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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PAMPLONA, R. de C. de A.; VILELA, E. S. D.; PANSA, C. C.; CASTELIANI, A. G. B.; MOITINHO, M. A.; MELO, I. S. de. |
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Afiliação: |
R. de C. de A. PAMPLONA, Hermínio Ometto University; E. S. D. VILELA, ESALQ/USP; C. C. PANSA, ESALQ/USP; A. G. B. CASTELIANI, ESALQ/USP; M. A. MOITINHO, ESALQ/USP. |
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Título: |
Antifungal activity of the isolated from caatinga actinobacteria in control of Pythium aphanidermatum. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0969-2. |
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Idioma: |
Inglês |
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Conteúdo: |
The Caatinga is a biome located in a semiarid region of northeastern Brazil. It consists of various ecosystems, presented a unique environmental system of this country. Because it is an environment with unique characteristics, recent studies have revealed the private wealth of this biome in terms of microbial biodiversity. Microorganisms found in this habitat may present specific mechanisms to ensure their survival in this environment that provides extreme conditions, such as temperature changes, intense solar radiation and hydrous stress. Because of these different mechanisms used by different microorganisms, becomes an enabling environment to search for new enzymes and bioactive substances produced by these organisms can present different biotechnological applications. Among all microbial diversity, the group of actinobacteria is of great importance, because they are responsible for producing most of the known antibiotics and present as great producers of enzymes with various industrial applications. One possible application of compounds produced by this organism is the phytogenic fungi combat, such as rot caused in roots of plants by the fungus Pythium aphanidermatum. This study aimed to search actinobacteria from Caatinga biome with metabolic potential to work in the biological control of Pythium aphanidermatum fungus. With Caatinga soil samples, they were isolated actinomycetes differences in morphologies and subjected to extraction metabolites produced from solid culture medium. The extracts were tested on the P. aphanidermatum fungus diffused by drive test. From the isolation were recovered a total of 272 strains of actinomycetes, of this total 47% of the isolates showed in vitro activity against the pathogen Pythium aphanidermatum. The results showing the great potential that actinobacteria may present as control agents against root rot. However, more detailed studies are needed to prove such results. MenosThe Caatinga is a biome located in a semiarid region of northeastern Brazil. It consists of various ecosystems, presented a unique environmental system of this country. Because it is an environment with unique characteristics, recent studies have revealed the private wealth of this biome in terms of microbial biodiversity. Microorganisms found in this habitat may present specific mechanisms to ensure their survival in this environment that provides extreme conditions, such as temperature changes, intense solar radiation and hydrous stress. Because of these different mechanisms used by different microorganisms, becomes an enabling environment to search for new enzymes and bioactive substances produced by these organisms can present different biotechnological applications. Among all microbial diversity, the group of actinobacteria is of great importance, because they are responsible for producing most of the known antibiotics and present as great producers of enzymes with various industrial applications. One possible application of compounds produced by this organism is the phytogenic fungi combat, such as rot caused in roots of plants by the fungus Pythium aphanidermatum. This study aimed to search actinobacteria from Caatinga biome with metabolic potential to work in the biological control of Pythium aphanidermatum fungus. With Caatinga soil samples, they were isolated actinomycetes differences in morphologies and subjected to extraction metabolites produced from solid cultu... Mostrar Tudo |
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Thesagro: |
Caatinga; Pythium Aphanidermatum. |
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Thesaurus Nal: |
Actinobacteria. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139540/1/2015RA-017.pdf
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Marc: |
LEADER 02690nam a2200205 a 4500
001 2037848
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aPAMPLONA, R. de C. de A.
245 $aAntifungal activity of the isolated from caatinga actinobacteria in control of Pythium aphanidermatum.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0969-2.$c0969
520 $aThe Caatinga is a biome located in a semiarid region of northeastern Brazil. It consists of various ecosystems, presented a unique environmental system of this country. Because it is an environment with unique characteristics, recent studies have revealed the private wealth of this biome in terms of microbial biodiversity. Microorganisms found in this habitat may present specific mechanisms to ensure their survival in this environment that provides extreme conditions, such as temperature changes, intense solar radiation and hydrous stress. Because of these different mechanisms used by different microorganisms, becomes an enabling environment to search for new enzymes and bioactive substances produced by these organisms can present different biotechnological applications. Among all microbial diversity, the group of actinobacteria is of great importance, because they are responsible for producing most of the known antibiotics and present as great producers of enzymes with various industrial applications. One possible application of compounds produced by this organism is the phytogenic fungi combat, such as rot caused in roots of plants by the fungus Pythium aphanidermatum. This study aimed to search actinobacteria from Caatinga biome with metabolic potential to work in the biological control of Pythium aphanidermatum fungus. With Caatinga soil samples, they were isolated actinomycetes differences in morphologies and subjected to extraction metabolites produced from solid culture medium. The extracts were tested on the P. aphanidermatum fungus diffused by drive test. From the isolation were recovered a total of 272 strains of actinomycetes, of this total 47% of the isolates showed in vitro activity against the pathogen Pythium aphanidermatum. The results showing the great potential that actinobacteria may present as control agents against root rot. However, more detailed studies are needed to prove such results.
650 $aActinobacteria
650 $aCaatinga
650 $aPythium Aphanidermatum
700 1 $aVILELA, E. S. D.
700 1 $aPANSA, C. C.
700 1 $aCASTELIANI, A. G. B.
700 1 $aMOITINHO, M. A.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARTINS, K. R.; GARCIA, M. V.; BONATTE JUNIOR, P.; DUARTE, P. O.; HIGA, L. O. S. de; CSORDAS, B. G.; BARROS, J. C.; ANDREOTTI, R. |
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Afiliação: |
Kauê Rodriguez Martins; Marcos Valério Garcia; Paulino Bonatte?Junior; Pãmella Oliveira Duarte; Leandro Oliveira Souza de Higa; Bárbara Guimarães Csordas; JACQUELINE CAVALCANTE BARROS, CNPGC; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, jun. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation. MenosThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable ... Mostrar Tudo |
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Palavras-Chave: |
IELISA; QPCR; Tick. |
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Thesagro: |
Cerrado. |
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Thesaurus NAL: |
Anaplasmosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219597/1/16-Correlation-between.pdf
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Marc: |
LEADER 02269naa a2200265 a 4500
001 2128625
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aMARTINS, K. R.
245 $aCorrelation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil.$h[electronic resource]
260 $c2020
520 $aThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation.
650 $aAnaplasmosis
650 $aCerrado
653 $aIELISA
653 $aQPCR
653 $aTick
700 1 $aGARCIA, M. V.
700 1 $aBONATTE JUNIOR, P.
700 1 $aDUARTE, P. O.
700 1 $aHIGA, L. O. S. de
700 1 $aCSORDAS, B. G.
700 1 $aBARROS, J. C.
700 1 $aANDREOTTI, R.
773 $tExperimental and Applied Acarology, jun. 2020.
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