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3. | | LOPES, V. S.; FUGANTI, R.; BARBOSA, J. F.; DE CARVALHO, M. C. C. G.; MARIN, S. R. R.; SILVA, J. F. V.; NEPOMUCENO, A. L.; MARCELINO, F. C. Estudos in vivo do papel de inserções AT(n) da região promotora do gene Gmhsp17.6-L na regulação de genes em soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 169-174. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo. Biblioteca(s): Embrapa Soja. |
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4. | | SILVA, E. M. S. da; RIBEIRO, D. D.; NASCIMENTO, L. dos S.; LIMA, Y. S.; CORREIA, R. C.; BARBOSA, J. F.; SILVA, T. M. S. da. Levantamento quantitativo e fatores limitantes da produção de mel do município de Casa Nova - BA. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 27., 2017, Santos. Anais... Brasília, DF: Associação Brasileira de Zootecnistas, 2017. Biblioteca(s): Embrapa Semiárido. |
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5. | | SILVA, E. M. S. da; NASCIMENTO, L. dos S.; RIBEIRO, D. D.; LIMA, Y. S.; BARBOSA, J. F.; CORREIA, R. C.; SILVA, T. M. S. da. Levantamento de técnicas de manejo realizado pelo os apicultores do município de Sento Sé - BA. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 27., 2017, Santos. Anais... Brasília, DF: Associação Brasileira de Zootecnistas, 2017. Biblioteca(s): Embrapa Semiárido. |
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6. | | GEORG, E. S.; CARVALHO, F. I. F. de; BARBOSA, J. F.; FRANCO, F. A.; FERREIRA FILHO, A. W. P.; REIS, M. S.; VIAU, L. V. M. Desempenho preliminar de novos genotipos de aveia e trigo na depressao central do RS. Pesquisa Agropecuaria Brasileira, Brasilia, v.21, n.11, p.1169-1175, nov. 1986. Biblioteca(s): Embrapa Trigo; Embrapa Unidades Centrais. |
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7. | | CARVALHO, F. I. F.; BARBOSA, J. F.; FLOSS, E. L.; PERIERA FILHO, A. W. P.; FRANCO, F. de A.; FEDERIZZI, L. C.; NODARI, R. O. Potencial Genético da Aveia, como Produtora de Grãos, no Sul do Brasil. Pesquisa Agropecuária Brasileira, Brasília, v.22, n.1, p.71-82, jan.1987 Biblioteca(s): Embrapa Unidades Centrais. |
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8. | | CARVALHO, F. I. F.; BARBOSA, J. F.; FOLSS, E. L.; FERREIRA FILHO, A. W. P.; FRANCO, F. de A.; FEDERIZZI, L. C.; NODARI, R. O. Potencial Genético da Aveia, como Produtora de Grãos, no Sul do Brasil. Pesquisa Agropecuária Brasileira, Brasília, v.22, n.1, p. 71-82, jan. 1987. Biblioteca(s): Embrapa Unidades Centrais. |
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9. | | MORALES, A. M. A. P.; PEREIRA, A. A.; MURAKAMI, A. Y.; ROCHA, L. K. V.; STOLF, R.; BARBOSA, J. F.; ABDELNOOR, R. V.; MARCELINO, F. C.; BOREM, A.; LOUREIRO, M. E. Construção de biblioteca full length de soja inoculada com o fungo Phakopsora pachyrhizi. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia: [anais...]. Fortaleza: UFC: Embrapa Agroindústria Tropical, 2009. Seção trabalhos, 15.pdf. 1 CD-ROM. CBFV 2009. Biblioteca(s): Embrapa Soja. |
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10. | | KIDO, É. A.; MARCELINO, F. C.; NASCIMENTO, L. C. do; SILVA, A. B. da; CARAZZOLLE, M. F.; BINNECK, E.; STOLF, R.; BARBOSA, J. F.; NEPOMUCENO, A. L.; JÚNIOR, T. C.; PEREIRA, G. A. G.; ABDELNOOR, R. V. Transcript analysis of soybean plants in response to Asian rust infection. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 145, ref. P376. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng. Biblioteca(s): Embrapa Soja. |
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11. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. BMC Genomics, v. 12, n. 307, jun. 2011. 17 p. Biblioteca(s): Embrapa Soja. |
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12. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERÃO, M.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 12 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/01/2012 |
Data da última atualização: |
03/04/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. |
Afiliação: |
FRANCELI R. KULCHESKI, UFRGS; LUIZ F. V. DE OLIVEIRA, UFRGS; LORRAYNE, G. MOLINA, UFRGS; MAURÍCIO P. ALMERÃO, UFRGS; FABIANA A. RODRIGUES, CNPSo; JULIANA MARCOLINO, CNPSo; JOICE F. BARBOSA, CNPSo; RENATA STOLF MOREIRA, CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; LEANDRO C. NASCIMENTO, UNICAMP; MARCELO F. CARAZZOLLE, UNICAMP; GONÇALO A. G. PEREIRA, UNICAMP; ROGÉRIO MARGIS, UFRGS. |
Título: |
Identification of novel soybean microRNAs involved in abiotic and biotic stresses. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
BMC Genomics, v. 12, n. 307, jun. 2011. |
Páginas: |
17 p. |
DOI: |
10.1186/1471-2164-12-307 |
Idioma: |
Português |
Conteúdo: |
Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. MenosBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also di... Mostrar Tudo |
Thesagro: |
Deficiência hídrica; Gene marcador; Soja. |
Thesaurus NAL: |
Gene expression; Soil water deficit; Soybeans. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51756/1/BMC-2011.pdf
|
Marc: |
LEADER 03216naa a2200385 a 4500 001 1911802 005 2013-04-03 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1186/1471-2164-12-307$2DOI 100 1 $aKULCHESKI, F. R. 245 $aIdentification of novel soybean microRNAs involved in abiotic and biotic stresses. 260 $c2011 300 $a17 p. 520 $aBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. 650 $aGene expression 650 $aSoil water deficit 650 $aSoybeans 650 $aDeficiência hídrica 650 $aGene marcador 650 $aSoja 700 1 $aOLIVEIRA, L. F. V. 700 1 $aMOLINA, L. G. 700 1 $aALMERAO, M. P. 700 1 $aRODRIGUES, F. A. 700 1 $aMARCOLINO, J. 700 1 $aBARBOSA, J. F. 700 1 $aSTOLF-MOREIRA, R. 700 1 $aNEPOMUCENO, A. L. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aABDELNOOR, R. V. 700 1 $aNASCIMENTO, L. C. 700 1 $aCARAZZOLLE, M. F. 700 1 $aPEREIRA, G. A. G. 700 1 $aMARGIS, R. 773 $tBMC Genomics$gv. 12, n. 307, jun. 2011.
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