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| Registros recuperados : 192 | |
| 61. |  | BARRETO, M. R.; LOGUERCIO, L. L.; TEIXEIRA, F. F.; VALICENTE, F. H.; PAIVA, E. Susceptibilidade da lagarta do cartucho do milho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) a isolados de baculovirus. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 20., 1999, Salvador. Resumos... Salvador: SBM, 1999.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 62. | | BARRETO, M. A.; ALMEIDA, M. A. O. de; SILVA, A.; BITENCUR, C. P. Avaliação da resistência anti-helmíntica de nematódeos gastrintestinais em ovinos deslanados do estado da Bahia. Revista Brasileira de Parasitologia Veterinária, v. 13, p. 265, 2004. Suplemento 1. Resumo 229. Edição de anais do 13o. Congresso Brasileiro de Parasitologia Veterinária e o 1o. Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 65. | | PEZZINI, L. A.; BARRETO, M. R.; QUEIROZ, D. L. de; BURCKHARDT, D. Diversidade de psilídeos na região de Sinop. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., 2016, Maceió. Anais... Brasília, DF : Embrapa, 2016. p. 168.| Biblioteca(s): Embrapa Florestas. |
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| 66. | | LOPES, M. V.; OLIVEIRA, C. A. L.; BARRETO, M.; BARBOSA, J. C.; ROSSI, F. M. Dimensionamento de amostras para monitoramento do ácaro da leprose Brevipalpus phoenicis (Geijskes, 1939) em citros. Revista Brasileira de Fruticultura, Jaboticabal, v. 29, n. 3, p. 671-676, dez. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 68. | | BARRETO, M. R.; TEIXEIRA, F. F.; LOGUERCIO, L. L.; VALICENTE, F. H.; PAIVA, E. Efeito de isolados de Baculovirus em lagartas de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) e sua caracterização utilizando PCR. Genetics and Molecular Biology, Ribeirão Preto, v. 22, n. 3, p. 430, 1999. Suplemento. Edição dos resumos do Congresso Nacional de Genética, Gramado, 1999.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 70. | | BARRETO, M. R.; GUIMARAES, C. T.; TEIXEIRA, F. F.; PAIVA, E.; VALICENTE, F. H. Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. Neotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 71. | | MOREIRA, M. A.; MAGALHAES, B. P.; VICENTINI, S.; BARRETO, M. F. P.; FRAZAO, H. S. Efeito do fungo Metarhizium flavoviride sobre Stiphra robusta (Orthoptera: Proscopiidae) em condicoes de campo. In: SIMPOSIO DE CONTROLE BIOLOGICO, 5., 1996, Foz do Iguacu, PR. Anais: sessao de posters. Londrina: EMBRAPA-CNPSO, 1996. p.265.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 73. | | MAZZARDO, T.; BARRETO, M. R.; QUEIROZ, D. L. de; BURCKHARDT, D. Diversity and distribution of jumping plant-lice (Hemiptera: Psylloidea) along edges of Amazon - Cerrado transitional forests in Sorriso, Mato Grosso, Brazil. Check List, v. 13, n. 3, article 2131, 2017. 7 p.| Biblioteca(s): Embrapa Florestas. |
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| 77. | | MOREIRA, M.; CHAGAS, M.; MAGALHAES, B.; VICENTINE, S.; BARRETO, M. de F. Acao de Metarhizium flavoviride sobre Schistocerca pallens em condicoes de campo no Rio Grande do Norte. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 15., 1995, Caxambu, MG. Resumos. [S.l.]: Sociedade Entomologica do Brasil, 1995. p.410. p.410 Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 78. | | LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E. Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 192 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/03/2022 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PAULA, D. P.; BARROS, S. K. A.; PITTA, R. M.; BARRETO, M. R.; TOGAWA, R. C.; ANDOW, D. A. |
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Afiliação: |
DEBORA PIRES PAULA, Cenargen; SUELLEN KARINA ALBERTONI BARROS, UFMT; RAFAEL MAJOR PITTA, CPAMT; MARLITON ROCHA BARRETO, UFMT; ROBERTO COITI TOGAWA, Cenargen; DAVID A. ANDOW, UNIVERSITY OF MINNESOTA. |
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Título: |
Metabarcoding versus mapping unassembled shotgun reads for identification of prey consumed by arthropod epigeal predators. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
GigaScience, v. 11, n. 1, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1093/gigascience/giac020 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while metabarcoding did not. MenosAbstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while ... Mostrar Tudo |
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Palavras-Chave: |
Diet analysis; Environmental DNA; Generalist predators; Gut content analysis. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232999/1/giac020.pdf
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Marc: |
LEADER 02285naa a2200241 a 4500
001 2151304
005 2023-01-27
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1093/gigascience/giac020$2DOI
100 1 $aPAULA, D. P.
245 $aMetabarcoding versus mapping unassembled shotgun reads for identification of prey consumed by arthropod epigeal predators.$h[electronic resource]
260 $c2022
520 $aAbstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while metabarcoding did not.
653 $aDiet analysis
653 $aEnvironmental DNA
653 $aGeneralist predators
653 $aGut content analysis
700 1 $aBARROS, S. K. A.
700 1 $aPITTA, R. M.
700 1 $aBARRETO, M. R.
700 1 $aTOGAWA, R. C.
700 1 $aANDOW, D. A.
773 $tGigaScience$gv. 11, n. 1, p. 1-13, 2022.
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Embrapa Agrossilvipastoril (CPAMT) |
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