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4. | | ARAÚJO, G. L. T.; BASSO, M. F.; MORGANTE, C. V.; SA, M. F. G. de. Overexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 611. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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5. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; BOTTON, M.; AYUB, R. A.; NICKEL, O. RT-PCR para detecção de vírus em vinhedos antigos e em cochonilhas vetoras. Tropical Plant Pathology, v. 35, p. S165, ago. 2010. Suplemento. Resumo (05.012) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá. Biblioteca(s): Embrapa Uva e Vinho. |
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7. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Produção de antissoro policlonal utilizando a proteína capsidial recombinante do Rupestris stem pitting-associated virus. Ciência Rural, Santa Maria, v. 40, n. 11, p. 2385-2388, 2010. Nota técnica. Biblioteca(s): Embrapa Uva e Vinho. |
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8. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Production of polyclonal antiserum using recombinant coat protein of Rupestris stem pitting-associated virus. Tropical Plant Pathology, v. 35, p. S272, ago. 2010. Suplemento. Resumo (11.013) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá. Biblioteca(s): Embrapa Uva e Vinho. |
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10. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas. Ciência Rural, Santa Maria, v. 40, n. 11, p. 2249-2255, 2010. Biblioteca(s): Embrapa Uva e Vinho. |
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11. | | BASSO, M. F.; FAJARDO, T. V. M.; SILVA, J. C. F.; ALFENAS-ZERBINI, P.; ZERBINI, F. M. Association of a novel highly divergent monopartite circular ssDNA virus with chlorotic dwarf and dry branches in apple and pear and potential association with symptoms in grapevine. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 7.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 5., 2013, Hangzhou. Program and abstracts... [S.l.: s.n., 2013]. p. 56. Biblioteca(s): Embrapa Uva e Vinho. |
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12. | | FAJARDO, T. V. M.; BASSO, M. F.; GUERRA, C. C.; SANTOS, H. P. dos; AYUB, R. A.; NICKEL, O. Alterações fisiolágicas e de qualidade da uva produzida em videiras infectadas por vírus. Tropical Plant Pathology, v. 35, p. S191, ago. 2010. Suplemento. Resumo (06.002) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá. Biblioteca(s): Embrapa Uva e Vinho. |
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13. | | BASSO, M. F.; SILVA, J. C. F.; FAJARDO, T. V. M.; FONTES, E. P. B.; ZERBINI, F. M. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine. Virus Research, v. 14, n. 210, p. 27-33, July 2015. Biblioteca(s): Embrapa Uva e Vinho. |
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15. | | BASSO, M. F.; FAJARDO, T. V. M.; SANTOS, H. P. dos; GUERRA, C. C.; AYUB, R. A.; NICKEL, O. Fisiologia foliar e qualidade enológica da uva em videiras infectadas por vírus. Tropical Plant Pathology, Brasília, v. 35, n. 6, p. 351-359, nov./dez. 2010. Biblioteca(s): Embrapa Uva e Vinho. |
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17. | | BASSO, M. F.; FERREIRA, P. C. G.; KOBAYASHI, A. K.; HARMON, F. G.; NEPOMUCENO, A. L.; MOLINARI, H. B. C.; GROSSI-DE-SA, M. F. MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants. Plant Biotechnology Journal, v. 17, p. 1482-1500, 2019. Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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18. | | MARTINS, P. K.; MAFRA, V.; SOUZA, W. R. de; RIBEIRO, A. P.; VINECKY, F.; BASSO, M. F.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis. Scientific Reports, V. 6, 28348, 2016. Biblioteca(s): Embrapa Agroenergia. |
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19. | | BASSO, M. F.; CONTALDI, F.; CELSO, F. lo; BARATTO, C. M.; SA, M. F. G. de; BARONE, G.; FERRANTE, A.; MARTINELLI, F. Identification and expression profile of the SMAX/SMXL family genes in chickpea and lentil provide important players of biotechnological interest involved in plant branching. Planta, v. 259, 2024. 1. Na publicação: Maria Fatima Grossi?de?Sa. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017. Biblioteca(s): Embrapa Agroenergia. |
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Registros recuperados : 49 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
28/04/2020 |
Data da última atualização: |
19/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; BUSANELLO, C.; PINTO, C. E. M.; FREITAS, E. de O.; RIBEIRO, T. P.; ENGLER, J. de A.; OLIVEIRA, A. C. de; MORGANTE, C. V.; ALVES-FERREIRA, M.; GROSSI-DE-SA, M. F. |
Afiliação: |
MARCOS FERNANDO BASSO; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CARLOS BUSANELLO, UFPEL; CLIDIA EDUARDA MOREIRA PINTO, UNB; ELINEA DE OLIVEIRA FREITAS, UNB; JANICE DE ALMEIDA ENGLER, INRA/CNRS/UNS, FRANCE; ANTONIO COSTA DE OLIVEIRA, UFPEL; CAROLINA VIANNA MORGANTE, CPATSA; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Insights obtained using different modules of the cotton uceA1.7 promoter. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Planta, v. 251, n. 2, 2020. |
DOI: |
https://doi.org/10.1007/s00425-020-03348-8 |
Idioma: |
Inglês |
Conteúdo: |
Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops. MenosTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development o... Mostrar Tudo |
Palavras-Chave: |
Cotton constitutive promoter; New biotechnological tools; Transcriptional core promoter; Transgenic crops. |
Thesagro: |
Algodão; Gene Marcador; Genética. |
Thesaurus NAL: |
Gene expression. |
Categoria do assunto: |
-- G Melhoramento Genético |
Marc: |
LEADER 02743naa a2200349 a 4500 001 2123510 005 2020-11-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s00425-020-03348-8$2DOI 100 1 $aBASSO, M. F. 245 $aInsights obtained using different modules of the cotton uceA1.7 promoter.$h[electronic resource] 260 $c2020 520 $aTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops. 650 $aGene expression 650 $aAlgodão 650 $aGene Marcador 650 $aGenética 653 $aCotton constitutive promoter 653 $aNew biotechnological tools 653 $aTranscriptional core promoter 653 $aTransgenic crops 700 1 $aLOURENCO-TESSUTTI, I. T. 700 1 $aBUSANELLO, C. 700 1 $aPINTO, C. E. M. 700 1 $aFREITAS, E. de O. 700 1 $aRIBEIRO, T. P. 700 1 $aENGLER, J. de A. 700 1 $aOLIVEIRA, A. C. de 700 1 $aMORGANTE, C. V. 700 1 $aALVES-FERREIRA, M. 700 1 $aGROSSI-DE-SA, M. F. 773 $tPlanta$gv. 251, n. 2, 2020.
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