Data/hora: 19/06/2024 00:58:31

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 02/07/2013
Data da última atualização: | 13/06/2017
Autoria: | RIBEIRO, C. A. G.; TANURE, J. P. M.; MACIEL, T. E. F.; BARROS, E. G. de.
Afiliação: | CALOS ALEXANDRE GOMES RIBEIRO, UFV; JANAÍNA PAULA MARQUES TANURE, Embrapa Gado de Corte; TALLES EDUARDO FERREIRA MACIEL, UFV; EVERALDO GONÇALVES DE BARROS, UFV.
Título: | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence.
Ano de publicação: | 2013
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 3, p. 270-279, mar. 2013.
Idioma: | Inglês
Notas: | Título em português: Caracterização molecular de cultivares de soja por meio de marcadores  microssatélites com sequência de cauda universal.
Conteúdo: | The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2?7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2?5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the etermination of genetic purity.
Palavras-Chave: | Cultivar protection; Diversidade genética; Genotyping method; Método de genotipagem; Probabilidade de identidade ao acaso; Proteção de cultivar; Random identity probability.
Thesagro: | Glycine Max.
Thesaurus Nal: | Genetic variation.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/85161/1/Molecular-characterization.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (54814-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS