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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
27/05/2022 |
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Data da última atualização: |
20/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, M. M. de; NICIURA, S. C. M.; ROCHA, M. I. P.; PAN, Z.; ZHOU, H.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; AFONSO, J.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L.; KOLTES, J. E.; REGITANO, L. C. de A. |
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Afiliação: |
MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCAR; ZHANGYUAN PAN, UNIVERSITY OF CALIFORNIA; HUAIJUN ZHOU, UNIVERSITY OF CALIFORNIA; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; GERSON B. MOURÃO, ESALQ/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; JAMES E. KOLTES, IOWA STATE UNIVERSITY; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Epigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022. |
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DOI: |
https://doi.org/10.1186/s13072-022-00449-4 |
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Idioma: |
Inglês |
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Notas: |
Article number: 15. |
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Conteúdo: |
Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation. MenosAbstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affec... Mostrar Tudo |
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Palavras-Chave: |
EBF3; Epigenoma; Epigenome; Força de cisalhamento; GNAS; Muscle; Músculo de nelore; Nelore; RRBS; Shear force. |
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Thesagro: |
Bos Indicus; Bos Taurus; Metilação; Músculo. |
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Thesaurus Nal: |
Cattle; DNA methylation; Methylation. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144144/1/DNA-MethylationMay.pdf
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Marc: |
LEADER 03129naa a2200505 a 4500
001 2144144
005 2022-06-20
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13072-022-00449-4$2DOI
100 1 $aSOUZA, M. M. de
245 $aDNA methylation may affect beef tenderness through signal transduction in Bos indicus.$h[electronic resource]
260 $c2022
500 $aArticle number: 15.
520 $aAbstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation.
650 $aCattle
650 $aDNA methylation
650 $aMethylation
650 $aBos Indicus
650 $aBos Taurus
650 $aMetilação
650 $aMúsculo
653 $aEBF3
653 $aEpigenoma
653 $aEpigenome
653 $aForça de cisalhamento
653 $aGNAS
653 $aMuscle
653 $aMúsculo de nelore
653 $aNelore
653 $aRRBS
653 $aShear force
700 1 $aNICIURA, S. C. M.
700 1 $aROCHA, M. I. P.
700 1 $aPAN, Z.
700 1 $aZHOU, H.
700 1 $aBRUSCADIN, J. J.
700 1 $aDINIZ, W. J. da S.
700 1 $aAFONSO, J.
700 1 $aOLIVEIRA, P. S. N. de
700 1 $aMOURÃO, G. B.
700 1 $aZERLOTINI NETO, A.
700 1 $aCOUTINHO, L. L.
700 1 $aKOLTES, J. E.
700 1 $aREGITANO, L. C. de A.
773 $tEpigenetics & Chromatin$gv. 15, n. 1, p. 1-16, 2022.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Unidades Centrais. |
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Data corrente: |
13/09/2001 |
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Data da última atualização: |
30/08/2004 |
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Autoria: |
DIAS, L. E.; ALVAREZ, V. H.; JUCKSCH, I.; BARROS, N. F. de; BRIENZA JUNIOR, S. |
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Título: |
Formacao de Mudas de Taxi-Branco (Sclerolobium Paniculatum Voguel) I. Resposta a Calcario e Fosforo. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.26, n.1, p.69-76, jan.1991 |
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Idioma: |
Português |
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Conteúdo: |
Com o intuito de se avaliar o efeito da aplicacao de calcario e de fosforo na formacao de mudas de taxi-branco (Sclerolobium paniculatum Voguel), conduziu-se um ensaio em casa de vegetacao, utilizando-se uma amostra subsuperficial de um LVa como substrato. Os tratamentos foram gerados a partir da matriz experimental Plan Puebla III, tendo como mistura corretiva calcario dolomitico + CaCO3 comercial, e como fonte de P solucoes de sais p.a. Adubacoes complementares de N, K e S foram realizadas ao longo dos 190 dias de cultivo em casa de vegetacao. As equacoes de regressao obtidas para producao de materia seca foliar e de ramos, mostram uma resposta quadratica a edicao de P ao substrato e que nao houve resposta a calagem, sendo que as necessidades de Ca, possivelmente foram satisfeitas a partir da adicao de 0,37 meq/100 cm3 adicionados via gesso, na adubacao basica de S. A absorcao de Ca pela planta foi dependente do nivel de P disponivel no solo. Foram obtidos ainda, níveis criticos de P no solo e na materia seca foliar (26,10 mg/dm3 e 0,12% respectivamente). |
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Palavras-Chave: |
Adubação com fósforo; CaCO3. |
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Thesagro: |
Calagem. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/20781/1/pab11_jan_91.pdf
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Marc: |
LEADER 01681naa a2200205 a 4500
001 1105734
005 2004-08-30
008 1991 bl uuuu u00u1 u #d
100 1 $aDIAS, L. E.
245 $aFormacao de Mudas de Taxi-Branco (Sclerolobium Paniculatum Voguel) I. Resposta a Calcario e Fosforo.
260 $c1991
520 $aCom o intuito de se avaliar o efeito da aplicacao de calcario e de fosforo na formacao de mudas de taxi-branco (Sclerolobium paniculatum Voguel), conduziu-se um ensaio em casa de vegetacao, utilizando-se uma amostra subsuperficial de um LVa como substrato. Os tratamentos foram gerados a partir da matriz experimental Plan Puebla III, tendo como mistura corretiva calcario dolomitico + CaCO3 comercial, e como fonte de P solucoes de sais p.a. Adubacoes complementares de N, K e S foram realizadas ao longo dos 190 dias de cultivo em casa de vegetacao. As equacoes de regressao obtidas para producao de materia seca foliar e de ramos, mostram uma resposta quadratica a edicao de P ao substrato e que nao houve resposta a calagem, sendo que as necessidades de Ca, possivelmente foram satisfeitas a partir da adicao de 0,37 meq/100 cm3 adicionados via gesso, na adubacao basica de S. A absorcao de Ca pela planta foi dependente do nivel de P disponivel no solo. Foram obtidos ainda, níveis criticos de P no solo e na materia seca foliar (26,10 mg/dm3 e 0,12% respectivamente).
650 $aCalagem
653 $aAdubação com fósforo
653 $aCaCO3
700 1 $aALVAREZ, V. H.
700 1 $aJUCKSCH, I.
700 1 $aBARROS, N. F. de
700 1 $aBRIENZA JUNIOR, S.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.26, n.1, p.69-76, jan.1991
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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