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| Registros recuperados : 161 | |
| 64. |  | ANDREOTTI, R.; BARROS, J. C.; SILVA, E. A. e; AZZOLINI, S. S.; TANAKA, A. Inibidores de serinoproteases presentes na fase de vida livre do carrapato Rhipicephalus sanguineus. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 323, 2004. Suplemento 1, ref. 453. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 65. | | GONÇALVES, K. N.; LOCATELLI-DITTRICH, R.; MELLO, H. J. H. de; BARROS, J. C.; ANDREOTTI, R. Infecção por N. caninum em rebanho ovino do Mato Grosso do Sul. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 223, 2004. Suplemento 1, ref. 067. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 67. | | SILVA, E. A.; ANDREOTTI, R.; DIAS, E. S.; BARROS, J. C.; BRAZUNA, J. C. M. Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil. Experimental Parasitology, New York, v. 119, p. 343-348, July 2008. Issue 3.| Biblioteca(s): Embrapa Gado de Corte. |
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| 73. | | BARROS, J. C.; FIGUEIREDO NETO, L. F.; CUNHAI, R. C.; ANDREOTTI, R. Diagnóstico da perda econômica causada pela neosporose na reprodução de novilhas de corte. In: CONGRESSO SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 48., 2010, Campo Grande, MS. Tecnologias, desenvolvimento e integração social. Campo Grande, MS: Sociedade Brasileira de Economia, Administração e Sociologia Rural, 2010. 13 p. SOBER| Biblioteca(s): Embrapa Gado de Corte. |
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| 74. | | SOARES, M. A.; ANDREOTTI, R.; BARROS, J. C.; MELLO, A. K. M. Eficiência in vitro de acaricidas em cepas de campo de carrapato-do-boi. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 76. | | HIGA, L. de O. S.; GARCIA, M. V.; BARROS, J. C.; KOLLER, W. W.; ANDREOTTI, R. Evaluation of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) resistance to different acaricide formulations using samples from brazilian properties. Revista Brasileira de Parasitologia Veterinária , Jaboticabal, v. 25, n. 2, p. 163-171, abr./jul. 2016.| Biblioteca(s): Embrapa Gado de Corte. |
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| 79. | | ANDREOTTI, R.; PINHEIRO, M. S.; SANTOS, S. A.; AZEVEDO, J. R. M.; BARROS, J. C. Observacoes sobre a preferencia termica de agua pelo jacare do Pantanal (Caiman crocodilus yacare, Daudin, 1802). In: CONGRESSO PANAMERICANO DE CIENCIAS VETERINARIAS, 15., 1996, Campo Grande. Abstracts... Campo Grande: SOMVET-MS / CRMV-MS, 1996. p.82| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 161 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARTINS, K. R.; GARCIA, M. V.; BONATTE JUNIOR, P.; DUARTE, P. O.; HIGA, L. O. S. de; CSORDAS, B. G.; BARROS, J. C.; ANDREOTTI, R. |
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Afiliação: |
Kauê Rodriguez Martins; Marcos Valério Garcia; Paulino Bonatte?Junior; Pãmella Oliveira Duarte; Leandro Oliveira Souza de Higa; Bárbara Guimarães Csordas; JACQUELINE CAVALCANTE BARROS, CNPGC; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, jun. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation. MenosThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable ... Mostrar Tudo |
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Palavras-Chave: |
IELISA; QPCR; Tick. |
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Thesagro: |
Cerrado. |
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Thesaurus NAL: |
Anaplasmosis. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1128625/1/16-Correlation-between.pdf
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Marc: |
LEADER 02269naa a2200265 a 4500
001 2128625
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aMARTINS, K. R.
245 $aCorrelation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil.$h[electronic resource]
260 $c2020
520 $aThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation.
650 $aAnaplasmosis
650 $aCerrado
653 $aIELISA
653 $aQPCR
653 $aTick
700 1 $aGARCIA, M. V.
700 1 $aBONATTE JUNIOR, P.
700 1 $aDUARTE, P. O.
700 1 $aHIGA, L. O. S. de
700 1 $aCSORDAS, B. G.
700 1 $aBARROS, J. C.
700 1 $aANDREOTTI, R.
773 $tExperimental and Applied Acarology, jun. 2020.
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