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| 87. |  | FERNANDES, E. N.; SILVA, C. A. B. da; COUTO, L. Sistema especialista para planejamento e desenho de sistemas agroflorestais. In: CONGRESSO BRASILEIRO SOBRE SISTEMAS AGROFLORESTAIS, 1.; ENCONTRO SOBRE SISTEMAS AGROFLORESTAIS NOS PAÍSES DO MERCOSUL, 1., 1994, Porto Velho. Anais. Colombo: EMBRAPA-CNPF, 1994. v. 2, p. 337-344. (EMBRAPA-CNPF. Documentos, 27).| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 393 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
12/12/2011 |
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Data da última atualização: |
24/01/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, T. L. P. O.; BARROS, E. G. de; BELLATO, C. M.; HWANG, E.-Y.; CREGAN, P. B.; PASTOR-CORRALES, M. A. |
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Afiliação: |
THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; EVERALDO G. DE BARROS, UNIVERSIDADE FEDERAL DE VIÇOSA; CLAUDIA M. BELLATO, USDA; EUN-YOUNG HWANG, USDA; PERRY B. CREGAN, USDA; MARCIAL A. PASTOR-CORRALES, USDA. |
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Título: |
Single nucleotide polymorphism discovery in common bean. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Breeding, Dordrecht, v. 30, p. 419-428, 2012. |
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DOI: |
DOI 10.1007/s11032-011-9632-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species. MenosSingle nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies a... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Phaseolus vulgaris; Polimorfismo genético. |
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Thesaurus NAL: |
Genome; Polymerase chain reaction; Single nucleotide polymorphism. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02303naa a2200277 a 4500
001 1909254
005 2013-01-24
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11032-011-9632-4$2DOI
100 1 $aSOUZA, T. L. P. O.
245 $aSingle nucleotide polymorphism discovery in common bean.
260 $c2012
520 $aSingle nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species.
650 $aGenome
650 $aPolymerase chain reaction
650 $aSingle nucleotide polymorphism
650 $aDNA
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aPolimorfismo genético
700 1 $aBARROS, E. G. de
700 1 $aBELLATO, C. M.
700 1 $aHWANG, E.-Y.
700 1 $aCREGAN, P. B.
700 1 $aPASTOR-CORRALES, M. A.
773 $tMolecular Breeding, Dordrecht$gv. 30, p. 419-428, 2012.
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