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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/07/1995 |
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Data da última atualização: |
05/07/1995 |
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Autoria: |
SAKAI, A.; KOBAYASHI, S.; OIYAMA, I. |
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Afiliação: |
Asabucho 1-5-23, Sapporo, 001 Japan. Akitsu Branch, Fruit Tree Research Station, Akitsu, Hiroshima, 729-24 Japan. |
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Título: |
Survival by vitrification of nucellar cells of navel orange (citrus sinensis var. brasiliensis tanaka)cooled to - 196 degree C |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
J. Plant Physiol, v.137, p.465-570, 1991 |
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Idioma: |
Inglês |
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Conteúdo: |
The nucellar cells of navel orange (Citrus sinensis Obs. var. brasiliensis Tanaka) with potential for somatic embryo production have been successfully cryopreserved for 40 days by vitrification. In this method, cells were sufficiently dehydrated with a highly concentrated cryoprotective solution (designated PVS2) to be capable of vitrifying during rapid cooling in liqued nitrogen. The cells treated with PVS2 at 25 degree C for 3 min were transferred to a 1.8 mL plastic cryotube and then directly plunged into liquid nitrogen. The average rate of survival was about 90%. The revived cells resumed growth within 3 days and produced cotyledonary embryoids that developed into plants 3 months of culture. This simple method has been successfully applied to six other species or cultivars with high rates of survival. The time used for the procedure is only several minutes. This novel method for cryopreservation seems promising as a practical method applicable a wide range of cultured plant cells and meristems. |
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Palavras-Chave: |
Brasiliensis cryopreservation; Citrus sinensis Obs; Navel orange; Nucellar cells; Var. |
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Thesaurus Nal: |
vitrification. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01649naa a2200217 a 4500
001 1646627
005 1995-07-05
008 1991 bl --- 0-- u #d
100 1 $aSAKAI, A.
245 $aSurvival by vitrification of nucellar cells of navel orange (citrus sinensis var. brasiliensis tanaka)cooled to - 196 degree C
260 $c1991
520 $aThe nucellar cells of navel orange (Citrus sinensis Obs. var. brasiliensis Tanaka) with potential for somatic embryo production have been successfully cryopreserved for 40 days by vitrification. In this method, cells were sufficiently dehydrated with a highly concentrated cryoprotective solution (designated PVS2) to be capable of vitrifying during rapid cooling in liqued nitrogen. The cells treated with PVS2 at 25 degree C for 3 min were transferred to a 1.8 mL plastic cryotube and then directly plunged into liquid nitrogen. The average rate of survival was about 90%. The revived cells resumed growth within 3 days and produced cotyledonary embryoids that developed into plants 3 months of culture. This simple method has been successfully applied to six other species or cultivars with high rates of survival. The time used for the procedure is only several minutes. This novel method for cryopreservation seems promising as a practical method applicable a wide range of cultured plant cells and meristems.
650 $avitrification
653 $aBrasiliensis cryopreservation
653 $aCitrus sinensis Obs
653 $aNavel orange
653 $aNucellar cells
653 $aVar
700 1 $aKOBAYASHI, S.
700 1 $aOIYAMA, I.
773 $tJ. Plant Physiol$gv.137, p.465-570, 1991
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
