestrini, UFPR; Selma Faria Zawadzki Baggio, UFPR; ALESSANDRO PELEGRINE MINHO, CPPSE; Marcelo Beltrão Molento, UFPR; Juliana Bello Baron Maurer, UFPR. |
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Título: |
Assessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 251, p. 34-43, feb. 2018. |
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DOI: |
10.1016/j.vetpar.2017.12.019 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications. MenosThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7&#... Mostrar Tudo |
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Palavras-Chave: |
Avocado. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus Nal: |
epicatechin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03149naa a2200241 a 4500
001 2087019
005 2018-12-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2017.12.019$2DOI
100 1 $aSILVA, A. S.
245 $aAssessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts.$h[electronic resource]
260 $c2018
520 $aThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications.
650 $aepicatechin
650 $aHaemonchus Contortus
653 $aAvocado
700 1 $aSEYFRIED, M.
700 1 $aCAMPESTRINI, L. H.
700 1 $aBAGGIO, S. F. Z.
700 1 $aMINHO, A. P.
700 1 $aMOLENTO, M. B.
700 1 $aMAURER, J. B. B.
773 $tVeterinary Parasitology$gv. 251, p. 34-43, feb. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Cutter |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
29/11/1993 |
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Data da última atualização: |
20/07/2020 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
BATISTA, H. A. M.; ALBUQUERQUE, M. de; CAMARAO, A. P.; LOURENÇO JUNIOR, J. de B. |
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Afiliação: |
HERIBERTO ANTONIO MARQUES BATISTA, CPATU; MILTON DE ALBUQUERQUE, CPATU; ARI PINHEIRO CAMARAO, CPATU; JOSE DE BRITO LOURENCO JUNIOR, CPATU. |
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Título: |
Suplementação alimentar com resíduos da agroindústria em bubalinos na fase de crescimento. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1983. |
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Páginas: |
2 p. |
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Série: |
(EMBRAPA-CPATU. Comunicado técnico, 44). |
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Idioma: |
Português |
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Conteúdo: |
Objetivando determinar o nível ideal para aproveitamento do farelo de rama de mandioca na suplementação alimentar de bubalinos em crescimento, foi conduzido no Laboratório de Nutrição Animal do CPATU-EMBRAPA um experimento, utilizando trinta e dois bezerros desmamados, com idade média de dez meses, os quais foram reunidos em oito grupos de quatro animais formados por duas fêmeas e dois machos, em delineamento jinteiramente casualizado. |
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Palavras-Chave: |
Agroindustria; Agroindustrial complexes; Bubalino; Bubalinos; Bufalos; Buffalo; Feed; Residue; Residuos; Restolhos; Restos; Suplementação alimentar; Supplements. |
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Thesagro: |
Alimentação; Alimentação Suplementar; Bubalus Bubalis; Búfalo; Crescimento; Ganho de Peso; Nutrição; Resíduo; Resíduo Industrial; Suplemento Alimentar. |
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Thesaurus NAL: |
nutrition; weight gain. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59257/1/CPATU-ComTec44.pdf
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Marc: |
LEADER 01691nam a2200469 a 4500
001 1382985
005 2020-07-20
008 1983 bl uuuu u0uu1 u #d
100 1 $aBATISTA, H. A. M.
245 $aSuplementação alimentar com resíduos da agroindústria em bubalinos na fase de crescimento.
260 $aBelém, PA: EMBRAPA-CPATU$c1983
300 $a2 p.
490 $a(EMBRAPA-CPATU. Comunicado técnico, 44).
520 $aObjetivando determinar o nível ideal para aproveitamento do farelo de rama de mandioca na suplementação alimentar de bubalinos em crescimento, foi conduzido no Laboratório de Nutrição Animal do CPATU-EMBRAPA um experimento, utilizando trinta e dois bezerros desmamados, com idade média de dez meses, os quais foram reunidos em oito grupos de quatro animais formados por duas fêmeas e dois machos, em delineamento jinteiramente casualizado.
650 $anutrition
650 $aweight gain
650 $aAlimentação
650 $aAlimentação Suplementar
650 $aBubalus Bubalis
650 $aBúfalo
650 $aCrescimento
650 $aGanho de Peso
650 $aNutrição
650 $aResíduo
650 $aResíduo Industrial
650 $aSuplemento Alimentar
653 $aAgroindustria
653 $aAgroindustrial complexes
653 $aBubalino
653 $aBubalinos
653 $aBufalos
653 $aBuffalo
653 $aFeed
653 $aResidue
653 $aResiduos
653 $aRestolhos
653 $aRestos
653 $aSuplementação alimentar
653 $aSupplements
700 1 $aALBUQUERQUE, M. de
700 1 $aCAMARAO, A. P.
700 1 $aLOURENÇO JUNIOR, J. de B.
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Embrapa Amazônia Oriental (CPATU) |
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